Phospho-LCP2 (Y128) Antibody

說明書

中文名稱：

磷酸化-LCP2 (Y128)兔多克隆抗體
貨號：

CSB-PA009803
規格：

￥1090
圖片：
Western Blot analysis of K562 cells using Phospho-SLP-76 (Y128) Polyclonal Antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q13094
基因名：

LCP2
別名：

76 kDa tyrosine phosphoprotein antibody; CG8697 antibody; LCP 2 antibody; LCP2 antibody; LCP2_HUMAN antibody; Lymphocyte cytosolic protein 2 antibody; SH2 domain containing leukocyte protein 76 KD antibody; SH2 domain containing leukocyte protein of 76kD antibody; SH2 domain containing leukocyte protein of 76kDa antibody; SH2 domain-containing leukocyte protein of 76 kDa antibody; SLP 76 antibody; SLP 76 tyrosine phosphoprotein antibody; SLP-76 antibody; SLP-76 tyrosine phosphoprotein antibody; SLP76 antibody; SLP76 tyrosine phosphoprotein antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthesized peptide derived from Human SLP-76 around the phosphorylation site of Y128.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, IHC, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:5000

Protocols：

Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Involved in T-cell antigen receptor mediated signaling.
基因功能參考文獻：
1. These data are consistent with a model in which bivalent recruitment of a GADS/SLP-76 complex is required for costimulation by CD6. PMID: 28289074
2. LAT and SLP-76 are randomly dispersed throughout the clusters that form upon T cell receptor engagement. PMID: 27875277
3. SLP76 is ectopically expressed in chronic lymphocytic leukemia cells where it plays a role in B-cell receptor signaling. PMID: 27443285
4. findings identify ACK1 as a novel SLP-76-associated protein-tyrosine kinase that modulates early activation events in T cells. PMID: 28188290
5. Data strongly suggest that chemokine-stimulated associations between Vav1, SLP-76, and ADAP facilitate Rac1 activation and alpha4beta1-mediated adhesion, whereas Pyk2 opposes this adhesion by limiting Rac1 activation. PMID: 26202465
6. immune cell adaptor SLP-76 binds directly to SUMO-RanGAP1 of cytoplasmic fibrils of the nuclear pore complex, and this interaction is needed for optimal NFATc1 and NF-kappaB p65 nuclear entry in T cells PMID: 26321253
7. SLP-76 N-terminal tyrosine residues regulate a dynamic signaling equilibrium involving feedback of proximal T-cell receptor signaling PMID: 25316710
8. analysis of a costimulatory mechanism by which CXCL12 and antigen converge at SLP-76 microcluster formation to enhance T cell responses PMID: 23901140
9. Multipoint binding of SLP-76 to ADAP facilitates the assembly of SLP-76 microclusters. PMID: 23979596
10. Data indicate a role for the SAM domain in mediating SLP-76 self-association for T-cell function. PMID: 23935094
11. Unique modes of regulation of positive and negative feedback pathways in T cells by SLP-76. PMID: 23071622
12. These findings reveal a novel role for SLP-76 in CXCR4-mediated T lymphocyte trafficking. PMID: 22806433
13. a novel regulation mechanism of SLP-76 by ubiquitination and proteasomal degradation of activated SLP-76, which is mediated by Ser-376 phosphorylation, leading to down-regulation of TCR signaling. PMID: 22902619
14. Complementary phosphorylation sites in the adaptor protein SLP-76 promote synergistic activation of natural killer cells. PMID: 22786724
15. Nef employs a dual mechanism to disturb early TCR signaling by limiting the communication between LAT and SLP-76 PMID: 22802418
16. both T cell activation and the association between SLP-76 and Nck. After T cell receptor stimulation, SLP-76 was phosphorylated, which enabled the binding of Nck. PMID: 22534133
17. our studies demonstrate a novel role for the adaptor molecule SLP-76 in regulating HIV-1 infection in T cells PMID: 22323535
18. Combining regulated deletion of endogenous SLP-76 with transgenic expression of a SLP-76 SH2 domain mutant demonstrates that the SLP-76 SH2 domain is required for peripheral T cell activation and positive selection of thymocytes. PMID: 21949020
19. The spatial correlation between kinase ZAP70 and adaptor SLP76 microclusters (MC) at the cell periphery and the effects of F-actin on MC assembly, were analysed. PMID: 21887278
20. findings demonstrate the critical role of SLP-76-mediated signaling in initiating T-cell-directed immune responses both in vitro and in vivo PMID: 21469089
21. LAT recruits Src homology 2 domain-containing leukocyte 76 kDa protein (SLP-76) following T-cell receptor ligation and membrane translocation of Akt and phosphatidylinositol 3-kinase (PI3K)phosphorylation in Jurkat cells, activating Akt signaling. PMID: 21282515
22. Results define the composition, stoichiometry and specificity of interactions in the SLP-76, Nck and VAV1 complex, which is crucial for regulation of the actin machinery after T-cell activation. PMID: 20562827
23. findings reconfigure the TCR signaling pathway by showing SLP-76 back-regulation of ZAP-70, an event that could ensure that signaling components are in balance for optimal T cell activation PMID: 20534575
24. The results show that Bcr-Abl regulates the actin cytoskeleton and non-apoptotic membrane blebbing via a GADS/Slp-76/Nck1 adaptor protein pathway. PMID: 20079431
25. Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells (SLP-76) PMID: 12084069
26. SLP-76 is essential for NF-kappa B activation and lipid raft translocation of protein kinase C theta and the I kappa B kinase complex. PMID: 12496421
27. SLP-76 is required for intracellular calcium ion mobilization in response to SDF-1alpha/CXCL12-induced prolonged activation of extracellular signal-related protein kinase in Jurkat T cells. PMID: 12817019
28. SLP-76 is necessary for T cell receptor stimulation-induced polarization of the T cell's microtubule-organizing center, as it moves toward the interface of the T cell and antigen-presenting cell. PMID: 12847255
29. Study provides the first data to address the mechanisms controlling SLP-76 transcription by providing evidence for several key cis-regulatory elements in the promoter region. PMID: 14662865
30. the proline-rich domain in SLP-76 has a role in subcellular localization and T cell function PMID: 14722089
31. Data suggest that SLP-76 may play a role in signaling pathways by interacting with the p85 subunit of phosphoinositide 3-kinase (PI3K). PMID: 15388330
32. SLP-76 need not interact with SH3(PLC) to activate PLC-gamma1, and the P-I region of SLP-76 serves a structural role that is sequence-independent and is not directly related to protein-protein interactions PMID: 15623534
33. Data show that the adaptor molecules LAT and SLP-76 are specifically targeted by Yersinia to inhibit T cell activation. PMID: 15699071
34. TCR-induced association of Vav3 with SLP-76 is required for its membrane/IS localization and function PMID: 15708849
35. In T cells all SLP-76 proteins are in a approximately 400 kDa complex with the small adaptor protein Grb2-like adaptor protein Gads. PMID: 16356554
36. findings show that retinoic acid(RA) induced the expression of SLP-76, which when co-expressed with an RA-induced receptor, c-FMS, enhanced RA-induced cell differentiation and G0 cell cycle arrest PMID: 16439309
37. The costimulatory effect of CD6 is mediated through phosphorylation-dependent binding of a specific tyrosine residue, 662Y, in its cytoplasmic region to the adaptor SLP-76. PMID: 16914752
38. The P-I region deletion disrupted Vav association and reduced SLP-76-associated kinase activity. PMID: 17148460
39. The integrity of T-cell receptor signaling in vivo is sustained both by strong selection of SLP-76 for the Gads C-SH3 domain and by a capacity to buffer intrinsic crossreactivity. PMID: 17235283
40. phosphorylation of the adaptor molecule SLP-76 is essential for recruitment of the exchange factor Vav leading to Ca(2+) flux and IL-2 production PMID: 17237383
41. Required for activation of IL-2-inducible T cell kinase (ITK); furthermore, an ongoing physical interaction between SLP-76 and ITK is required to maintain ITK in an active conformation. PMID: 17420479
42. SLP-76 relocalizes to integrin-initiated signaling complexes by a mechanism different from that employed during TCR signaling and that SLP-76 relocalization corresponds to SLP-76-dependent integrin function in T cells. PMID: 19667077
43. SLP76 is differentially required for T cell receptor- compared to chemokine C-X-C receptor 4-mediated inside-out signaling pathways regulating T cell adhesion and migration in Jurkat T cells. PMID: 19812192
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亞細胞定位：

Cytoplasm.
組織特異性：

Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
數據庫鏈接：

HGNC: 6529

OMIM: 601603

KEGG: hsa:3937

STRING: 9606.ENSP00000046794

UniGene: Hs.304475

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