Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of chicken insulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
92
Range %
87-98
1:2
Average %
96
Range %
90-101
1:4
Average %
98
Range %
95-103
1:8
Average %
90
Range %
85-96
回收率:
The recovery of chicken insulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-100
EDTA plasma (n=4)
97
95-101
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Regarding your ELISA kit CSB-E13293C, could you please advise on the following: 1) Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression? 2) Does the standard include additive agent such as antiseptic or preservative? If it includes, what is the composition of said additive agent? 3) Material name and concentration of Stop Solution. 4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?
A:
Thanks for your inquiry. CSB-E13293C Standard:293 cell-derived recombinant protein. Stop solution is 4N sulfuric acid. Preservative is proclin 300(1:3000). The kit doesn't contain hazardous substance such as azide, thimerosal, 2-ME, etc. Pls let me know if you have any further questions. Thank you.
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
基因功能參考文獻:
Data suggest that insulin signaling in skeletal muscle can be regulated by dietary factors (here, dietary content of omega-3 fatty acids). PMID: 24094087
Polymorphisms within the INS gene are associated with 55-week body weight. PMID: 16734694
The present findings suggest the involvement of insulin in neuronal/glial coupling in the cerebellum. PMID: 19135128