Recombinant Human Shugoshin 1 (SGO1)

中文名稱：

人SGO1重組蛋白
貨號：

CSB-YP688764HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人SGO1重組蛋白
貨號：

CSB-EP688764HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人SGO1重組蛋白
貨號：

CSB-EP688764HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人SGO1重組蛋白
貨號：

CSB-BP688764HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人SGO1重組蛋白
貨號：

CSB-MP688764HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

SGO1
Uniprot No.：

Q5FBB7
別名：

hSgo 1; hSgo1; NY BR 85; Serologically defined breast cancer antigen NY BR 85 ; Serologically defined breast cancer antigen NY-BR-85; SGO 1; SGO; SGO L1; SGO1; SGOL 1; SGOL1; SGOL1_HUMAN; Shugoshin 1AB protein; Shugoshin 1CD protein; Shugoshin 1EFprotein; Shugoshin 1GH protein; Shugoshin 1KL protein; Shugoshin like 1 (S. pombe); Shugoshin like 1; Shugoshin-like 1
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區域：

1-561
氨基酸序列

MAKERCLKKS FQDSLEDIKK RMKEKRNKNL AEIGKRRSFI AAPCQIITNT STLLKNYQDN NKMLVLALEN EKSKVKEAQD IILQLRKECY YLTCQLYALK GKLTSQQTVE PAQNQEICSS GMDPNSDDSS RNLFVKDLPQ IPLEETELPG QGESFQIEDQ IPTIPQDTLG VDFDSGEAKS TDNVLPRTVS VRSSLKKHCN SICQFDSLDD FETSHLAGKS FEFERVGFLD PLVNMHIPEN VQHNACQWSK DQVNLSPKLI QPGTFTKTKE DILESKSEQT KSKQRDTQER KREEKRKANR RKSKRMSKYK ENKSENKKTV PQKKMHKSVS SNDAYNFNLE EGVHLTPFRQ KVSNDSNREE NNESEVSLCE SSGSGDDSDD LYLPTCKYIQ NPTSNSDRPV TRPLAKRALK YTDEKETEGS KPTKTPTTTP PETQQSPHLS LKDITNVSLY PVVKIRRLSL SPKKNKASPA VALPKRRCTA SVNYKEPTLA SKLRRGDPFT DLCFLNSPIF KQKKDLRRSK KRALEVSPAK EAIFILYYVR EFVSRFPDCR KCKLETHICL R
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase.
基因功能參考文獻：
1. Aurora B kinase interacts with and phosphorylates Sgo1. Aurora B-mediated phosphorylation of Sgo1 regulates the distribution of Sgo1 between centromeres and chromosome arms. PMID: 25451264
2. SGOL1 variant B induces abnormal mitosis and resistance to taxane in non-small cell lung cancers. PMID: 24146025
3. Cohesin complex is shown to be a target of the prophase pathway at centrosomes and protected by Sgo1-dependent PP2A recruitment. PMID: 26365192
4. Sgo1 co-recruits Aurora B and PP2A to centromeres of unattached chromosomes. PMID: 25892238
5. Data indicate essential role of shugoshin-like protein 1 (Sgo1) in the maintenance of a proper mitotic progression in hepatoma cells and suggest that Sgo1 is a promising oncotarget for hepatocellular carcinoma (HCC). PMID: 25638162
6. Results show that Sgo1 is first recruited to kinetochores by H2A-pT120, and the kinetochore-bound Sgo1 is released by centromeric transcription. PMID: 26190260
7. Mutations in SGOL1 cause a novel cohesinopathy affecting heart and gut rhythm. PMID: 25282101
8. Bub1-mediated H2A phosphorylation penetrates kinetochores and that this histone mark contributes to a tension-sensitive Sgo1-based molecular switch for chromosome segregation. PMID: 24055156
9. The most significant association with MaxDrinks was observed with SNP rs11128951 (p = 4.27 x 10(-8)) near SGOL1 gene at 3p24.3. PMID: 23953852
10. Frameshift mutations of SGOL1 and PDS5B and the loss of their expression may be a feature of gastric and colorectal cancers with high microsatellite instability. PMID: 23850494
11. SGO1 promotes multidrug resistance of gastric cancer cells and may be useful as a novel therapeutic target for preventing or reversing multidrug resistance PMID: 23564482
12. Lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth PMID: 22161216
13. These results suggest that SGOL1-P1 may function as a negative factor to native SGOL1, and that abundant expression of SGOL1-P1 may be responsible for chromosomal instability. PMID: 21532624
14. HP1alpha binding by INCENP or Shugoshin 1 (Sgo1) is dispensable for centromeric cohesion protection during mitosis of human cells, but might regulate yet unknown interphase functions of the chromosome passenger complex (CPC) or Sgo1 at the centromeres. PMID: 21346195
15. Data show that human germinal center-associated nuclear protein (GANP) is critically involved in cell proliferation at the mitotic phase through its selective support of shugoshin-1 mRNA export. PMID: 20384790
16. Bub1 protects centromeric cohesion through Shugoshin in mitosis PMID: 15604152
17. A shugoshin-like protein associates with centromeres during prophase and disappears at the onset of anaphase PMID: 15737064
18. a specific subtype of serine/threonine protein phosphatase 2A (PP2A) associates with human shugoshin PMID: 16541025
19. Bub1 targets PP2A to centromeres, which in turn maintains Sgo1 at centromeres by counteracting Plk1-mediated chromosome removal of Sgo1. PMID: 16580887
20. depletion of Sgo1 results in precocious chromosomal segregation and massive mitotic arrest PMID: 16628005
21. Mutation in the SGOL1 D-box causes transient metaphase arrest, and leads to defects in chromosome alignment and segregation. PMID: 17448445
22. Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1. PMID: 17617734
23. Results suggest that NEK2A-mediated phosphorylation of Sgo1 (SGOL1) provides a link between centromeric cohesion and spindle microtubule attachment at the kinetochores. PMID: 17621308
24. Centriole splitting induced by Sgo1 depletion or expression of a dominant negative mutant is suppressed by ectopic expression of sSgo1 or by Plk1 knockdown. PMID: 18331714
25. findings suggest that hSgo1-downregulated colorectal cancers have a clinicopathological character of chromosome instability (CIN), and hSgo1 downregulation leads to CIN in colorectal cancer cells. PMID: 18635744
26. the direct link between HP1 and shugoshin is conserved in human cells PMID: 18716626
27. the spindle checkpoint kinase Bub1 contributes to the maintenance of Sgo1 steady-state protein levels in an APC/C-independent mechanism. PMID: 19015261
28. Report structure and function of the PP2A-shugoshin interaction. PMID: 19716788
29. A comprehensive review of Sgo1 function in eukaryote at meiosis and mitosis. PMID: 16112668
顯示更多

收起更多
相關疾病：

Chronic atrial and intestinal dysrhythmia (CAID)
亞細胞定位：

Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
蛋白家族：

Shugoshin family
組織特異性：

Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested.
數據庫鏈接：

HGNC: 25088

OMIM: 609168

KEGG: hsa:151648

STRING: 9606.ENSP00000263753

UniGene: Hs.105153