ISL1 Recombinant Monoclonal Antibody

說明書

中文名稱：

ISL1重組抗體
貨號：

CSB-RA941933A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate
All lanes: Islet1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa

IHC image of CSB-RA941933A0HU diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA941933A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

ISL1重組單克隆抗體（CSB-RA941933A0HU）是靶向胰島因子1（ISLIM1）的高特異性科研工具，該轉錄因子在胚胎發育過程中調控胰腺、心臟及神經系統等器官的細胞分化與組織形成，并在成體組織干細胞穩態中發揮重要作用。本產品經多種實驗平臺嚴格驗證，在WesternBlot中可清晰識別天然ISL1蛋白，推薦使用1:500-1:5000稀釋梯度；免疫組化實驗驗證顯示其在1:50-1:200濃度范圍內能精準定位石蠟切片中的靶蛋白表達，同時在ELISA平臺中展現出優異的抗原結合能力。通過重組表達技術和單克隆抗體平臺制備，確保批次間高度一致性，適用于發育生物學研究（如器官發生機制解析）、干細胞命運調控分析以及相關疾病模型的分子機制探索，為研究ISL1在組織再生、先天性發育異常等領域的生物學功能提供可靠檢測方案。
Uniprot No.：

P61371
基因名：

ISL1
別名：

Insulin gene enhancer protein ISL-1 (Islet-1), ISL1
反應種屬：

Human
免疫原：

A synthesized peptide derived from human Islet1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1A1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart.
基因功能參考文獻：
1. ISL1 is a major susceptibility gene for bladder exstrophy and a regulator of urinary tract development. PMID: 28176844
2. Mechanistic analyses integrate this unrecognized anti-atrial function of ISL1 with known and newly identified atrial inducers. In this revised view, ISL1 is antagonized by retinoic acid signaling via a novel player, MEIS2. PMID: 29337667
3. Data show that Islet-1 (ISL1) activated the expression of cyclin B1 (CCNB1), cyclin B2 (CCNB2) and c-myc (c-MYC) genes by binding to the conserved binding sites on their promoters or enhancers. PMID: 27183908
4. results identify Isl1 as a crucial transcription factor that plays essential roles in the gene regulatory program directing development of multiple arcuate neuronal subpopulations. PMID: 27578785
5. ISL-1 is widely expressed in Olfactory neuroblastoma tumors with neuroendocrine differentiation and therefore of limited value in their differential diagnosis PMID: 27543867
6. Data indicate that cells cultured on cardiac muscle laminin (LN)-based substrata in combination with stimulation of the canonical Wnt/beta-catenin pathway showed increased gene expression of ISL1, OCT4, KDR and NKX2.5. PMID: 27052314
7. In bladder exstrophy there is a clear correlation with the a mutation of the chromosome 5 ISL1 (5q11.1) gene. PMID: 26077299
8. SSBP3 Interacts With Islet-1 and Ldb1 to Impact Pancreatic beta-Cell Target Genes PMID: 26495868
9. Concurrent ISL1/HOXA9 methylation in HG-NMIBC reliably predicted tumour recurrence and progression within one year (Positive Predictive Value 91.7%), and was associated with disease-specific mortality PMID: 26332997
10. Isl1 overexpression in embryonic stem cells results in normal electrophysiologically functioning cells. PMID: 25524439
11. The present study identified the first genome-wide significant locus for classic bladder exstrophy at chromosomal region 5q11.1, and provides strong evidence for the hypothesis that ISL1 is the responsible candidate gene in this region. PMID: 25763902
12. Suggest that ISL-1 may be a useful prognostic biomarker and may represent a novel therapeutic target for gastric adenocarcinoma. PMID: 26142548
13. ISL-1 is tightly linked to non-Hodgkin lymphoma proliferation and development by promoting c-Myc transcription, and its aberrant expression was regulated by p-STAT3/p-c-Jun/ISL-1 complex activation. PMID: 25070240
14. Overexpression of ISL1 in human mesnchymal stem cells promotes angiogenesis in vitro and in vivo through increasing secretion of MCP3 and other paracrine factors. PMID: 24578274
15. ISL1 is commonly expressed in rhabdomyosarcoma, particularly the alveolar subtype PMID: 24751901
16. Results suggest that rs1017 ISL1 contributed to the risk of Congenital heart disease (CHD) in Chinese Han people, and the gene may be involved in the formation and development of the heart. PMID: 24634231
17. This study demonstrates that the Isl1-Gata3 transcription regulatory axis is essential for normal pyloric development. PMID: 24674670
18. these results demonstrate a positive feedback regulation of ISL-1 in diffuse large B cell lymphoma but not in pancreatic beta-cells, which might result in the functional diversities of ISL-1 in different physiological and pathological processes. PMID: 24845569
19. We have demonstrated that Islet 1 and PAX8 are not entirely specific for neuroendocrine tumors of pancreatic origin, as they are expressed in a majority of rectal neuroendocrine tumors. PMID: 24037217
20. Overexpression of VEGF-A promotes not only the endothelial specification but also engraftment, proliferation and survival (reduced apoptosis) of the human Isl1+ progenitors in vivo. PMID: 24018375
21. Haplotype TT of ISL1 may increase the risk of congenital heart disease (CHD)in Tianjin Han children. PMID: 24131831
22. findings modify the role of ISL1 as a marker for pancreatic neuroendocrine neoplasms and suggest that ISL1 has a broader involvement in differentiation and growth of neuroendocrine neoplasms than has so far been assumed. PMID: 23503646
23. The methylation status of Homeobox A9 (HOXA9), ISL LIM homeobox 1 (ISL1) and Aldehyde dehydrogenase 1 family, member A3 (ALDH1A3) was significantly associated with decreased gene expression levels PMID: 23436614
24. The findings suggest that ISL1 genetic polymorphisms are associated with occurrence of VSD, thus they may be useful as molecular markers for prediction of VSD. PMID: 23572340
25. ISL1 common variant rs1017 is not associated with increased genetic risk of CHD in the white population. PMID: 23229290
26. This study describes a new gain-of-function p.Asn252Ser variant in the human ISL1 gene, which could potentially lead to greater activation of downstream targets involved in cardiac development, dilation, and hypertrophy. PMID: 23152444
27. Our findings confirm that Isl1 is a sensitive marker of pancreatic origin in cases of metastatic neuroendocrine neoplasms . PMID: 23348208
28. This is the first study which indicates that ISL1 common variant rs1017 may not play a role in sporadic CHD susceptibility in the Chinese population. PMID: 22480195
29. Both Islet 1 and PAX8 are reliable immunohistochemical markers for identifying metastatic neuroendocrine tumors of pancreatic origin. PMID: 22388755
30. The relationship between human ISL1 and FGF10 within the embryonic time window during which the linear heart tube remodels into four chambers, was examined. PMID: 22303449
31. This study establishes ISL1 as a novel transcriptional regulator of LHX3 and describes a potential mechanism for regulation by PITX1. PMID: 22194342
32. Data indicate that, despite the relevant role of ISL1 in thyroid and heart morphogenesis, mutations in its coding region are not associated with TD in our group of patients. PMID: 21060249
33. Two different ISL1 haplotypes contribute to risk of congenital heart disease in white and black/African American populations. PMID: 20520780
34. early first trimester human embryonic heart expresses clusters of Isl1(+)cells, some of which differentiate into cardiomyocytes. PMID: 19896915
35. Seven single nucleotide polymorphisms (SNPs) and one microsatellite in noncoding regions of ISL1 GENE. Four haplotypes formed by six of these SNPs and one microsatellite were associated with type 1 diabetes in Swedish families. PMID: 15161765
36. we demonstrated that ISL1 and BETA2 could activate insulin gene transcription synergistically. PMID: 16321656
37. cultured pancreatic islets contain nestin and Isl-1 positive mesenchymal stem cells with multipotential developmental capacity PMID: 16713999
38. Isl1 could enhance the HNF4alpha-mediated activation of transcription of the HNF1alpha, PPARalpha and insulin I promoters PMID: 17022998
39. Sudy illustrated that GPA2 is positively regulated by isl-1, suggesting that this protein associates with endocrine systems including the pituitary and pancreas. PMID: 17363077
40. beta-catenin signaling regulates proliferation and survival of cardiac progenitors PMID: 17519333
41. An important developmental and regulatory gene ISL1 is uniquely expressed in visceral fat, probably in the preadipocyte. ISL1 may be regulated by adiposity. PMID: 18239644
42. Although the LIM interaction domain of Ldb1 (Ldb1(LID)) and Isl1(LBD) share low levels of sequence homology, X-ray and NMR structures reveal that they bind Lhx3 in an identical manner, that is, Isl1(LBD) mimics Ldb1(LID). PMID: 18583962
43. purified ISL1(+) primordial progenitors are capable of self-renewal and expansion before differentiation into the three major cell types in the heart PMID: 19571884
44. These results suggest that ISL1 is a transcriptional activator for insulin gene expression, and the interactions of ISL1 with BETA2 are required for the transcriptional activity of the insulin gene. PMID: 19619559
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亞細胞定位：

Nucleus.
組織特異性：

Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
數據庫鏈接：

HGNC: 6132

OMIM: 600366

KEGG: hsa:3670

STRING: 9606.ENSP00000230658

UniGene: Hs.505

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