Dog Interleukin-33(IL33) ELISA kit
-
中文名稱：犬白介素33(IL33)酶聯(lián)免疫試劑盒
-
貨號(hào)：CSB-EL011656DO
-
規(guī)格：96T/48T
-
價(jià)格：￥4200/￥3000
-
其他：
產(chǎn)品詳情
-
產(chǎn)品描述：犬白介素33(IL33)酶聯(lián)免疫試劑盒（CSB-EL011656DO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL33含量。IL33是重要靶點(diǎn)，其作為白細(xì)胞介素家族成員，在免疫調(diào)節(jié)中起關(guān)鍵作用。研究發(fā)現(xiàn)它能激活下游信號(hào)通路，調(diào)控免疫細(xì)胞功能。異常表達(dá)與哮喘、炎癥性腸病等多種炎癥疾病相關(guān)，對(duì)其深入研究有助于開(kāi)發(fā)新的免疫治療策略。試劑盒檢測(cè)范圍為23.44 pg/mL-1500 pg/mL，本試劑盒可用于探索犬類疾病模型中的免疫調(diào)控機(jī)制、炎癥相關(guān)研究或藥物干預(yù)實(shí)驗(yàn)中的生物標(biāo)志物檢測(cè)。其操作流程兼容常規(guī)實(shí)驗(yàn)室條件，可為獸醫(yī)基礎(chǔ)研究、比較醫(yī)學(xué)或?qū)嶒?yàn)動(dòng)物模型開(kāi)發(fā)提供可靠的數(shù)據(jù)支持，幫助科研人員深入解析IL33在犬類免疫系統(tǒng)中的功能及調(diào)控網(wǎng)絡(luò)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
-
別名：IL33Interleukin-33 ELISA Kit; IL-33 ELISA Kit; Protein DVS27 ELISA Kit
-
縮寫(xiě)：
-
Uniprot No.：
-
種屬：Canis lupus familiaris (Dog) (Canis familiaris)
-
樣本類型：serum, plasma, tissue homogenates
-
檢測(cè)范圍：23.44 pg/mL-1500 pg/mL
-
靈敏度：5.86 pg/mL
-
反應(yīng)時(shí)間：1-5h
-
樣本體積：50-100ul
-
檢測(cè)波長(zhǎng)：450 nm
-
研究領(lǐng)域：Immunology
-
測(cè)定原理：quantitative
-
測(cè)定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 82-89 1:2 Average % 102 Range % 97-107 1:4 Average % 89 Range % 85-93 1:8 Average % 96 Range % 93-90 -
回收率：
The recovery of dog IL33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 88-95 EDTA plasma (n=4) 97 90-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1500 2.929 2.968 2.949 2.853 750 2.406 2.300 2.353 2.257 375 1.631 1.645 1.638 1.542 187.5 0.915 0.935 0.925 0.829 93.75 0.506 0.500 0.503 0.407 46.88 0.254 0.275 0.265 0.169 23.44 0.141 0.152 0.147 0.051 0 0.094 0.098 0.096 -
數(shù)據(jù)處理：
-
貨期：3-5 working days
引用文獻(xiàn)
- Unraveling the complex relationship between mRNA and protein abundances: a machine learning-based approach for imputing protein levels from RNA-seq data EG Metaab,NAR genomics and bioinformatics,2024
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
功能：Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
-
亞細(xì)胞定位：Nucleus. Chromosome. Cytoplasm. Cytoplasmic vesicle, secretory vesicle. Secreted.
-
蛋白家族：IL-1 family
-
組織特異性：Expressed in cultured umbilical artery smooth muscle cells after stimulation with IL1A and IL1B, and to a lesser extent with IFNG. Expressed in vasospastic cerebral arteries after subarachnoid hemorrhage.
-
數(shù)據(jù)庫(kù)鏈接：
KEGG: cfa:403810
UniGene: Cfa.3672
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
