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中文名稱：綿羊腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒
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貨號：CSB-E13853Sh
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規格：96T/48T
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價格：￥4200/￥3000
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其他：
產品詳情
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產品描述：綿羊腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒（CSB-E13853Sh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF含量。TNF（腫瘤壞死因子）是一種多功能促炎細胞因子，最初因能引起腫瘤組織壞死而得名。其超家族包括19個配體和29個受體，參與宿主防御、炎癥、細胞凋亡等生理過程。TNF通過與其受體結合，激活下游信號通路，調節細胞存活、凋亡和炎癥反應。試劑盒檢測范圍為3.125 pg/mL-50 pg/mL，TNF-α的動態監測對綿羊感染性疾病模型、疫苗研發及免疫機制研究具有重要價值；可高效支持動物醫學研究中炎癥相關疾病模型的建立、病原體感染后免疫應答評估以及抗炎藥物作用機制的體外實驗，為畜牧科研中的免疫調控研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Ovis aries (Sheep)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：3.125 pg/mL-50 pg/mL
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靈敏度：3.125 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Immunology
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of sheep TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 84 Range % 80-88 1:2 Average % 95 Range % 90-99 1:4 Average % 90 Range % 85-94 1:8 Average % 102 Range % 98-109 -
回收率：
The recovery of sheep TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 84-95 EDTA plasma (n=4) 96 90-101 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 50 0.161 0.165 0.163 25 0.308 0.324 0.316 12.5 0.551 0.512 0.532 6.25 1.012 0.982 0.997 3.125 2.054 2.011 2.033 -
數據處理：
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貨期：3-5 working days
引用文獻
- Physiological Responses, Oxidative Status and Productive Performance of Barki Sheep fed Dietary Addition with Quinoa Seeds and Exposed to Solar Radiation WA Fouda,/,2024
- Impact of Vitamin D Supplement on Reproductive Performance in Pre-mating Ewes: Presumptive Regulatory Role of Some Inflammatory Cytokines and Serum Biomarkers GA El-Amrawi,Journal of Veterinary Sciences,2023
- Immunological response of lame sheep to Clinical Interdigital Dermatitis and Footrot: Procalcitonin, Acute phase proteins, and Pro-inflammatory Cytokines W El-Deeb,Comparative immunology, microbiology and infectious diseases,2022
- Characterization of immunological, biochemical and inflammatory response of clinical and subclinical endometritis in ewes in the subtropics N Nasreldin,Animal Reproduction Science,2020
- Peripheral and placental immune responses in sheep after experimental infection with Toxoplasma gondii at the three terms of gestation Castano P, et al,Veterinary Research,2019
- Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle Herman A P, et al,International Journal of Molecular Sciences,2019
- Acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep, goats and she-camels with Coxiella burnetii infection-induced abortion El-Deeb W, et al,Comparative Immunology, Microbiology and Infectious Diseases,2019
- Neostigmine Attenuates Proinflammatory Cytokine Expression in Preoptic Area but Not Choroid Plexus during Lipopolysaccharide-Induced Systemic Inflammation Andrzej P. Herman .et al,Mediators of Inflammation,2018
- The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis El-Deeb W M,.et al, PeerJ,2016
- Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis El-Deeb W M.et al, Comparative Clinical Pathology,,2015
- The proteome of Hypobaric Induced Hypoxic Lung: Insights from Temporal Proteomic Profiling for Biomarker Discovery Ahmad Y. et al,Sci Rep,2015
產品評價
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靶點詳情
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功能：Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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基因功能參考文獻：
- The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420
- The p38 MAPK inhibitor decreased (P < 0.01) gene expression of TNFa but its effect was not observed at the level of TNFa protein synthesis PMID: 24995301
- TNF-alpha gene expression in multifidus was greater on both sides in the disc lesion animals than in the muscle of control animals. PMID: 24718080
- Injection of peptidoglycan-polysaccharide resulted in fever, increased concentrations of TNF-alpha on the day of and the day after challenge, and decreased concentrations of progesterone on days 14 and 21. PMID: 20603027
- These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
- plasma IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes PMID: 17221363
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亞細胞定位：Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
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蛋白家族：Tumor necrosis factor family
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數據庫鏈接：
KEGG: oas:443540
UniGene: Oar.455
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