Rat Platelet Factor 4,PF-4 ELISA Kit
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中文名稱：大鼠血小板因子4(PF-4/CXCL4)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E07885r
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規(guī)格：96T/48T
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價(jià)格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠血小板因子4(PF-4/CXCL4)酶聯(lián)免疫試劑盒（CSB-E07885r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PF4含量。PF4即血小板因子4，是血小板α顆粒分泌的一種小分子蛋白。在生理上它能趨化炎癥細(xì)胞、參與凝血等。研究機(jī)制方面，其在血栓形成、腫瘤微環(huán)境調(diào)節(jié)等過程有作用，深入研究它對(duì)相關(guān)疾病治療靶點(diǎn)的開發(fā)具有重要意義。試劑盒檢測(cè)范圍為0.156 μg/mL-10 μg/mL，靈敏度為0.039 μg/mL。科研人員可將其應(yīng)用于血小板活化機(jī)制、血栓相關(guān)疾病模型或炎癥微環(huán)境等研究領(lǐng)域，為探索PF - 4/CXCL4在病理生理過程中的調(diào)控作用提供可靠工具。試劑盒兼容多種生物樣本類型，滿足實(shí)驗(yàn)室基礎(chǔ)研究及藥物開發(fā)中的靶點(diǎn)驗(yàn)證需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：0.156 μg/mL-10 μg/mL
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靈敏度：0.039 μg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cardiovascular
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 86-97 1:2 Average % 95 Range % 88-99 1:4 Average % 95 Range % 91-102 1:8 Average % 95 Range % 87-104 -
回收率：
The recovery of rat PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 83-99 EDTA plasma (n=4) 97 89-102 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
μg/ml OD1 OD2 Average Corrected 10 2.684 2.617 2.651 2.463 5 2.204 2.186 2.195 2.007 2.5 1.563 1.608 1.586 1.398 1.25 0.881 0.899 0.890 0.702 0.625 0.480 0.491 0.486 0.298 0.312 0.345 0.337 0.341 0.153 0.156 0.247 0.250 0.249 0.061 0 0.185 0.190 0.188 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Influence of brush length of PVP chains immobilized on silicon wafers on their blood compatibility Shengqiang Nie.et al,Polymers for Advanced Technologies,2017
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
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基因功能參考文獻(xiàn)：
- identified the informative Chronic Liver Allograft Dysfunction (CLAD)-associated genes; CXCL4, CXCR3, EGFR, JAK2, STAT3, and Collagen IV were associated with CLAD pathogenesis; validated that CXCL4 is upstream of these informative genes in the isolated hepatic stellate cells PMID: 28053995
- A carboxyl-terminal fragment of this protein effects a rapid histamine release from rat peritoneal mast cells. PMID: 12383857
- this study identified a novel regulatory element,TME(tandem repeat of MEIS1 binding element)in the PF4 promoter and demonstrated that MEIS1/PBX complexes bind to the TME PMID: 12609849
- PF4 promoters are transactivated by USF1 and 2 which may play an important role in megakaryocytic gene expression PMID: 15187018
- The levels of PF-4 (as a platelet marker) mRNA were significantly higher in FeCl(2)-VT (2(-DeltaDeltaCT)=7.8) than in stasis-VT (2(-DeltaDeltaCT)=4.2, p<0.05). PMID: 16797058
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亞細(xì)胞定位：Secreted.
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蛋白家族：Intercrine alpha (chemokine CxC) family
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數(shù)據(jù)庫(kù)鏈接：
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