Recombinant Mouse E3 ubiquitin-protein ligase CBL-B (Cblb), partial

中文名稱：

Recombinant Mouse E3 ubiquitin-protein ligase CBL-B(Cblb) ,partial
貨號：

CSB-YP667545MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mouse E3 ubiquitin-protein ligase CBL-B(Cblb) ,partial
貨號：

CSB-EP667545MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Mouse E3 ubiquitin-protein ligase CBL-B(Cblb) ,partial
貨號：

CSB-EP667545MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mouse E3 ubiquitin-protein ligase CBL-B(Cblb) ,partial
貨號：

CSB-BP667545MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mouse E3 ubiquitin-protein ligase CBL-B(Cblb) ,partial
貨號：

CSB-MP667545MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Cblb
Uniprot No.：

Q3TTA7
別名：

CblbE3 ubiquitin-protein ligase CBL-B; EC 2.3.2.27; Casitas B-lineage lymphoma proto-oncogene b; RING-type E3 ubiquitin transferase CBL-B; SH3-binding protein CBL-B; Signal transduction protein CBL-B
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA.
基因功能參考文獻：
1. The establishment of a new model of concurrent tissue-selective CBL/CBL-B deletion should allow a clear assessment of the tumor-intrinsic roles of CBL/CBL-B in non-myeloid malignancies and help test the potential for CBL/CBL-B inactivation in immunotherapy of tumors. PMID: 27276677
2. GSK3 catalyzes two previously unreported phosphorylation events at Ser(476) and Ser(480) of Cbl-b. Constitutive activation of PKB in vivo results in a loss of tolerance that is mediated through the downregulation of Cbl-b. The PI3K-PKB-GSK-3 pathway is a novel regulatory axis that is important for controlling the decision between T cell activation and tolerance via Cbl-b. PMID: 29109121
3. These studies reveal a novel, cell-autonomous requirement of CBL and CBL-B in epithelial stem cell maintenance during organ development and remodeling through modulation of mTOR signaling. PMID: 28100467
4. CBLB directs polyubiquitination of dectin-1 and dectin-2, two key pattern-recognition receptors for sensing Candida albicans, and their downstream kinase SYK, thus inhibiting dectin-1- and dectin-2-mediated innate immune responses. PMID: 27428899
5. CBLB controls proximal C-type lectin receptor signaling in macrophages and dendritic cells. CBLB associates with SYK and ubiquitinates SYK, dectin-1, and dectin-2 after fungal recognition. CBLB deficiency results in increased inflammasome activation, enhanced reactive oxygen species production, and increased fungal killing. PMID: 27428901
6. study indicates that Cbl-b negatively regulates CLR-mediated antifungal innate immunity PMID: 27432944
7. Fasudil, a clinically safe ROCK inhibitor, decreases disease burden in a Cbl/Cbl-b deficiency-driven murine model of myeloproliferative disorders. PMID: 26177294
8. Mechanistically, NFATc1 induces Nur77 expression at late stage of osteoclast differentiation; in turn, Nur77 transcriptionally up-regulates E3 ubiquitin ligase Cbl-b, which triggers NFATc1 protein degradation. PMID: 26173181
9. Silencing Cbl-b significantly enhanced T lymphocyte function and T lymphocyte cytotoxicity activity PMID: 25249301
10. mechanisms have therapeutic implications for reducing beta-cell proliferation in insulinomas by inhibiting phospho-HLXB9 or its interaction with Nono and modulating the expression of its direct (Cblb) or indirect (c-Met) targets PMID: 26342078
11. SHP-1 regulates Cbl-b-mediated T cell responses by controlling its tyrosine phosphorylation and ubiquitination PMID: 26416283
12. Cbl-b(-/-) T cells demonstrate significant lymph node trafficking abnormalities. PMID: 25829233
13. These results indicated that NF-kappaB downregulated Cbl-b by binding and suppressing Cbl-b promoter in T cell activation PMID: 25762784
14. Cbl-b, together with Stub1, ubiquitinate Foxp3, and regulate tTreg development. PMID: 25560411
15. Whereas Cbl-PI3K interaction regulates differentiation and survival, bone resorption is predominantly regulated by Cbl-b in osteoclasts. PMID: 24470255
16. Cbl-b is the E3 ubiquitin ligase for Stat6. Cbl-b regulates Th9 in both Stat6-dependent and -independent mechanisms. PMID: 24508458
17. The results demonstrate an essential and non-redundant role for Cbl-b in controlling TGFbetaR signaling by directly targeting SMAD7 for degradation during T cell responses in vitro and in vivo. PMID: 23709694
18. Cbl-b, by promoting the ubiquitination and degradation of DDR2, functions as a negative regulator in the DDR2 signaling pathway. PMID: 24631539
19. genetic deletion of the E3 ubiquitin ligase Cbl-b (casitas B-lineage lymphoma-b) or targeted inactivation of its E3 ligase activity licenses natural killer (NK) cells to spontaneously reject metastatic tumours PMID: 24553136
20. Cbl-b plays only a limited role in the induction of antigen-specific T cell responses by murine bone marrow-derived dendritic cells in vitro and in vivo. PMID: 23762309
21. study demonstrates that Cbl-b regulates the fate of inducible regulatory T cells via controlling the threshold for T cell activation PMID: 23749633
22. Cbl-b inhibits T cell activation by suppressing Pten inactivation independently of its ubiquitin ligase activity. PMID: 22763434
23. Data suggest that targeting Cbl-b might serve as a novel 'adjuvant approach', suitable to augment the effectiveness of established anti-cancer immunotherapies. PMID: 21383769
24. c-Cbl as well as Cbl-b may play important roles in Hsp90 inhibitor-induced degradation of Flt3-ITD through the ubiquitin proteasome system PMID: 21768087
25. Cbl-b is a critical factor in maintaining lung homeostasis upon environmental exposure to aeroallergens. PMID: 20738317
26. Interplay between Cblb-dependent T cell anergy and other mechanisms prevents organ-specific islet-cell autoimmunity in transgenic mice. PMID: 21248249
27. Selective genetic inactivation of Cbl-b E3 ligase activity phenocopies the T cell responses observed when total Cbl-b is ablated, resulting in T cell hyperactivation, spontaneous autoimmunity, and impaired induction of T cell anergy in vivo. PMID: 21248250
28. The resilts of this study suggested that Cbl-b is an important regulatory factor for cytotoxic T-cell infiltration via RANTES production in macrophages. PMID: 21254087
29. show that, in contrast to Cbl or Cbl-b single-deficient mice, concurrent loss of Cbl and Cbl-b in the HSC compartment leads to an early-onset lethal myeloproliferative disease in mice PMID: 20805496
30. Data show that intrinsic regulation of peripheral T cells by CBL-B serves a uniquely critical role as a failsafe against clinical onset of autoimmune disease in AIRE deficiency. PMID: 20668237
31. Data suggest that the regulatory abnormalities in Cbl-b(-/-) mice are related to defects in Teff, not Treg, function. PMID: 20624942
32. gene deletion of both Cbl-b and Itch leads to augmented T cell activation and spontaneous autoimmunity PMID: 20637659
33. Results indicate that Cbl-b controls Runx2 expression at the post-translational level. PMID: 20578243
34. This study reveals that Foxo factors promote transcription of the Foxp3 gene in induced T reg cells, and thus provides new mechanistic insight into Foxo-mediated T cell regulation. PMID: 20439537
35. Cbl-b plays a positive modulatory role in GPVI-dependent platelet signaling, which translates to an important regulatory role in hemostasis and thrombosis in vivo PMID: 20400514
36. Cbl-b and itch are key regulators of peripheral T-cell tolerance [review] PMID: 20395198
37. NFATc1 interacts endogenously with c-Src, c-Cbl, and Cbl-b in osteoclasts. PMID: 20037154
38. novel E3 ubiquitin-protein ligase; role in regulation of immune response - review PMID: 11826757
39. Cbl-b negatively regulates BCR signaling by targeting Syk for ubiquitination. PMID: 12771181
40. both c-Cbl and Cbl-b can initiate a phosphatidylinositol 3-kinase/protein kinase B-independent signaling pathway critical to insulin-stimulated GLUT4 translocation PMID: 12842890
41. Cbl-b and c-Cbl have divergent effects on Fc epsilon RI signal transduction and that Cbl-b, but not c-Cbl, functions as a negative regulator of Fc epsilon RI-induced degranulation. PMID: 15265912
42. activated KIT in turn induces phosphorylation and activation of Cbl proteins PMID: 15315962
43. Study provides a molecular framework for Cbl-b/Vav1-dependent and Cbl-b/Vav1-independent CD28 activation pathways essential for the organization of secondary lymphoid tissues and in vivo immunity to viral infection. PMID: 15661906
44. E3 ubiquitin ligase Cbl-b plays an integral role in T cell TGF-beta signaling; its absence results in multifunctional TGF-beta-related defects that have important disease-related implications. PMID: 16424156
45. cbl-b is a negative regulator of long-term memory, and its neuronal mechanism regulates synaptic transmission in the hippocampus PMID: 16549761
46. results suggest that Cbl-b induces resistance of osteoblasts to IGF-I during denervation by increasing IRS-1 degradation and that Cbl-b-mediated modification of IGF-I signaling may contribute to decreased bone formation during denervation PMID: 16734387
47. Cbl proteins play a critical role in establishing the MHC-dependent CD4(+) and CD8(+) T cell development programs. PMID: 17045823
48. Cbl-b functions as a potent negative regulator of cytokines that promote allergic and inflammatory reactions. PMID: 17056522
49. Ablation of Cbl-b can be an efficient strategy for eliciting immune responses against both inoculated and spontaneous tumors. PMID: 17364027
50. identify Cbl-b as a key signaling molecule that controls spontaneous antitumor activity of cytotoxic T cells in different cancer models PMID: 17403934
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亞細胞定位：

Cytoplasm. Note=In adipocytes, translocates to the plasma membrane upon insulin stimulation.
數(shù)據(jù)庫鏈接：

KEGG: mmu:208650

STRING: 10090.ENSMUSP00000110115

UniGene: Mm.328206