ACO1 Recombinant Monoclonal Antibody

說明書

中文名稱：

ACO1重組抗體
貨號：

CSB-RA298469A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: ACO1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 100 kDa
Observed band size: 95-130 kDa

Immunofluorescence staining of HepG2 cell with CSB-RA298469A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 511-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing HepG2 cells stained with CSB-RA298469A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

ACO1 Recombinant Monoclonal Antibody（CSB-RA298469A0HU）是一款針對人源ACO1蛋白的高特異性抗體，適用于多種實驗場景。ACO1（烏頭酸酶1）是一種鐵硫簇結合蛋白，在細胞能量代謝中發揮雙重功能：既參與三羧酸循環催化檸檬酸轉化為異檸檬酸，又在胞質中作為鐵調節蛋白（IRP1）調控鐵代謝相關基因表達，對維持細胞內鐵穩態及氧化應激響應至關重要。該抗體經ELISA、免疫印跡（WB）、免疫熒光（IF）和流式細胞術（FC）嚴格驗證，在推薦稀釋度下（WB 1:500-1:2000；IF 1:50-1:200；FC 1:50-1:200）能特異性識別內源性ACO1蛋白，實驗數據顯示清晰的靶標條帶和精準的亞細胞定位信號。適用于研究能量代謝通路調控機制、鐵硫簇蛋白功能分析、鐵代謝相關疾病模型中的分子機制探索，以及氧化應激條件下細胞適應性反應的分子基礎研究。該試劑為鐵代謝與線粒體功能相關的基礎研究提供可靠工具，特別適用于需要高靈敏度和低交叉反應性的復雜樣本檢測。
Uniprot No.：

P21399
基因名：

ACO1
別名：

Cytoplasmic aconitate hydratase (Aconitase) (EC 4.2.1.3) (Citrate hydro-lyase) (Ferritin repressor protein) (Iron regulatory protein 1) (IRP1) (Iron-responsive element-binding protein 1) (IRE-BP 1), ACO1, IREB1
反應種屬：

Human
免疫原：

A synthesized peptide derived from human ACO1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

22C10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.; Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
基因功能參考文獻：
1. These results support the concept that IRP1 is an oxidative stress biosensor that mediates iron accumulation and cell death when deregulated by mitochondrial dysfunction. PMID: 28502703
2. Results indicate that there is a dysregulation of DMT1 + IRE in IA testes, which might due to the up-regulation of IRP1 and HIF-1A. PMID: 28762519
3. Evaluation of the iron regulatory protein-1 interactome has been presented. PMID: 29330752
4. regulatory circuit involving FBXL5 and CIA acts through both IRPs to control iron metabolism and promote optimal cell growth PMID: 28768766
5. Data suggest that Fe-S cluster of IRP1 plays role in sensing/regulating cellular iron homeostasis; IRP1 alternates between function as cytosolic aconitase [which contains Fe(4)-S(4) cluster in active-site cleft] and function as apoenzyme [which lacks Fe-S cluster] that binds to iron response element stem-loop structures present in several iron regulatory protein transcripts. [REVIEW] PMID: 28615439
6. The SNP rs7874815 in the ACO1 gene was strongly associated with survival in pancreatic cancer. PMID: 26755275
7. Our results confirm the observation that mitoNEET is important in transferring the iron sulfur clusters to the cytosolic aconitase in living cells and the His-87 ligand in mitoNEET plays important role in this process. PMID: 26778000
8. Data show that IRP1 suppressed TfR1/DMT1 (+IRE) expression, limited the cellular iron content and decreased lactate dehydrogenase (LDH) release induced by hypoxia. PMID: 25727755
9. These in vitro studies suggest that human GLRX3 is important for cytosolic Fe-S protein maturation. PMID: 26296460
10. SIRT3 overexpression decreases TfR1 expression by inhibiting IRP1 and represses proliferation in pancreatic cancer cells. PMID: 24909164
11. ACO1 gene expression was positively associated with adipogenic markers in subcutaneous and visceral adipose tissue. PMID: 25550467
12. ACO1 genetic variation is associated with neuropathic pain and pain severity in HIV-infected patients on antiretroviral therapy PMID: 25144566
13. MitoNEET governs a novel trafficking pathway to rebuild an Fe-S cluster into cytosolic aconitase/IRP1. PMID: 25012650
14. Polymer-dependent translocation of IRP-1 in Cd(2+)-exposed cells may underlie effects of Cd(2+) on iron homeostasis. PMID: 25106854
15. Data indicate that JTR-009, a benzimidazole, operated by preventing iron-regulatory protein-1 (IRP1) from binding to the iron-responsive element (IRE) in APP mRNA. PMID: 23935819
16. Aco1 encodes a moonlighting protein that has aconitase activity as well as mRNA binding ability. PMID: 8041788
17. Data indicate that silencing of Grx3 in HeLa cells decreases the activities of several cytosolic Fe/S proteins, including iron-regulatory protein 1, a major component of posttranscriptional iron regulation. PMID: 23615448
18. Studies indicate that several genes have been linked to iron homeostasis, including transferrin (TF), iron regulatory protein 1 (ACO1) and transferrin receptor 2 (TFR2). PMID: 23817740
19. Lead specifically induces dysregulation of IRP1 protein by activating the ERK1/2 signaling pathway indicating a novel role for IRP1 and the ERK/MAPK pathway in vascular endothelial functions. PMID: 22502979
20. The RNA-binding and aconitase forms of IRP-1 can undergo interconversion dependent on the density of cells growing in culture. PMID: 22544036
21. Ggenetic polymorphisms of the iron regulatory protein 1 and 2 genes may be associated with development of age-related macular degeneration. PMID: 22331484
22. Molecular genetic analysis revealed point mutations within the FTL IRE. PMID: 22020773
23. Dual-luciferase reporter assay showed that iron regulatory protein 1 (IRP)1 was directly downregulated by hypoxia-inducible factor 1/hypoxia response element system. PMID: 21360641
24. Single nucleotide polymorphism in ACO1 gene is associated with cutaneous malignant melanoma. PMID: 20574843
25. Selective translational control of the Alzheimer amyloid precursor protein transcript by iron regulatory protein-1. PMID: 20558735
26. ATP/ADP binding maintains IRP-1 in a non-IRE-binding state. PMID: 20569198
27. iron regulatory protein-1-mediated inhibition of hypoxia-inducible factor-2a translation is linked to the anti-inflammatory 15-deoxy-delta12,14-prostaglandin J2 PMID: 20354189
28. gene coding and flanking regions were sequenced and examined for mutations that might modulate the iron burden of individuals harboring the common mutant hemochromatosis HFE genotype or cause hemochromatosis independent of mutations in the HFE gene PMID: 11783942
29. Structural changes associated with switching activities of human iron regulatory protein 1 PMID: 11812787
30. liver levels or this protein are down-regulated in hemochromatosis PMID: 12196178
31. functional in the activation of iron uptake PMID: 12572667
32. data suggest that the differential regulation of IRP1 and IRP2 during hypoxia may be important for cellular adaptation to low oxygen tension. PMID: 12855587
33. A phosphomimetic mutation at Ser-138 renders IRP1 sensitive to iron-dependent degradation. PMID: 12972614
34. Toxoplasma gondii infection resulted in increased activity in the iron response protein IRP1, which, in this state, stabilizes transferrin receptor mRNA from degradation. PMID: 15349772
35. iron-replete HepG2 cells exhibit a narrow range of maximal responsiveness of the IRP-regulatory mechanism PMID: 15543932
36. Apo-IRP1 exists as an equilibrium mixture of monomers and dimers in solution; however, only monomeric IRP1 is observed in complex with the iron regulatory element (IRE). PMID: 15938636
37. Comparison of IRP1 in its aconitase form with known structures of homologous enzymes reveals well-conserved folds and active site environments with significantly different surface shapes and charge distributions. PMID: 16407072
38. IRP1 controls iron homeostasis during cell proliferation, inflammation, and in response to diseases altering cytosolic Fe-S cluster assembly or disassembly. PMID: 16424901
39. IRP1 activity level is higher in monocytes and macrophages of hereditary hemochrosis (HH) patients than in those of control subjects, and is increased in the duodenal samples of the patients with HH and iron-deficiency anemia. PMID: 16503547
40. Overexpression of iron regulatory protein 1 suppresses growth of tumor xenografts PMID: 17127713
41. Results describe thet effect of hypoxia on the binding and subcellular distribution of iron regulatory proteins 1 and 2. PMID: 17200797
42. Thus, proper folding of human iron regulatory protein 1 strongly depends on its cellular environment, in contrast to other members of the aconitase family. PMID: 17244191
43. Although the iron-responsive element does not itself hydrolyze ATP, its presence enhances iron regulatory protein-1's ATPase activity, and ATP hydrolysis results in loss of the complex in gel shift assays. PMID: 17521334
44. analysis of the iron regulatory protein system PMID: 17604281
45. Hypertransfusional (>8 transfusions/year) iron in liver biopsies correlated with increased expression (RNA) for iron regulatory proteins 1 and 2 (3-, 9- to 11-fold) and hepcidin RNA: (5- to 8-fold), while ferritin H and L RNA remained constant. PMID: 17613866
46. Expression of TfR1 mRNA is increased when maternal iron deficiency progressed while there is no change in the expression of IRP1 mRNA in the placenta. PMID: 17877204
47. Downregulated by HBX in human hepatoma cell lines. PMID: 18262302
48. This study shows that expression of the transferrin receptor (TfR) is downregulated, whereas expression of the ferritin heavy chain is upregulated in Chlamydia trachomatis infected HeLa-229 cells. PMID: 19688112
顯示更多

收起更多
亞細胞定位：

Cytoplasm, cytosol.
蛋白家族：

Aconitase/IPM isomerase family
數據庫鏈接：

HGNC: 117

OMIM: 100880

KEGG: hsa:48

STRING: 9606.ENSP00000309477

UniGene: Hs.567229

Most popular with customers

YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human
WDR5 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human