Recombinant Human Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), partial (Active)

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評價 (2)

中文名稱：

人TNFR1重組蛋白
貨號：

CSB-MP023977HU1
說明書：

MSDS Datasheet
規(guī)格：

￥588
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized TNF-α (CSB-YP023955HU) at 5 μg/ml can bind human TNFR1, the EC₅₀ is 7.799-10.90 ng/ml. Biological Activity Assay

Activity
Measured by its binding ability in a functional ELISA. Immobilized LTA(CSB-MP013218HU) at 5 μg/ml can bind human TNFR1, the EC₅₀ is 4.409-6.797 ng/ml. Biological Activity Assay
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

①Measured by its binding ability in a functional ELISA. Immobilized TNF-α (CSB-YP023955HU) at 5 μg/ml can bind human TNFR1, the EC₅₀ is 7.799-10.90 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized LTA(CSB-MP013218HU) at 5 μg/ml can bind human TNFR1, the EC₅₀ is 4.409-6.797 ng/ml.
基因名：

TNFR1
Uniprot No.：

P19438
別名：

(TNF-R1)(Tumor necrosis factor receptor type I)(TNF-RI)(TNFR-I)(p55)(p60)(CD120a)(TNFAR)(TNFR1)
分子結構：
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

50.1 kDa
表達區(qū)域：

22-211aa
氨基酸序列

IYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTT
蛋白標簽：

C-terminal hFc1-tagged
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

A construct encoding COOH-terminally hFc-tagged human TNFRSF1A was expressed in mammalian cells. The resulting protein is the recombinant TNFRSF1A protein containing amino acid sequence 22-211 of human TNFRSF1A. The purity of this TNFRSF1A protein is greater than 94% determined by SDS-PAGE. A molecular mass band of 55 kDa was presented on the gel under reducing conditions. It contains low levels of endotoxin, less than 1.0 EU/ug determined by the LAL method. And this TNFRSF1A protein has also been tested as an active protein through the functional ELISA. This TNFRSF1A protein can bind to TNF-α or LTA, with the EC₅₀ of 7.799-10.90 ng/ml or 4.409-6.797 ng/ml. It is available now.

TNFRSF1A, also called TNFR1, is a death receptor (DR) and also a central mediator in the signal transduction of the inflammatory pathway. The treatment of autoimmune diseases such as rheumatoid arthritis has been targeted to inhibit TNFR1, while activation of TNFR1 is critical for the treatment of immunodeficiency diseases such as HIV and neurodegenerative diseases such as Alzheimer's disease.
Datasheet & COA：

Please contact us to get it.

產品評價

平均分:

5.0分 - 2 個評價

實驗應用：體外酶活性檢測

產品評價：最開始，我購買了小規(guī)格的human TNFR1蛋白，進行實驗之后發(fā)現效果不錯，活性好，又繼續(xù)購買了大規(guī)格的蛋白產品，用于后續(xù)實驗，實驗結果也很穩(wěn)定，基本無批間差。采購方便，到貨快，價格也很優(yōu)惠！

By 魯老師

實驗應用：藥物相關研究

產品評價： TNFRSF1A純度高，內毒素低，有完整的生物活性，后續(xù)用于抗體篩選，通過流式篩選與其結合的分子，結果不錯，篩到了符合預期的抗體。

By 朱老師

靶點詳情

功能：

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
基因功能參考文獻：
1. Elevated TNFRs levels were associated with the risk of cardiovascular and/or all-cause mortality independent of all relevant covariates in patients undergoing haemodialysis PMID: 28256549
2. It has been demonstrated that the molecular genetic marker +36G TNFR1 (OR=1,25) is involved in the formation Essential Hypertension in individuals with Metabolic Syndrome. PMID: 30289218
3. Due to the fact that the mutation was not inherited from the parents, it was likely that R426L was a de novo and novel mutation in the TNFRSF1A gene, which can trigger TRAPS or TRAPS-like symptoms. PMID: 27793577
4. this study evaluated TNFR1 -609G/T polymorphisms association with RA susceptibility in a sample of Mexican patients. Our results suggest that the TNFR1 -609G/T polymorphisms are not associated with RA susceptibility in a sample of Mexican patients. PMID: 29404828
5. Polymorphisms in the TNFR1 gene may have an impact on the symptomatology of schizophrenia in men. rs4149577 and rs1860545 SNPs were associated with the intensity of the Positive and Negative Syndrome Scale (PANSS) excitement symptoms in men, which may contribute to the risk of violent behavior. PMID: 29317797
6. Polymorphism of Promoter Region of TNFRSF1A Gene is associated with Radiotherapy Induced Oral Mucositis in Head and Neck Cancer. PMID: 28401452
7. five single nucleotide polymorphisms in the TNFRSF1A gene are not associated with autoimmune thyroid diseases in the Chinese Han population, but rs4149570 shows a weak association with Hashimoto's thyroiditis after adjusting for gender and age. PMID: 29401539
8. Genotype rs767455 was associated with the susceptibility of ankylosing spondylitis(AS), G allele of rs767455 exhibited an association with the risk of developing AS. Only rs1061622 was significantly associated with long-term efficacy of etanercept. The results suggest that TNFRSF1A and TNFRSF1B polymorphisms were associated with susceptibility, severity, and the long-term therapeutic efficacy of etanercept of AS patients. PMID: 30075559
9. RACK1 associates with MOAP-1 via electrostatic associations similar to those observed between MOAP-1/RASSF1A and MOAP-1/TNF-R1. These events illustrate the complex nature of MOAP-1 regulation and characterizes the important role of the scaffolding protein, RACK1, in influencing MOAP-1 biology. PMID: 29470995
10. serum level did not decrease significantly after tonsillectomy with steroid pulse therapy in IgA nephropathy PMID: 28389814
11. The TNFRSF1A c.625+10 G allele was associated with late response to anti-TNFalpha therapy but TNFRSF1A gene SNPs is not associated with spondyloarthritis. PMID: 29579081
12. TNFR1 is associated longitudinally with kidney function decline but not with myocardial infarct, heart failure, or mortality risk after adjustment PMID: 28601698
13. One third of our childhood MS patients had a heterozygous mutation in the TNFRSF1A and/or MEFV gene. This proportion by far exceeds the number of mutations expected and was higher than in adult MS patients, suggesting that these mutations might contribute to the pathogenesis of childhood MS. PMID: 28927886
14. Studied the association of NLR family pyrin domain containing 3 (NLRP3) and tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) polymorphisms and haplotypes in patients with ankylosing spondylitis (AS) and treatment response to etanercept. PMID: 28116820
15. Investigations of underlying molecular mechanisms of TNFR1 signaling showed that PDF affects TNFR1 signaling at the proapoptotic signaling pathway by upregulation of IkappaBalpha and downregulation of cFLIPL. PMID: 28096440
16. Case Report: tumor necrosis factor receptor-associated periodic syndrome due to the R92Q TNFRSF1A variant associated with recurrent pericarditis and cardiac tamponade. PMID: 27990755
17. Serum TNFR1 is a biomarker for patients with chronic kidney disease. PMID: 28667032
18. This study demonstrated that TNFR1 expression levels are related to major depressive disorder and conjunctly mediate the effect of childhood maltreatment history on the risk of developing major depressive disorder. PMID: 28384542
19. SNP (36A>G) is strong risk factor for odontogenic keratocystic tumor PMID: 28199753
20. Data suggest that Fas and TNFR1 are involved in glaucoma mechanisms in cornea; pro-apoptotic effect of anti-glaucoma medication clonidine on corneal epithelial cells triggers Fas/TNFR1-mediated, mitochondria-dependent signaling pathway. (Fas = Fas cell surface death receptor ; TNFR1 = TNF receptor superfamily member 1A) PMID: 28115640
21. These results indicate that TNFRI-Fc and hHO-1 overexpression may apparently induce free iron in the liver and exert oxidative stress by enhancing reactive oxygen species production and block normal postneonatal liver metabolism. PMID: 28503569
22. Data suggest that elevated serum levels of soluble TNF receptors, especially sTNFR1, are associated with loss of kidney function in Hispanic patients with diabetes type 2 in Colombia. PMID: 27068267
23. Case Report: heterozygous missense variants in TNFRSF1A identified in family members with familial Mediterranean fever. PMID: 29148404
24. Case Report: autoinflammatory syndrome with relapsing aseptic neutrophilic meningitis and chronic myelitis associated with MEFV/TNFRSF1A mutations. PMID: 28134085
25. In this article we will review the role of ubiquitination and proteolysis in these diverse events focusing on our own contributions to the lysosomal apoptotic pathway linked to the subcellular compartmentalization of TNF-R1 PMID: 28765050
26. Coadministration of either ATROSAB or EHD2-scTNFR2 into the magnocellular nucleus basalis significantly protected cholinergic neurons and their cortical projections against cell death, and reverted the neurodegeneration-associated memory impairment in a passive avoidance paradigm. Simultaneous blocking of TNFR1 and TNFR2 signaling, however, abrogated the therapeutic effect PMID: 27791020
27. Data show that interleukin-2 receptor alpha, tumor necrosis factor receptor 1, serum STimulation-2 (IL1RL1 gene product), and regenerating islet-derived 3-alpha were significantly associated with non-relapse mortality. PMID: 28126963
28. Report of a severe case of TRAPS associated with a novel mutation, Thr90Pro, in the TNFRSF1A gene in an infant and several family members. PMID: 28427379
29. atopic dermatits patients had increased TNFR1 expression on immune cells PMID: 29212072
30. Elevated level of soluble tumor necrosis factor receptors 1 and lower level of leptin are associated with higher developmental outcomes in infants between the ages of 6 and 24 months. PMID: 28238825
31. The highest levels of TNFR1 are independently associated with progression of renal disease and death in type 2 diabetic nephropathy. PMID: 27003829
32. High plasma levels of TNFR1 and TNFR2 were associated with incident intracerebral hemorrhage. PMID: 28830973
33. Renal clear cell carcinoma cells cells express increased amounts of RIPK1 and RIPK3 and are poised to undergo necroptosis in response to TNFR1 signaling. PMID: 27362805
34. TRIM28 acts as a central factor in controlling endothelial inflammatory responses and angiogenic activities by retaining expression of TNFR-1 and -2 and VEGF receptor 2 in endothelial cells PMID: 28159803
35. a specific link between the penetrance of the TNFRSF1A mutation and the observed T cell phenotype, is reported. PMID: 26598380
36. Collectively, this study provides more insights into RELT expression, RELT family member function, and the mechanism of RELT-induced death. PMID: 28688764
37. Burkholderia cenocepacia BcaA binds to tumor necrosis factor receptor 1. PMID: 27684048
38. TNFRSF1A variants were identified in 10 tumor necrosis factor receptor-associated periodic syndrome patients from 10 independent families. The T61I variant was found in patients, while the V136M and S321I variants were found in 1 patient each. All the patients were heterozygous for the variants. Among the healthy controls, 7 of 363 individuals were heterozygous for the T61I variant. PMID: 27332769
39. Voxel-based morphometry was used to analyse the associations between TNFRSF1A (rs4149576 and rs4149577) and grey matter structure. Highly significant genotypic associations with striatal volume but not the hippocampus were seen. Specifically, for rs4149576, G homozygotes were associated with reduced caudate nucleus volumes relative to A homozygotes and heterozygotes. Reduced caudate volumes were observed in C homozygotes PMID: 27528091
40. Circulating TNFR1 and 2 were shown to be associated with cardiovascular disease, independent of age, sex, inflammatory markers, and other cardiovascular disease risk factors, in the chronic kidney disease patients PMID: 28489742
41. Infection with C. trachomatis disturbs TNFR1 signalling specifically at the level of receptor internalization. PMID: 27062399
42. Data suggest that TRAF2 (TNF receptor-associated factor 2) negatively regulates (1) TNFR1- (tumor necrosis factor binding protein 1)-induced apoptosis, (2) TNFR2- (tumor necrosis factor receptor type 2)-induced non-canonical NFkappaB signaling, and (3) TNF- (tumor necrosis factor)-induced necroptosis. [REVIEW] PMID: 26993379
43. Our analysis showed that the TNFR1 rs2234649 polymorphism does not increase ankylosing spondylitis risk. In conclusion, the TNFR1 gene polymorphism tested does not appear to be useful for assessing predisposition to ankylosing spondylitis. PMID: 28363009
44. elevated serum level associated with heart failure risk in type 2 diabetes mellitus patients PMID: 28367848
45. The data demonstrate a novel and unexpected function of BIG1 that regulates TNFR1 signaling by targeting TRAF2. PMID: 27834853
46. Data indicate that plasma concentrations of TNFR1 and TNFR2 are elevated in pediatric lupus nephritis. PMID: 26854079
47. TNFR1 is the major pro-inflammatory mediator of TNF-alpha in fibroblast-like synoviocytes (FLS), whereas TNFR2, may act as an immunosuppressor in FLS for the prevention of overwhelming inflammatory reactions. PMID: 28150360
48. Results suggest that miR-29a is an important regulator of tumor necrosis factor receptor 1 expression in breast cancer and functions as a tumor suppressor by targeting tumor necrosis factor receptor 1 to influence the growth of MCF-7 cell. PMID: 28222663
49. Serum sTNFR1 and sTNFR2 are associated with obese girls but not obese boys, thereby suggesting that serum sTNFRs in early childhood obesity may be sex related. PMID: 27040725
50. SCCAg, CYFRA 21.1, IL-6, VEGF and sTNF receptors have roles in squamous cell cervical cancer PMID: 26289850
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相關疾病：

Familial hibernian fever (FHF); Multiple sclerosis 5 (MS5)
亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Secreted. Note=A secreted form is produced through proteolytic processing.; [Isoform 4]: Secreted. Note=Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
數據庫鏈接：

HGNC: 11916

OMIM: 142680

KEGG: hsa:7132

STRING: 9606.ENSP00000162749

UniGene: Hs.279594