Rat Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit
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中文名稱：大鼠可溶性CD40配體(sCD40L)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E07395r
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠可溶性CD40配體(sCD40L)酶聯(lián)免疫試劑盒（CSB-E07395r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD40LG含量。CD40LG，全稱CD40配體，是一種腫瘤壞死因子超家族成員，主要表達(dá)于T細(xì)胞表面，通過與抗原呈遞細(xì)胞上的CD40結(jié)合，參與免疫反應(yīng)和腫瘤生長調(diào)控。研究顯示，CD40LG在乳腺癌等腫瘤中表達(dá)異常，可能成為預(yù)后預(yù)測和治療的新靶點(diǎn)。試劑盒檢測范圍為1.87 ng/mL-120 ng/mL，本試劑盒適用于研究心血管疾病、慢性炎癥或免疫相關(guān)疾病模型中 sCD40L 的動(dòng)態(tài)變化，可兼容多種生物樣本類型，為探索其生物學(xué)功能及病理機(jī)制提供可靠工具。滿足科研場景下對(duì)大鼠 sCD40L 的精準(zhǔn)定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Cd40lg ELISA Kit; Cd40l ELISA Kit; Tnfsf5CD40 ligand ELISA Kit; CD40-L ELISA Kit; Tumor necrosis factor ligand superfamily member 5 ELISA Kit; CD antigen CD154) [Cleaved into: CD40 ligand ELISA Kit; membrane form; CD40 ligand ELISA Kit; soluble form ELISA Kit; sCD40L)] ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：1.87 ng/mL-120 ng/mL
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靈敏度：0.47 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 83-98 1:2 Average % 93 Range % 87-98 1:4 Average % 91 Range % 83-95 1:8 Average % 103 Range % 95-107 -
回收率：
The recovery of rat sCD40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 88 82-92 EDTA plasma (n=4) 95 90-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 120 2.487 2.688 2.588 2.437 60 1.734 1.643 1.689 1.538 30 1.049 1.106 1.078 0.927 15 0.674 0.685 0.680 0.529 7.5 0.442 0.457 0.450 0.299 3.75 0.314 0.327 0.321 0.170 1.87 0.236 0.245 0.241 0.090 0 0.150 0.151 0.151 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Higher mitochondrial potential and elevated mitochondrial respiration are associated with excessive activation of blood platelets in diabetic rats. Siewiera K. et al,Life Sci.,2016
- Long-term untreated streptozotocin-diabetes leads to increased expression and elevated activity of prostaglandin H2 synthase in blood platelets Siewiera K. et al,Platelets,2015
- Biomarkers for Bisphosphonate??elated Osteonecrosis of the Jaw Kim JW et al,Clin Implant Dent Relat Res,2015
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靶點(diǎn)詳情
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功能：Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.; Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
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基因功能參考文獻(xiàn)：
- Transient middle cerebral artery occlusion (MCAO) rats yielded an acceleration of thrombus formation that was accompanied by increased CD40 levels in serum. The brain infarction was significantly decreased in CD40 antagonist treatment group compared to MCAO model group. The mTOR/S6K signaling was activated in MACO model than that of CD40 antagonist treatment group. PMID: 29843579
- Soluble CD40 ligand directly alters glomerular permeability and may act as a circulating permeability factor in focal segmental glomerulosclerosis. PMID: 29155846
- Suggest that CD154-CD40 T-cell co-stimulation pathway is a key mechanism in kidney ischemia-reperfusion injury. PMID: 25993320
- These data suggest that placental ischemia-induced T-cell communication via the CD40L is one important mechanism leading to much of the pathophysiology of preeclampsia. PMID: 26310940
- PSB33 functions in the maintenance of photosystem II-light-harvesting complex II.[PSB33] PMID: 25511433
- Data suggest that astrocytes activated by CD40-CD40L in co-culture induce inflammatory cytokine production, and secreted cytokines re-activate astrocytes via Jak/STAT1701 pathways, and release more cytokines that contribute to the development of EAE. PMID: 21410936
- Monoclonal antibodies are immunosuppressive in pig to rat islet xenograft. PMID: 15665763
- B cells play a cooperative role via CD40L-CD40 interaction in T cell-mediated experimental autoimmune neuritis of Lewis rats PMID: 17188497
- HO-1 mitigated CD40L transcription in the xenograft and recipient spleen. PMID: 17976119
- Generated and characterized a novel anti-rat CD40L antibody with inhibitory activities. PMID: 18384807
- Adenovirus-mediated Cd40lg gene therapy induced both humoral and cellular immunity against rat model of hepatocellular carcinoma. PMID: 19016771
- Primary tumor cells obtained from MNU-induced mammary carcinomas show immune heterogeneity which can be modulated by low-efficiency transfection of CD40L gene. PMID: 19164932
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亞細(xì)胞定位：Cell membrane; Single-pass type II membrane protein. Cell surface.; [CD40 ligand, soluble form]: Secreted.
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蛋白家族：Tumor necrosis factor family
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