Recombinant Human AP-2 complex subunit alpha-1 (AP2A1), partial

中文名稱：

Recombinant Human AP-2 complex subunit alpha-1(AP2A1) ,partial
貨號(hào)：

CSB-YP001869HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Human AP-2 complex subunit alpha-1(AP2A1) ,partial
貨號(hào)：

CSB-EP001869HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Human AP-2 complex subunit alpha-1(AP2A1) ,partial
貨號(hào)：

CSB-EP001869HU-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Human AP-2 complex subunit alpha-1(AP2A1) ,partial
貨號(hào)：

CSB-BP001869HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Human AP-2 complex subunit alpha-1(AP2A1) ,partial
貨號(hào)：

CSB-MP001869HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

AP2A1
Uniprot No.：

O95782
別名：

100 kDa coated vesicle protein A; Adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; Adaptin alpha A; Adaptin alpha; Adaptor protein complex AP 2 alpha 1 subunit; adaptor protein complex AP 2 subunit alpha 1; Adaptor protein complex AP-2 subunit alpha-1; Adaptor protein complex AP2 alpha 1 subunit; adaptor protein complex AP2 subunit alpha 1; Adaptor related protein complex 2 alpha 1 subunit; Adaptor related protein complex AP 2 alpha 1 subunit; Adaptor related protein complex AP2 alpha 1 subunit; ADTAA; Alpha A adaptin; Alpha adaptin A; Alpha-adaptin A; alpha1 adaptin; Alpha1-adaptin; AP 2 alpha; AP 2 complex subunit alpha 1; AP-2 complex subunit alpha-1; AP2 complex subunit alpha 1; AP2-ALPHA; Ap2a1; AP2A1_HUMAN; CLAPA 1; CLAPA1; Clathrin adaptor complex AP2. alpha subunit; Clathrin assembly protein complex 2 alpha A large chain; Clathrin assembly protein complex 2 alpha-A large chain; Clathrin associated / assembly / adaptor protein large alpha 1; clathrin associated assembly adaptor protein large alpha 1; clathrin associated/assembly/adaptor protein, large, alpha 1; Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunit; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
基因功能參考文獻(xiàn)：
1. Results revealed thirteen novel splice junctions of previously annotated exons. Consecutive nested PCRs led to the determination of the primary structure of seventy-seven novel AP2A1 transcripts, all of which were shown to comprise at least one premature translation termination codon, thus representing nonsense-mediated mRNA decay candidates. PMID: 30081190
2. AP-2alpha depletion enhanced apoptosis, demonstrating that disrupting the HIV-1 Nef:AP-2alpha interaction limits Nef-mediated apoptosis. PMID: 28577469
3. These results lead to a model for the docking of the full AP-2 tetramer to membranes as bound to Nef, such that the cytosolic tail of CD4 is situated to interact with its binding site on Nef. PMID: 24473078
4. A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration. PMID: 22360420
5. AP2alpha negatively regulates HIV-1 replication in the process of nuclear translocation of viral DNA in the cytoplasm or the perinuclear region. PMID: 18178234
6. nef usurps AP-2 complexes to dysregulate Ii (invariant chain (Ii)/major histocompatibility complex class II) trafficking and potentially interfere with antigen presentation in the context of MHC-II PMID: 18524831
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亞細(xì)胞定位：

Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

Adaptor complexes large subunit family
組織特異性：

Expressed in the brain (at protein level). Isoform A: Expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B: Widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
數(shù)據(jù)庫鏈接：

HGNC: 561

OMIM: 601026

KEGG: hsa:160

STRING: 9606.ENSP00000351926

UniGene: Hs.467125