Chicken Leptin,LEP ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞瘦素(LEP)酶聯(lián)免疫試劑盒（CSB-E14082C）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LEP含量。LEP（瘦素）是一種由脂肪細(xì)胞分泌的蛋白質(zhì)類激素，通過抑制饑餓來調(diào)控能量平衡。其研究機(jī)制涉及與瘦素受體結(jié)合，激活下游信號(hào)通路，抑制進(jìn)食并促進(jìn)能量消耗。LEP的研究對(duì)肥胖、糖尿病等代謝性疾病有重要意義。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，科研人員可通過本試劑盒開展家禽生長(zhǎng)調(diào)控機(jī)制、脂肪代謝通路、繁殖生理學(xué)以及營(yíng)養(yǎng)干預(yù)等基礎(chǔ)研究，為禽類健康管理及遺傳育種提供實(shí)驗(yàn)依據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
縮寫：

LEP
Uniprot No.：

O42164
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-101
1:2	Average %	95
	Range %	85-104
1:4	Average %	91
	Range %	87-100
1:8	Average %	93
	Range %	89-106

回收率：

The recovery of chicken LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-107
EDTA plasma (n=4)	95	88-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Leptin family
組織特異性：

Not exclusively localized in adipose tissue but is also expressed in liver.