Dog growth hormone,GH ELISA Kit
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中文名稱：犬生長激素(GH)酶聯(lián)免疫試劑盒
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貨號：CSB-E14962c
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規(guī)格：96T/48T
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價(jià)格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：犬生長激素(GH)酶聯(lián)免疫試劑盒（CSB-E14962c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 即生長激素 1 基因，它編碼生長激素，在人體生長發(fā)育、代謝等生理過程中扮演重要角色。研究機(jī)制方面，主要圍繞其調(diào)控生長激素合成與分泌、與生長信號通路的相互作用等，這些研究有助于深入理解生長發(fā)育異常機(jī)制及相關(guān)疾病治療。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品適用于動(dòng)物生長機(jī)制研究、代謝調(diào)控分析、藥物或營養(yǎng)干預(yù)實(shí)驗(yàn)等科研領(lǐng)域，為探究犬類生理病理過程中的GH動(dòng)態(tài)變化提供穩(wěn)定工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：GH1 ELISA Kit; GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：0.625 ng/mL-40 ng/mL
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靈敏度：0.156 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of dog GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
92
Range %
88-97
1:2
Average %
98
Range %
91-105
1:4
Average %
89
Range %
84-95
1:8
Average %
99
Range %
92-106
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回收率：
The recovery of dog GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
92
85-99
EDTA plasma (n=4)
99
92-106
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標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
40
2.853
2.734
2.794
2.632
20
2.087
2.067
2.077
1.916
10
1.504
1.498
1.501
1.339
5
0.897
0.929
0.913
0.751
2.5
0.550
0.521
0.536
0.374
1.25
0.383
0.363
0.373
0.211
0.625
0.271
0.284
0.277
0.116
0
0.158
0.165
0.162
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數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
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基因功能參考文獻(xiàn)：
- Progesterone alone has no direct stimulatory effect on GH transcription in mammary gland. PMID: 18262383
- Study provides evidence that progesteron might increase autocrine Growth Hormone production which might directly stimulate local or systemic IGF-I secretion in canine mammary tumours. PMID: 18367392
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亞細(xì)胞定位：Secreted.
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蛋白家族：Somatotropin/prolactin family
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數(shù)據(jù)庫鏈接：
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