Mouse osteonectin,ON ELISA Kit
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中文名稱：小鼠骨粘連蛋白(ON)酶聯(lián)免疫試劑盒
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貨號：CSB-E08762m
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規(guī)格：96T/48T
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價格：￥3800/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠骨粘連蛋白(ON)酶聯(lián)免疫試劑盒（CSB-E08762m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SPARC含量。SPARC 即富含半胱氨酸的酸性分泌蛋白，在多種生理和病理過程中發(fā)揮作用。其背景在于它參與細(xì)胞外基質(zhì)的構(gòu)建和重塑。研究機制上，SPARC 可調(diào)節(jié)細(xì)胞與基質(zhì)間相互作用、影響細(xì)胞增殖與遷移，還和腫瘤的生長、轉(zhuǎn)移及預(yù)后相關(guān)，是潛在研究靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外培養(yǎng)細(xì)胞分泌功能分析、骨代謝相關(guān)疾病模型研究、組織再生機制探索等科研領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Sparc ELISA Kit; SPARC ELISA Kit; Basement-membrane protein 40 ELISA Kit; BM-40 ELISA Kit; Osteonectin ELISA Kit; ON ELISA Kit; Secreted protein acidic and rich in cysteine ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍：31.25 pg/mL-2000 pg/mL
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靈敏度：7.81 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Cancer
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 86 Range % 83-89 1:200 Average % 87 Range % 82-95 1:400 Average % 96 Range % 94-98 1:800 Average % 97 Range % 91-103 -
回收率：
The recovery of mouse ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 95-104 EDTA plasma (n=4) 91 87-95 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2000 2.477 2.479 2.478 2.342 1000 2.147 2.179 2.163 2.027 500 1.694 1.672 1.683 1.547 250 1.002 1.056 1.029 0.893 125 0.633 0.659 0.646 0.510 62.5 0.328 0.339 0.334 0.198 31.25 0.224 0.214 0.219 0.083 0 0.135 0.137 0.136 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
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基因功能參考文獻(xiàn)：
- It is a matricellular glycol that regulates cell proliferation, tissue repair, and tumorigenesis and a potential therapeutic target for conditions involving intestinal inflammation. PMID: 28582593
- Sparc levels in tendons are critical for proper collagen fibril maturation. PMID: 27586416
- miR-29 directly targeted SPARC, resulting in degradation of SPARC-encoding mRNA and reduction in the SPARC protein level. PMID: 29462611
- SPARC plays a crucial role in the proliferation and differentiation of C2C12 and may be involved in the link between the ECM remodeling and mitochondrial func PMID: 29420632
- SPARC is regulating the interplay between myeloid-derived suppressor cells and the extracellular matrix to drive the induction of epithelial-to-mesenchymal transition in tumor cells. PMID: 27681434
- The results demonstrate for the first time a functional role of the N-propeptide in regulating collagen fiber assembly and cell behavior and suggest that SPARC and the N-propeptide of collagen I have distinct activities in regulating collagen fiber assembly and fibroblast function. PMID: 28245286
- SPARC plays a key role in influencing the spatial organization of the anterior segment, potentially via modulation of collagen properties, while Hevin is not likely to be involved. PMID: 28122087
- An ADAMTS1 blocking antibody suppressed the SPARC-induced collagen I secretion, indicating that SPARC promoted collagen production directly through ADAMTS1 interaction. In conclusion, ADAMTS1 is an important mediator of SPARC-regulated cardiac aging. PMID: 27143554
- SPARC appears to be an important modulator of the actin cytoskeleton, implicating maintenance of muscular function PMID: 27908613
- resistivity measurements were taken on 22 mice, 11 wild-type and 11 sparc-/- (knock out for the protein SPARC: secreted protein acidic and rich in cysteine), bearing mammary carcinomas. PMID: 27099155
- SPARC isoforms, acting on Adipose stromal cells through distinct mechanisms, have an additive effect in inducing ASC migration. PMID: 26381424
- SPARC levels were not associated with efficacy in patients with MPC. This exploratory analysis does not support making treatment decisions regarding nab-paclitaxel plus gemcitabine or gemcitabine alone in MPC based on SPARC expression. PMID: 26169969
- SPARC is proposed to act as a critical regulator of transglutaminase activity on collagen I with implications for mechanical strength of tissues. PMID: 25827352
- Tumor-produced SPARC and VCAM1 are regulators of cancer extravasation. PMID: 25925867
- The results of this study indicated that the loss of p53 by deletion/mutation in the early stages of glioma formation may cooperate with the induction of SPARC to potentiate cancer cell survival and escape from immune surveillance. PMID: 24862407
- This work suggests that SPARC could affect anxiety-related behavior by modulating neuronal activity, and that depression-related behavior is dependent upon the adult expression of SPARC, which affects adult brain function. PMID: 22950524
- SPARC has a significant role in the development of concanavalin A -induced severe liver injury. PMID: 25410742
- SPARC acts as an important mediator of age-related cardiac inflammation by increasing the expression of macrophage M1 markers and decreasing M2 markers. PMID: 25877699
- SPARC inhibits growth factor-induced signaling in both INS-1 beta cells and primary mouse islets, and inhibits IGF-1-induced proliferation of INS-1 beta cells. PMID: 25204658
- Age and SPARC change the extracellular matrix composition of the left ventricle. PMID: 24783223
- findings suggest that SPARC plays a crucial role in the regulation of early B lymphopoiesis. PMID: 24598056
- SPARC-deficient mice display higher resistance to serial treatment with the chemotherapeutic agent 5-fluorouracil (5-FU). PMID: 24833352
- effects of age and the expression of SPARC on extracellular matrix production by cardiac fibroblasts PMID: 24223185
- SPARC KO mice had less inflammation. PMID: 24204877
- These results demonstrate that there is a delicate temporal regulation of SPARC and that disturbances in this may have an adverse effect on muscle regeneration. PMID: 23670848
- These findings suggest that SPARC plays a critical role in the maintenance of miMEFs without loss of cell number and might be a key component for supporting the culture of human PSCs. PMID: 23661086
- Loss of SPARC not only upregulates atrogin 1 expression but also enhances transforming growth factor (TGF)beta signaling, which may in turn cause muscle atrophy. PMID: 23424163
- data suggest that SPARC plays a significant role in liver fibrogenesis. Interventions to inhibit SPARC expression are suggested as promising approaches for liver fibrosis treatment PMID: 23408952
- new insight into the biological functions of SPARC PMID: 23349702
- These findings suggest that exercise stimulates SPARC secretion from muscle tissues and that SPARC inhibits colon tumorigenesis by increasing apoptosis. PMID: 22851666
- SPARC modulates intraocular pressure regulation in the eye, and also correlates with trabecular meshwork morphology. PMID: 23422826
- Using a chemical carcinogenesis model in Sparc-deficient mice and their wild-type littermates, we found that loss of SPARC accelerated the development of urothelial preneoplasia, neoplasia, and metastasis and was associated with decreased survival. PMID: 23321672
- This sdtudy demonistrated that SPARC regulates microgliosis and functional recovery following cortical ischemia and brain injury. PMID: 23467362
- SPARC and SC1 may share significant homology but are likely to have distinct but complementary roles in central nervous system development. PMID: 22173850
- SPARC-null mice develop region-specific, age-dependent hypersensitivity to cold, icilin, and capsaicin; axial discomfort; motor impairment; and reduced physical function. PMID: 22414871
- SPARC suppresses angiogenesis of gastric cancer by down-regulating the expression of VEGF and MMP-7 PMID: 22957090
- Stromal SPARC contributes to the detrimental fibrotic changes associated with myeloproliferation whereas its deficiency favors myeloid cell expansion. PMID: 22955913
- SPARC promotes the development of erythroid progenitors, but does not affect terminal erythroid differentiation. PMID: 22687753
- The Sparc(-/-) mice had reduced survival and fewer well-defined abdominal lesions compared with WT controls after IP injection, whereas no differences were observed in survival or abdominal lesions between Sparc(-/-) and WT mice after OT injection. PMID: 22003835
- Osteonectin may suppress prostate cancer pathogenesis. PMID: 22525512
- aberrant TGFbeta1-activation in the absence of host SPARC contributes significantly to tumor progression and SPARC, by controlling ECM deposition and maturation, can regulate TGFbeta availability and activation PMID: 22348081
- SPARC influences the shuttling of Pax6 via the TGF-beta/Smad signaling pathway. PMID: 22539874
- follicular dendritic cell networking toward Th17 responses PMID: 21962567
- Data indicate that intracellular SPARC-positive vesicles did not undergo visible degradation. PMID: 21949685
- SPARC and connective tissue growth factor appeared to be involved in the same biological pathway PMID: 21978691
- DNA methylation down-regulates SPARC expression in chronic low back pain (LBP) in pre-clinical models and in patients with chronic LBP. PMID: 21867537
- SPARC regulates collagen in the heart by modulating procollagen processing and interactions with fibroblast cell surfaces. PMID: 21666116
- These results identify hevin as a positive and SPARC as a negative regulator of synapse formation. PMID: 21788491
- SPARC deletion preserves left ventricular function at day 3 post-myocardial infarction but may be detrimental for the long-term response due to impaired fibroblast activation. PMID: 21602472
- SPARC promotes pericyte migration by diminishing TGF-beta activity and identify a novel function for endoglin in controlling pericyte behavior. PMID: 21708981
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亞細(xì)胞定位：Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane.
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蛋白家族：SPARC family
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