FBP1 Recombinant Monoclonal Antibody

說明書

中文名稱：

FBP1重組抗體
貨號：

CSB-RA787345A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA787345A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA787345A0HU FBP1重組單克隆抗體是針對果糖-1,6-二磷酸酶1（FBP1）靶點設(shè)計的高特異性科研工具，該靶點作為糖異生途徑的關(guān)鍵限速酶，在維持血糖穩(wěn)態(tài)及能量代謝中發(fā)揮重要作用，其異常表達與代謝性疾病及腫瘤發(fā)生密切相關(guān)。本抗體通過重組技術(shù)制備，經(jīng)ELISA和免疫組化（IHC）嚴(yán)格驗證，在IHC應(yīng)用中展現(xiàn)優(yōu)異的組織定位能力，推薦使用稀釋比例為1:50至1:200，可清晰顯示靶蛋白在石蠟包埋組織切片中的分布特征。適用于研究FBP1在肝臟代謝調(diào)控、腫瘤細(xì)胞糖代謝重編程等領(lǐng)域的表達模式，為代謝通路分析、疾病機制探索及藥物靶點研究提供可靠檢測方案，廣泛應(yīng)用于體外樣本的蛋白定性及組織水平的表達定位分析。
Uniprot No.：

P09467
基因名：

FBP1
別名：

Fructose-1,6-bisphosphatase 1 (FBPase 1) (EC 3.1.3.11) (D-fructose-1,6-bisphosphate 1-phosphohydrolase 1) (Liver FBPase), FBP1, FBP
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human FBP1
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4G3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.
基因功能參考文獻：
1. FBP1 modulates the sensitivity of pancreatic cancer cells to BET inhibitors by decreasing the expression of c-Myc. These findings highlight FBP1 could be used as a therapeutic niche for patient-tailored therapies PMID: 30201002
2. Low FBP1 expression is associated with metastasis in cholangiocarcinoma. PMID: 30193944
3. CBX3 serves as a positive regulator of aerobic glycolysis via suppressing of the FBP1 in pancreatic cancer cells. PMID: 29678579
4. Homozygous Alu element insertion in the FBP1 gene is associated with Fructose-1,6-bisphosphatase deficiency. PMID: 28599390
5. decreased levels of FBP1 may be used as a predictor for poor prognosis of cervical cancer patients PMID: 28990097
6. Study identified for the first time that HNF4alpha and C/EBPalpha are important transcriptional regulators for FBP1 expression in human hepatoma HepG2 cells. PMID: 29566023
7. FBP1 mutation was associated with fructose-1,6-bisphosphatase deficiency PMID: 29016355
8. Results identified FBP1 as a promising acute liver failure (ALF) biomarker among energy metabolism-related proteins. It may serve as a short-term prognosis indicator for ALF, with higher serum level of FBP1 correlated with higher ALF-related mortality in human studies. PMID: 28336726
9. Here, the first crystal structure of human liver FBPase in the R-state conformation is presented, determined at a resolution of 2.2 A in a tetragonal setting that exhibits an unusual arrangement of noncrystallographic symmetry (NCS) elements. PMID: 27841754
10. Studied association of fructose 1,6-bisphosphatase 1 (FBP1) expression with fluorine 18 ((18)F) fluorodeoxyglucose (FDG) accumulation in patients with hepatocellular carcinoma. Found that in patients with HCC, both 18F FDG accumulation and tumor grade (from differentiated to undifferentiated) were inversely correlated with the expression of FBP1. PMID: 28387640
11. These findings indicate that FBP1 appears to be a tumor suppressor in hepatocellular carcinoma (HCC). Strategies to restore the levels and activities of FBP1 might be developed to treat patients with HCC. PMID: 27742690
12. FBP1 underexpression is associated with Tumor Progression in Hepatocellular Carcinoma. PMID: 27197151
13. fructose-1,6-bisphosphatase 1 facilitated co-action between Bcl-2 and Beclin 1, which may be important in the mechanism of fructose-1,6-bisphosphatase 1-mediated mitophagy inhibition. In summary, loss of mitophagy by fructose-1,6-bisphosphatase 1-mediated repression promotes apoptosis in breast cancer PMID: 28653874
14. Downregulation of FBP1 promotes gastric cancer metastasis by facilitating EMT and acts as a potential prognostic factor and therapeutic target in gastric cancer. PMID: 27978536
15. we show that EV71 viral proteinase 2A is capable of cleaving far upstream element-binding protein 1 (FBP1), a positive internal ribosome entry sitet rans-acting factor that directly binds to the EV71 5' UTR linker region to promote viral IRES-driven translation PMID: 27780225
16. Cox multivariate regression analysis demonstrated that DUOX1, GLS2, FBP1 and age were independent risk factors for the prognosis of HCC patients after surgery PMID: 27079415
17. Elevated FBP1 is a critical modulator in breast cancer progression by altering glucose metabolism and the activity of Wnt/beta-catenin pathway. PMID: 27780144
18. identified Zinc finger E-box-binding homeobox 1 (ZEB1) bond to FBP1 promoter to enhance DNA methylation in lung cancer cells. Our findings indicate that the down-regulation of FBP1 is a critical oncogenic event in lung cancer progression PMID: 26546081
19. Twelve different mutations were identified in 22 alleles: one missense mutation c.472C > T, one point deletion c.48del, one point duplication c.865dupA, one deletion-insertion, and two splice mutations (c.427-1del and c.825 + 1G > A). PMID: 25601412
20. FBP1 expression, which is closely correlated with c-Myc expression, is an independent prognostic factor and promotes nasopharyngeal carcinoma progression. PMID: 26469968
21. NPM1 promotes aerobic glycolysis and tumor progression in patients with pancreatic cancer by inhibiting FBP1 PMID: 26068981
22. There was a negative correlation with the level of FBP1 and recurrence of the lung cancer PMID: 25844935
23. the gluconeogenic enzyme fructose-1,6-bisphosphatase 1 (FBP1) is uniformly depleted in over six hundred clear cell renal cell carcinoma tumours examined. PMID: 25043030
24. Ca(2+) affects conformation of the catalytic loop 52-72 of muscle FBPase and inhibits its activity by competing with activatory divalent cations, e.g. Mg(2+) and Zn(2+). PMID: 24146906
25. Case Reports: present reliable diagnostic system to verify an FBPase deficiency and find the genetic aberration. PMID: 20151204
26. A novel missense mutation (c.841G>A) in the FBP1 gene seems to be prevalent in Pakistani-Indian patients with fructose-1,6-bisphosphatase deficiency. PMID: 23881342
27. A homozygous c.658delT mutation was detected at exon 5 of the FBP1 gene in the two siblings with FBPase deficiency. PMID: 24007283
28. LSD1 regulates transcription activation of two gluconeogenic genes, FBP1 and G6Pase. PMID: 23755305
29. study indicates that the loss of FBP1 is a critical oncogenic event in epithelial-mesenchymal transition and basal-like breast cancer PMID: 23453623
30. The Identification of FBPase interacting partners with mass spectrometry reveals a set of nuclear proteins involved in cell cycle regulation, mRNA processing and in stabilization of genomic DNA structure. PMID: 22057438
31. This is the first study to identify liver FBPase as a previously unknown regulator of appetite and adiposity and describes a novel process by which the liver participates in body weight regulation. PMID: 22517657
32. AMP binding pattern of the muscle isozyme of fructose-1,6-bisphosphatase is quite similar to that of the liver isozyme and the T conformations of the two isozymes are nearly the same PMID: 22120740
33. epigenetic inactivation of FBP1 is also common in human liver and colon cancer. FBP1 appears to be a functional tumor suppressor involved in the liver and colon carcinogenesis PMID: 22039417
34. Novel compound heterozygous mutations in the fructose-1,6-bisphosphatase gene cause hypoglycemia and lactic acidosis. PMID: 20096900
35. first report on mutations in patients with FBP deficiency of Arab ethnicity,two novel mutations in the FBP1 gene encode for a duplication of two amino acids and a truncation in the FBP1 protein in these families. PMID: 19259699
36. Data show that NF-kappaB functions downstream of Ras to promote epigenetic downregulation of FBP1. PMID: 19881551
37. Data show that EDTA and mercaptoethanol stabilized FBP-1 activity in stored urine. PMID: 19505453
38. liver fructose-1,6-bisphosphatase coupled with phosphofructokinase (PFK) plays a crucial role in the metabolism of pancreatic islet cells PMID: 15965961
39. Upregulation of FBPase in pancreatic islet beta-cells in states of lipid oversupply and type 2 diabetes, contributes to insulin secretory dysfunction. PMID: 18375435
40. Human FBP1 was found to regulate mouse endogenous glucose production and whole body glucose homeostasis in a liver-specific transgenic model. PMID: 18780768
41. this is the first experimental evidence confirming that the KKKGK motif can act as a functional NLS fructose 1,6-bisphosphatase . PMID: 19626708
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相關(guān)疾病：

Fructose-1,6-bisphosphatase deficiency (FBP1D)
蛋白家族：

FBPase class 1 family
組織特異性：

Expressed in pancreatic islets.
數(shù)據(jù)庫鏈接：

HGNC: 3606

OMIM: 229700

KEGG: hsa:2203

STRING: 9606.ENSP00000364475

UniGene: Hs.494496

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