Recombinant Mouse Calcitonin gene-related peptide 1 (Calca), partial

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貨號：

CSB-EP859124MO
說明書：

MSDS Datasheet
規格：
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Calca
Uniprot No.：

Q99JA0
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

16.8 kDa
表達區域：

83-119aa
氨基酸序列

SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSEAF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
基因功能參考文獻：
1. Hepatic PCT expression is upregulated in ALF. Hepatic macrophages but not hepatocytes are the cell source of hepatic PCT expression. PMID: 29913443
2. alphaCGRP overexpression causes an increase in thermal reaction and downregulation of the cardiovascular system, presumably due in increased levels of alphaCGRP. PMID: 30068853
3. Glucagon-like peptide (GLP)-1 modulates epithelial ion transport indirectly by activating calcitonin gene-related peptide (CGRP)-containing submucosal enteric neurons in the mouse colon. This GLP-1-CGRP response was area-specific and could potentially contribute to the diarrheal side effect of certain GLP-1 receptor therapeutics. PMID: 28695626
4. This study demonstrated that the expression patterns of the calcitonin-related polypeptide alpha and tyrosine hydroxylase formed opposing gradients, with tyrosine hydroxylase being preferentially expressed in apical and calcitonin-related polypeptide alpha in basal type II afferent neurons, indicating heterogeneity among type II afferent neurons. PMID: 29055051
5. CGRP-1 is essential for pain-associated plasticity in the central amygdala. PMID: 28833700
6. These results suggest that neuronal TRPV1 signaling in periodontal tissue is crucial for the regulation of osteoclastogenesis via the neuropeptide CGRP. PMID: 27388773
7. alphaCGRP played a synergic role in angiogenesis and osseointegration, partly as a consequence of its vasodilative activity. PMID: 29421662
8. results indicate that CGRP regulates macrophage polarization and inhibits inflammation in murine macrophages. PMID: 28892747
9. The results indicate a central role of neuroendocrine peptide aminoprocalcitonin (NPCT) in Alzheimer disease pathogenesis and suggest NPCT as a potential biomarker and therapeutic target. PMID: 27497681
10. In addition to its actions in the cardiovascular system, endogenous CGRP is a key regulator of metabolism and energy homeostasis in vivo. PMID: 28324021
11. findings establish CGRP neurons in the parabrachial nucleus as key mediators of cancer-induced appetite suppression and associated behavioral changes. PMID: 28581479
12. These results identify an important, possibly dual role of CGRP in Amyotrophic lateral sclerosis pathogenesis. PMID: 27554772
13. functional inactivation of CGRP neurons markedly increases meal size, with meal frequency being reduced in a compensatory manner, and renders mice insensitive to the anorexic effects of meal-related satiety peptides. PMID: 27166945
14. Mouse and human heart valves expressed mRNAs for the CRL ligands adrenomedullin (AM), adrenomedullin-2 (AM-2) and calcitonin gene-related peptide (CGRP) and for their receptor components, i.e., CRL and receptor-activity-modifying proteins 1-3. PMID: 27553639
15. The abundance of mRNA of CGRP and bone matrix markers (Col I, Col II, and OPN) was low at P5 in the tibia. These differences in CGRP and other mRNAs may induce a different manner of ossification between the mandible and tibia. Therefore, a time lag of ossification occurs between the mandible and tibia during foetal development. PMID: 28348418
16. Suggest that cochlear function is enhanced by maturation of CGRP receptor complexes. PMID: 27440744
17. the generation of Tregs may be promoted by naive CD4+ T cell: neuron interaction through the release of neuropeptide CGRP. PMID: 27022966
18. The observations of this study imply that Adelta afferents expressing CGRP-IR transduce information from different sites within the skin. PMID: 26010480
19. SHH may affect alveolar bone healing by interacting with CGRP-positive sensory neurons and thus regulate the socket's healing process after tooth extraction. PMID: 26427874
20. TRPM8 signaling in mucosal sensory neurons is indispensable for the regulation of innate inflammatory responses via the neuropeptide CGRP. PMID: 25269705
21. CGRP suppresses the development of leaky choroidal neovascularization through negative regulation of inflammation. PMID: 25857228
22. CT has a role in skeletal biology and S1P acts as an osteoanabolic molecule and has a role in crosstalk between osteoclasts and osteoblasts PMID: 25333900
23. CGRP is an inhibitory transmitter that shapes peripheral taste signals via serotonergic signaling during processing gustatory information in taste buds. PMID: 26377461
24. The increased responsiveness to intraplantar calcitonin gene-related peptide suggests that the peripheral actions of calcitonin gene-related peptide are enhanced as a result of the neurofibromin deficit. PMID: 25184332
25. This study provides direct ultrastructural evidences for the synaptic contacts between CGRP-positive terminals and GABAergic neurons within the central nucleus of amygdala. PMID: 24794145
26. activation of periosteal mineralizing surfaces in response to mechanical loading of bone is CGRPalpha-dependent in vivo. PMID: 25536054
27. Sensory neuron-derived CGRP sustains mechanical hypersensitivity and spinal microglial reactivity in collagen-induced arthritis, suggesting that central mechanisms play critical roles in chronic inflammatory pain. PMID: 25707377
28. The parabrachial CGRP neurons mediate a gastrointestinal distress signal required to establish conditioned taste aversion. PMID: 25788675
29. findings uncover a context-dependent molecular mechanism of TRPV1 algesic sensitization and a previously unrecognized role of alphaCGRP in LDCV mobilization in peptidergic nociceptors PMID: 25489075
30. The results of this study indicated that the enhanced behavioral responses to cold stimuli in CGRPalpha sensory neuron-ablated mice are dependent on functional TRPM8. PMID: 25406633
31. demonstrated that simultaneous downregulation of PPARgamma and upregulation of CGRP was efficient in suppressing adipogenic differentiation of BMSCs and promoting their osteogenic differentiation PMID: 24633961
32. communication through CGRP as a novel costimulatory pathway promoting the development of a regulatory phenotype of TLR4-stimulated macrophages. PMID: 25316186
33. neuropeptides CGRP and VIP have an important role in suppressing bone resorptive activities through RANKL/OPG pathway, similar to mechanical loading. PMID: 24717410
34. These results suggest that contribution of the alphaCGRP system depends on the modality of pain and the stage of inflammation PMID: 24992521
35. This study showed that the neurotransmitter CGRP plays a key role in ensuring VOR efficacy. PMID: 25080603
36. CGRP regulates Th1 type reactions. PMID: 24466057
37. There is an ongoing role of alpha-calcitonin gene-related peptide as part of a protective network against hypertension, vascular hypertrophy, and oxidative stress. PMID: 24516108
38. The activation of alphaCGRP may induce other neuropeptides associated with immunomodulation at central nervous system. level PMID: 24021706
39. Alpha-CGRP plays a significant protective role in heart failure which may be mediated by decreased inflammation, cell death, and fibrosis. PMID: 23816470
40. SP-, CGRP-, and VGLUT2-mediated transmission together were found to play a role in the development of mechanical hyperalgesia after persistent inflammation. PMID: 24275230
41. Alpha-CGRP may play an important role in the regulation of kainic acid-induced pyramidal cell death in CA3 region of the hippocampus. PMID: 23587546
42. Data indicate that transient receptor potential ion channels and neuropeptides substance P and calcitonin gene-related peptide have important roles in formaldehyde promoted-asthma. PMID: 23671638
43. The results clearly demonstrate that endogenous CGRP suppresses the oxidative stress and VSMC proliferation induced by vascular injury. PMID: 23416515
44. DNA methylation changes were noted in the Calca gene during chronic cystitis in a murine model. PMID: 23806407
45. Deletion of the gene encoding calcitonin and calcitonin gene-related peptide alpha did not influence the course of sepsis in three separate mouse models of infection. PMID: 23526213
46. CT/alpha-CGRP modulates bone cell activity in PIO in aged mice in a way that is distinct from young animals PMID: 23357366
47. CGRP-immunoreactive nerve fibers were specifically increased with the development of food allergy. PMID: 23261435
48. The CGRP/cyclic AMP/protein kinase A (PKA) pathway is important in regulating the differentiation of T helper (Th)9 cell subset. PMID: 23509367
49. The data of this study revealed that CGRPalpha sensory neurons encode heat and itch and tonically cross-inhibit cold-responsive spinal neurons. PMID: 23523592
50. Hydrogen sulfide-evoked release of CGRP from sensory nerves induces increases in microcirculation of mouse ear following activation of TRPA1 receptors. PMID: 22721614
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亞細胞定位：

Secreted.
蛋白家族：

Calcitonin family
組織特異性：

Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult.
數據庫鏈接：

KEGG: mmu:12310

UniGene: Mm.4361