Pig Angiopoietin 1,ANG-1 ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬血管生成素1(ANG-1)酶聯(lián)免疫試劑盒（CSB-E08457p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ANGPT1含量。ANGPT1是一個熱門靶點。它是血管生成素家族成員，在血管形成、穩(wěn)定及內(nèi)皮細胞功能維持中有重要作用。研究機制主要圍繞其與Tie2受體相互作用，調(diào)控血管的發(fā)育、重塑等過程，為心血管疾病等治療提供潛在方向。試劑盒檢測范圍為6 ng/mL-200 ng/mL，心血管疾病機制研究、血管生成相關(guān)動物模型分析以及炎癥或組織修復過程中的分子調(diào)控探索等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

ANGPT1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit
縮寫：

ANGPT1
Uniprot No.：

Q9BDY8
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6 ng/mL-200 ng/mL
靈敏度：

6 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	95
	Range %	85-100
1:4	Average %	89
	Range %	85-101
1:8	Average %	90
	Range %	80-99

回收率：

The recovery of pig ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-105
EDTA plasma (n=4)	96	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
基因功能參考文獻：
1. Angiopoietin 1 was predominantly present in the maturing glomeruli. PMID: 20598355
2. The expression of ANGPT1 has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros. PMID: 20713984
3. Attenuates H2O2-induced stress-activated protein kinase (SEK)1/JNK phosphorylation through the PI 3-kinase/Akt pathway and inhibits the apoptosis of vascular endothelial cells induced by oxidative stress. PMID: 16000309
4. Estrogen-dependent control of Ang-1 expression plays an important role in stabilizing meningeal microvessel and maintaining healthy microvascular networks. PMID: 17496211
亞細胞定位：

Secreted.
數(shù)據(jù)庫鏈接：

KEGG: ssc:397009

STRING: 9823.ENSSSCP00000006448

UniGene: Ssc.16730