Recombinant Human Neuropilin-1 (NRP1) (Active)

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評(píng)價(jià) (1)

中文名稱：

人NRP1重組蛋白
貨號(hào)：

CSB-MP016091HU
說明書：

MSDS Datasheet
規(guī)格：

￥696
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized human VEGF165 (CSB-MP025833HU(F4)) at 2 μg/ml can bind human NRP1, the EC₅₀ is 22.68-34.55 ng/ml. Biological Activity Assay
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
內(nèi)毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized human VEGF165 (CSB-MP025833HU(F4)) at 2 μg/ml can bind human NRP1, the EC₅₀ is 22.68-34.55 ng/ml.
基因名：

NRP1
Uniprot No.：

O14786
別名：

(Vascular endothelial cell growth factor 165 receptor)(CD304)
分子結(jié)構(gòu)：
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Full Length of Mature Protein of Isoform 2
來源：

Mammalian cell
分子量：

98.8 kDa
表達(dá)區(qū)域：

22-644aa
氨基酸序列

FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK
蛋白標(biāo)簽：

C-terminal hFc1-tagged
產(chǎn)品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

The gene fragment coding for amino acids 22-644 of the human Neuropilin-1 (NRP1) isoform 2 is expressed in mammalian cells with a human Fc tag at the C-terminus. The product is the recombinant human NRP1 protein. This NRP1 protein has been demonstrated to be active. In the functional ELISA, immobilized human VEGF165 can bind to this NRP1 protein. The EC₅₀ is 22.68-34.55 ng/ml. It reaches up to 95% in purity. In the SDS-PAGE, this protein migrated to the band of molecular weight about 116 kDa on the gel. Its endotoxin is less than 1.0 EU/ug determined by the LAL method. This recombinant NRP1 protein is in stock.

NRP1, a member of a family of signaling and catalytic proteins, exists as two isoforms: a secreted form (sNRP1) and a transmembrane form. sNRP1 has been known to suppress cell-associated NRP-1 function in cervical cancer. And patients with cervical cancer have significantly higher circulating sNRP-1 levels. The transmembrane form of NRP1 plays important roles in the development of the nervous and cardiovascular system, tumorgenesis, and viral entry through interaction with VEGF, Sema3A, TGF-β, plexins, and integrins. Studies have demonstrated that NRP1 facilitates SARS-CoV-2 entry into host cells and significantly potentiates SARS-CoV-2 infectivity in vitro. NRP1 thus may potentially provide a therapeutic target for COVID-19.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

平均分:

5.0分 - 1 個(gè)評(píng)價(jià)

實(shí)驗(yàn)應(yīng)用：ELISA標(biāo)準(zhǔn)品

產(chǎn)品評(píng)價(jià)：收到產(chǎn)品后進(jìn)行SDS-PAGE檢測(cè)，蛋白純度高，后續(xù)進(jìn)行免疫吸附實(shí)驗(yàn)，實(shí)驗(yàn)結(jié)果很不錯(cuò)，曲線線性好，包被低濃度就有較好的結(jié)合，從一定程度上節(jié)約了成本。蛋白穩(wěn)定，重復(fù)性好！

By 賀老師

靶點(diǎn)詳情

功能：

Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR.; Binds VEGF-165 and may inhibit its binding to cells. May induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.; (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection.
基因功能參考文獻(xiàn)：
1. HLA-G, NRP1, and PD-1, may be involved in the immune response in psoriatic patients PMID: 29790686
2. miR-320/NRP-1 axis contributes to the growth and metastasis of cholangiocarcinoma cells. PMID: 28618167
3. we demonstrated that HS3ST3B enhances the proliferation and survival of breast cancer MDA-MB-231 cells, via a mechanism that is dependent on Nrp1. PMID: 30360368
4. The serum NRP1 was significantly associated with AFP, gamma-GT, Alb, bile acid, ALT, AST, ALP and pre-Alb. NRP1 is a novel tumor marker in HCC. PMID: 29966621
5. lnc00152 was aberrantly overexpressed in colorectal tumors and cancer cells and that lnc00152 was modulated by miRNA206. lnc00152 overexpression promoted epithelial-mesenchymal transition and increased neuropilin1 expression in the colorectal cancer cells. PMID: 29956750
6. the results presented herein indicate that NRP1 exerts tumor suppressive effects in NB, at least in part by regulating the expression of beta1 integrin PMID: 29750423
7. NRP1 may be important in the etiology of menstrual migraine. PMID: 29671086
8. clinical samples from apical periodontitis patients were obtained to analyse the expression of Sema3A/Nrp1. These results indicated that the bone destruction level expanded from days 7 to 35 PMID: 29457037
9. Study demonstrated that NRP1+ cells possess tumor-initiating cells (TIC) properties, including the preferential expression of stemness markers, higher clonogenic and self-renewal potential, and increased cell migration capacity. These findings support an important role for NRP1 in the biology of lung cancer TICs. PMID: 29138851
10. Authors demonstrated that when VEGFR2 was inhibited, NRP-1 appeared to regulate RAD51 expression through the VEGFR2-independent ABL-1 pathway, consequently regulating radiation sensitivity. In addition, the combined inhibition of VEGFR2 and NRP-1 appears to sensitize cancer cells to radiation. PMID: 29777301
11. NRP1, as a promoter of odontoblast differentiation, regulates dental pulp stem cells via the classical Wnt/beta-catenin pathway. PMID: 28910136
12. The new observation that shows strong NP-1 immunoreactivity of these particles, and decreased NP1 expression in syncytiotrophoblast of preeclampsia (PET) placentas in comparison to the control group, may imply a role of NP-1 in PET. PMID: 26937865
13. rs2228638 of NRP1 may have a role in tetralogy of Fallot risk in European and Chinese Han populations PMID: 29432830
14. Data show that neuropilin 1 (NRP1) binds extracellular AGO2 (carrying miRNA or not), and internalizes AGO2/miRNA complexes. PMID: 27486976
15. Data suggest neuropilin-1 (NRP-1) as a therapeutic target to reduce tumor fibrosis and pancreatic ductal adenocarcinoma (PDAC) progression. PMID: 27542226
16. Results suggest that targeting the transmembrane domains (TMD) of neuropilin-1 (NRP1) in breast cancer is a potent new strategy to fight against breast cancer and related metastasis. PMID: 27351129
17. By blocking neuropilin-1 receptor (NRP-1) in tumor-bearing mice, luciferase activity in tumors delivered by HK polyplexes was reduced by 96%, whereas activity in normal tissues was minimally reduced. PMID: 27257039
18. High NRP1 expression is associated with metastasis in triple-negative breast cancer. PMID: 26967387
19. Overexpression of Neuropilin 1 incresed in breast cancer stem cells formation and breast cancer stem cells-induced tube formation and knockdown of Neuropilin 1 reduced CSCs-related traits. PMID: 29179185
20. Nrp1 mediates the migratory behavior of mesangial cells. PMID: 28835419
21. this study identified the NRP1 cytoplasmic domain as essential for acute vascular hyperpermeability induced by different NRP1 ligands: a ligand- blocking antibody against NRP1 and a CendR peptide. PMID: 27117252
22. Data indicate that miR-206 inhibits neuropilin-1 (NRP1) and SMAD2 gene expression by directly binding to their 3'-UTRs. PMID: 27014911
23. Results show that Nrp1 plays a critical role in balancing responsiveness to VEGF-A versus TGFbeta to regulate glioblastoma growth, progression, and recurrence after anti-vascular therapy. PMID: 28938007
24. Data provide evidence that NRP1 functions to enhance the metastatic potential of prostate tumors. PMID: 28092670
25. NRP1 is an important niche component PMID: 27676403
26. our data suggest a novel molecular mechanism by which tMUC1 may modulate NRP1-dependent VEGFR signaling in PDA cells. PMID: 26804176
27. Our findings identify aberrant active integrin b1-driven Src-Akt hyperactivation as a primary resistance mechanism to cetuximab in PDAC cells and offer an effective therapeutic strategy to overcome this resistance using an EGFR and NRP1 dual targeting antibody PMID: 27797376
28. VEGF-A acts via interaction with NRP-1 to trigger intracellular events leading to ECS cell survival and formation of aggressive, invasive and highly vascularized tumors. PMID: 26804163
29. These data identify a new molecular mechanism of brain microvascular endothelial inflammatory response through NRP1-IFNgamma crosstalk. PMID: 27591257
30. This study indicates that capsaicin application results in significant loss of epidermal NRP-1 receptor expression, whereas diabetic subjects presenting small fiber neuropathy show full epidermal NRP-1 expression in contrast to the basal expression pattern seen in healthy controls PMID: 27598321
31. Study show that a high percentage of intratumoral NRP1(+) Tregs correlates with poor prognosis in melanoma and head and neck squamous cell carcinoma. PMID: 28552348
32. Study revels the structural description of the neuropilin-1 ectopic region by reporting the crystal structure of its MAM domain. The domain adopts a jellyroll fold that is stabilized by a Ca2+ ion while forming a molecular surface that is distinct from its structural homologs. PMID: 27720589
33. Data suggest that neuropilin-1 (NRP1) functions as a receptor for neutrophil elastase (NE), mediates uptake/absorption of NE, and facilitates cross-presentation of PR1 (a peptide fragment of NE) in breast cancer cells. PMID: 28468826
34. This report is the first study characterizing the specific functions of NRP1-Delta7, a glycosaminoglycan-defective neuropilin-1 splice variant that displays anti-tumorigenic properties in vitro and in vivo. PMID: 27798666
35. this study shows that NRP1 can act as a co-receptor for PDGF-D-PDGFRbeta signaling and is possibly implicated in intercellular communication in the vascular wall PMID: 28254885
36. This review provides a general overview of current knowledge of the signalling pathways that are modulated by NRP1, with particular focus on neuronal and vascular roles in the brain and retina. PMID: 26923176
37. Curcumin-loaded nanoliposomes linked to homing peptides for integrin targeting and neuropilin-1-mediated internalization reduced viability of breast cancer cells. PMID: 27937055
38. Upregulated expression of NRP1 is associated with glioma. PMID: 27481513
39. Studies suggest that the activation of NRP-1 by PlGF directly contributes to tumour aggressiveness and to melanoma escape from anti-VEGF-A therapies. PMID: 26846845
40. REVIEW: Nrp1 functions in the vasculature is critical for the development of targeted therapeutics for cancer and vascular diseases such as atherosclerosis and retinopathies. PMID: 26849476
41. High Expression of Neuropilin-1 Associates with Hepatocellular Carcinoma. PMID: 26563279
42. Increased NRP-1 expression is associated with metastatic endometrial and lung cancers. PMID: 26701889
43. miR-152 suppression in NSCLC cells might promote neuropilin-1 mediated cancer metastasis. PMID: 26823738
44. NRP-1 was found to be overexpressed in gastric cancer (GC) tissues, and its expression correlates with the clinical staging, tumor differentiation and pathological types of gastric cancer. PMID: 26795388
45. NRP1 was targeted by miR130a and miR130b at the binding site of chromosome 10: 334668643466870, which was involved in the axon guidance signaling pathway. PMID: 26573160
46. Semaphorin-3a, neuropilin-1 and plexin-A1 are axonal guidance molecules that have been recently implicated in regulating bone metabolism. PMID: 26602825
47. neuropilin-1 is regulated in the oral epithelium and is selectively up-regulated during epithelial dysplasia PMID: 26877262
48. NRP1 over-expression in miR-365 expressing cells could rescue invasion and growth defects of miR-365. In addition, miR-365 expression inversely correlated with NRP1 protein levels in malignant melanoma PMID: 26406949
49. Data show that binding of pleiotrophin (PTN) to neuropilin-1 (NRP-1) stimulated the internalization and recycling of NRP-1 at the cell surface. PMID: 26408254
50. Study found significant down-regulation of placental NRP-1 expression in fetal growth restriction pregnancies complicated with absent end-diastolic flow in the umbilical artery. PMID: 26409917
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亞細(xì)胞定位：

[Isoform 2]: Secreted.; Mitochondrion membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Cytoplasm.
蛋白家族：

Neuropilin family
組織特異性：

[Isoform 1]: The expression of isoforms 1 and 2 does not seem to overlap. Expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and
數(shù)據(jù)庫(kù)鏈接：

HGNC: 8004

OMIM: 602069

KEGG: hsa:8829

STRING: 9606.ENSP00000265371

UniGene: Hs.131704