E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Many of the substrates contain a PxIxIT motif and/or a LxVP motif. In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation. In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion. Dephosphorylates heat shock protein HSPB1. Dephosphorylates and activates transcription factor NFATC1. In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32. May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins. Dephosphorylates transcription factor TFEB at 'Ser-211' following Coxsackievirus B3 infection, promoting nuclear translocation.
基因功能參考文獻:
A short-linear interaction motif between residues 337-343 of AKAP79 is the sole PP2B-anchoring determinant sustaining these diverse topologies. PMID: 28967377
Mutation in PPP3CA gene is associated with Severe Neurodevelopmental Disease with Seizures. PMID: 28942967
identify a distinct C-terminal autoinhibitory four-residue sequence in CNAbeta1, (462)LAVP(465), which competitively inhibits substrate dephosphorylation. In vitro and cell-based assays revealed that the CNAbeta1-containing holoenzyme, CNbeta1, is autoinhibited at a single site by either of two inhibitory regions, CBD and LAVP, which block substrate access to the substrate-binding groove. PMID: 28842480
Studies indicate the importance of the AKAP79/PP2B/protein kinase A complex's role in synaptic long-term depression in the CA1 region of the hippocampus. PMID: 27911714
Ca(2+)/calcineurin (CaN)/nuclear factor of activated T-cells (NFAT) c4 axis is required for neuritin-induced Kv4.2 transcriptional expression and potentiation of IA densities in cerebellum granule neurons. PMID: 27307045
CN-A and nephrin were clearly reduced in the kidneys of a 10-year-old boy who had relapsing steroid-resistant nephrotic syndrome. PMID: 27009276
that KIF1Bbeta affects mitochondrial dynamics through calcineurin-dependent dephosphorylation of Dynamin-related protein 1 (DRP1), causing mitochondrial fission and apoptosis PMID: 26812016
for Aspergillus fumigatus, activation of macrophage calcineurin-NFAT occurs via a phagosomal TLR9-dependent and Bruton's tyrosine kinase-dependent signalling pathway that is independent of MyD88 PMID: 25637383
Data indicate that both nuclear factors of activated T cells (NFATs) motifs partially compete for binding but do not fully displace each other on the calcineurin (Cn) epitope. PMID: 24954618
Lysosomal calcium signaling regulates autophagy through calcineurin and TFEB. PMID: 25720963
Human herpesvirus 6B U54 binds the calcineurin (CaN) phosphatase enzyme, causing improper dephosphorylation and nuclear translocation of NFAT (nuclear factor of activated T cells) proteins, resulting in suboptimal IL-2 gene transcription. PMID: 25122797
KSR2 deficiency affects stromal interaction molecule 1 (STIM1)/ORAI1 puncta formation, which is correlated with cytoskeleton disorganization. PMID: 24672054
These findings therefore provide initial support for the novel mechanistic hypothesis that oxidation-induced global and/or local conformational changes within calcineurin PMID: 25286016
PKCepsilon may negatively regulate adverse myocardial remodeling by cooperating with calcineurin to downregulate fibrosis and induce transcription of cardioprotective wound healing genes, including COX-2 PMID: 24298017
Mutations on the hydrophobic face of Calcineurin distal helix disrupt the structure gained upon CaM binding. PMID: 24191726
Bile-induced NF-kappaB activation and acinar cell injury are mediated by calcineurin. PMID: 23744075
Dephosphorylation of Aly1 by calcineurin serves as a regulatory switch to promote Aly1-mediated trafficking to the vacuole. PMID: 23824189
lower expression of PPP3CA and PPP3CB genes in atrium myocardium can be related to expressed postinfarction LV remodeling. PMID: 23888774
PPP3CA down-regulation is associated with hepatocellular carcinoma infected with hepatitis C virus. PMID: 23317196
suggest that CaMKII and calcineurin provide a switch-like mechanism that controls Ca-dependent LIMK1, SSH1L and cofilin activation, and subsequently actin cytoskeletal reorganization PMID: 22270398
Present findings indicate that downregulation of hemoxygenase-1 expression in neutrophils from hypertensive subjects is likely mediated by CN, which acts by hindering translocation to the nucleus of the transcription factor NRF2. PMID: 22739212
Alterations in calcineurin signaling in the caudate nucleus contribute to the pathogenesis of schizophrenia. PMID: 22285318
NHE1 directly binds to calcineurin A and activates downstream NFAT signaling, leading to cardiomyocyte hypertrophy PMID: 22688515
calcineurin crystal of trypsin-resistant catalytic domain belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 161.6, b = 87.4, c = 112.0 A PMID: 22691791
Syndecan-4 is essential for development of concentric myocardial hypertrophy via stretch-induced activation of the calcineurin-NFAT pathway PMID: 22164265
Even though an association of the polymorphisms rs2850328 and rs2395 and breast cancer was not detected in our case-control study population, other variants within the PPP3CA and MARK4 genes may still be associated with breast cancer. PMID: 22506312
A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration. PMID: 22360420
The authors demonstrate that the regulatory domain within calcineurin is unstructured and that it folds upon binding calmodulin, ousting the autoinhibitory domain from the catalytic site. PMID: 22100452
High calcineurin A alpha protein is associated with bone metastasis in small-cell lung cancer. PMID: 21785830
The C allele of protein phosphatase 3 subunit alpha rs3804358 polymorphism was overrepresented in athletes compared with controls, whereas the T allele of protein phosphatase 3 subunit beta rs3763679 polymorphism was underrepresented in athletes. PMID: 21233773
Findings demonstrate that CaN functions as a critical signaling molecule during Th cell activation, regulating Bcl-10 phosphorylation and NF-kappaB activation. PMID: 21674474
calcineurin has a role in the heart and in cardiac disease PMID: 19879877
two new complementary roles for calcineurin in the regulation of the early UPR (Unfolded Protein Responses) PMID: 20700529
data underscore the importance of calcineurin gene in the molecular mechanism of addiction and Alzheimer's diseases PMID: 20590401
CN mediates the Ang II-induced aldosterone synthesis through up-regulation of the CYP11B2 transcription. PMID: 20413672
Data show that in patients with CHF, calpain upregulation was associated with an increase in cleavage of cain/cabin1 and the activation of CaN. PMID: 20514436
Data suggest an association between polymorphisms in PPP3CA, PPP3R1 and PPP3R2 and baseline levels or trainability of endurance phenotype traits. PMID: 20107831
PP2B is an important target of the aberrant acinar cell Ca(2+) rise associated with pathological protease activation and pancreatitis. PMID: 20501444
Observational study and genome-wide association study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379146
CnAalpha, was significantly overexpressed in lung cancer tissues with bone metastasis as compared to tumors with non-bone metastases. PMID: 20422345
The functional differences conferred upon CaN by the alpha or beta catalytic subunit isoforms suggest that the alpha:beta and CaN:substrate ratios may determine the levels of CaN phosphatase activity toward specific substrates. PMID: 12135494
involvement of PKA and PPP2B in the regulation of NF-kappaB in human monocytes PMID: 12586544
there is a 13-amino acid region within CN that is essential for the interaction with NFAT and with two other CN-binding proteins, AKAP79 and Cabin-1 PMID: 15671033
The activation of calcineurin by calpain I in the brain of patients with Alzheimer's disease is reported. PMID: 16150694
These results indicate that calcineurin mediates acetylcholinesterase expression during apoptosis. PMID: 17320203
activation of calcineurin is required for lytic granule exocytosis but it is not the sole Ca2+-dependent step PMID: 17478429
analysis of the secondary structure of calcineurin regulatory region and conformational change induced by calcium/calmodulin binding PMID: 18296442
CHP2 has a role in tumorigenesis and as an activator of the calcineurin/NFAT signaling pathway PMID: 18815128
Study demonstrates that all CaN isoforms display the same cytoplasmic subcellular distribution and are expressed in each tested cell line, differences in substrate specificities may determine specific physiological functions of the distinct isoforms. PMID: 19154138
A conserved docking surface on calcineurin mediates interaction with substrates and immunosuppressants. PMID: 19285944