Recombinant Mouse GDNF family receptor alpha-like (Gfral), partial (Active)

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中文名稱：

小鼠Gfral重組蛋白
貨號：

CSB-MP750964MO1
說明書：

MSDS Datasheet
規格：

￥828
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Gfral at 5 μg/ml can bind Mouse Gdf15 (CSB-MP859530MO), the EC₅₀ is 7.926-10.52 ng/mL. Biological Activity Assay
其他：

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靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Mouse Gfral at 5 μg/mL can bind Mouse Gdf15 (CSB-MP859530MO), the EC₅₀ is 7.926-10.52 ng/mL.
基因名：

Gfral
Uniprot No.：

Q6SJE0
分子結構：
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

42.1 kDa
表達區域：

20-349aa
氨基酸序列

QTNDCAHLIQKCLIDANGCEQSWRSMEDTCLTPGDSCKINNSLHCNLSIQALVEKNFQFKECLCMDDLHCTVNKLFGKKCTNKTDNMEKDNKDKWNLTTTPFYHGFKQMQSCLEVTEACVGDVVCNAQLALYLKACSANGNLCDVKHCQAAIRFFYQNMPFNTAQMLAFCDCAQSDIPCQQSKETLHSKPCALNIVPPPTCLSVIHTCRNDELCRTHYRTFQTECWPHITGKCHEDETCISMLGKQDLTCSGSESCRAAFLGTFGTVLQVPCACRGVTQAEEHVCMIFQHMLHSKSCFNYPTPNVKDISSYEKKNSKEITLTGFNSFFNG
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The DNA fragment encoding the region (Gln20-Gly349) of the mouse GFRAL was expressed with an N-terminal 10xHis-tag and a C-terminal Myc-tag in mammalian cells. The resultant product is the recombinant mouse GFRAL protein. This GFRAL protein has been validated to be active by binding to the mouse GDF15 in a functional ELISA, with the EC₅₀ of 7.926-10.52 ng/mL. Its purity reaches up to 95% measured by SDS-PAGE. It migrated to the molecular weight band of about 45 kDa on the gel. The glycosylation makes the apparent molecular mass slightly higher than the theoretical one. Its endotoxin content is less than 1.0 EU/ug determined by the LAL method. This GFRAL protein is in stock now.

GFRAL is the exclusive receptor for GDF15 which regulates food intake, energy expenditure, and body weight in response to metabolic and toxin-induced stresses. Studies have found that GFRAL is expressed in the posterior brain region of the mouse central nervous system. The GDF15/GFRAL/RET signaling pathway provides new strategies and therapeutic targets for the treatment of metabolism-related diseases, such as obesity, type II diabetes, and pathological overnutrition and malnutrition.
Datasheet & COA：

Please contact us to get it.

產品評價

平均分:

5.0分 - 1 個評價

實驗應用：藥物相關研究

產品評價：我使用CSB-MP750964MO1產品進行功能ELISA實驗，EC50為4.701-10.78 ng/ml，蛋白有完整的生物活性。買的凍干粉，按照說明書很好溶解。蛋白性質穩定，純度高，后續實驗進行的很順利！還會繼續采購，推薦推薦~~

By 翟老師

靶點詳情

功能：

Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
基因功能參考文獻：
1. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
亞細胞定位：

Cell membrane; Single-pass membrane protein; Extracellular side.
蛋白家族：

GDNFR family
組織特異性：

Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
數據庫鏈接：

KEGG: mmu:404194

UniGene: Mm.213862