Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (R203K,G204R) (Active)

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品名簡(jiǎn)稱：

Recombinant SARS-CoV-2 N protein (R203K,G204R) (Active)
貨號(hào)：

CSB-EP3325GMY(M12)
說(shuō)明書：

MSDS Datasheet
規(guī)格：

￥1140
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M12)) at 2 μg/ml can bind N Mouse Monoclonal Antibody(CSB-MA33255A2m), the EC₅₀ of N Mouse Monoclonal Antibody is 6.775-13.04 ng/ml. Biological Activity Assay

Activity
Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M12)) at 2 μg/ml can bind N Recombinant Antibody(CSB-RA33255A1GMY), the EC₅₀ of N Recombinant Antibody is 4.664-8.256 ng/ml. Biological Activity Assay
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 96% as determined by SDS-PAGE.
生物活性：

①M(fèi)easured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M12)) at 2 μg/ml can bind N Mouse Monoclonal Antibody(CSB-MA33255A2m), the EC₅₀ of N Mouse Monoclonal Antibody is 6.775-13.04 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M12)) at 2 μg/ml can bind N Recombinant Antibody(CSB-RA33255A1GMY), the EC₅₀ of N Recombinant Antibody is 4.664-8.256 ng/ml.
基因名：

N
Uniprot No.：

P0DTC9
別名：

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
種屬：

Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
蛋白長(zhǎng)度：

Full Length
來(lái)源：

E.coli
分子量：

49.8 kDa
表達(dá)區(qū)域：

1-419aa(R203K,G204R)
氨基酸序列

MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
亞細(xì)胞定位：

Virion. Host cytoplasm.
蛋白家族：

Betacoronavirus nucleocapsid protein family