Recombinant Mouse Glial fibrillary acidic protein (Gfap)

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文獻引用 (1)

中文名稱：

小鼠Gfap重組蛋白
貨號：

CSB-EP009369MO
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP009369MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) GFAP.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP009369MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) GFAP.
其他：

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Gfap
Uniprot No.：

P03995
別名：

GfapGlial fibrillary acidic protein; GFAP
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

65.9kDa
表達區(qū)域：

1-430aa
氨基酸序列

MERRRITSARRSYASETVVRGLGPSRQLGTMPRFSLSRMTPPLPARVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNFAQDLGTLRQKLQDETNLRLEAENNLAAYRQEADEATLARVDLERKVESLEEEIQFLRKIYEEEVRELREQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVATSNMQETEEWYRSKFADLTDAASRNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHARESASYQEALARLEEEGQSLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKDSKQEHKDVVM
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

Gfap是一種細胞骨架蛋白，它與微管和微絲一起構成大多數(shù)真核細胞的細胞骨架，為其他細胞或細胞外基質接觸的質膜提供機械支持，具有維護星形膠質細胞形態(tài)穩(wěn)定、參與血腦屏障形成、調節(jié)突觸功能等多種生物學功能，還參與細胞遷移、運動和有絲分裂。這種蛋白質在中樞神經系統(tǒng)的星形膠質細胞中特異性表達，因此被認為是星形膠質細胞的特異性標志物。

在實驗室研究中，Gfap經常被用作研究工具，因為它的表達模式和功能使其成為研究星形膠質細胞及其功能的理想選擇。華美生物提供大腸桿菌表達的全長重組小鼠膠質纖維酸性蛋白（Gfap），由重組DNA技術制備而成，可作為研究各種生理和病理狀態(tài)下細胞行為的重要工具。
Datasheet & COA：

Please contact us to get it.

引用文獻

Glial fibrillary acidic protein (GFAP) is a novel biomarker for the prediction of autoimmune diabetes Pang Z.et al,FASEB J,2017

產品評價

靶點詳情

功能：

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
基因功能參考文獻：
1. This report the successful prediction and validation of Gfap as an miR-3099 target gene using a combination of bioinformatics resources with enrichment of annotations based on functional ontologies and a spatio-temporal expression dataset. PMID: 28597341
2. These results indicate that autoantibodies against GFAP could serve as a predictive marker for the development of overt autoimmune diabetes. PMID: 28546444
3. GFAP is specifically expressed in the auricular chondrocytes, and assumes a pivotal role in resistance against mechanical stress. PMID: 28063220
4. compared open-skull and thinned-skull imaging methods for two-photon laser microscopy of live astrocytes in neocortex of GFAP-GFP transgenic mice PMID: 28107381
5. work reveals that an Alexander disease-causing mutation alters GFAP turnover kinetics in vivo and provides an essential foundation for future studies aimed at preventing or reducing the accumulation of GFAP. PMID: 28223355
6. Tat expression or GFAP expression led to formation of GFAP aggregates and induction of unfolded protein response (UPR) and endoplasmic reticulum (ER) stress in astrocytes. PMID: 27609520
7. Study provides evidence that transcription of one of the astrocyte-specific genes, Gfap, is cooperatively regulated by co-expressed genes and their regulatory factors. PMID: 27041678
8. This study demonstrated the GFAP-ApoE4 mice exhibited motor impairments when compared to GFAP-ApoE3 and wild-type mice. PMID: 26892275
9. PINK1 deficiency causes defects in GFAP-positive astrogliogenesis during brain development. PMID: 26746235
10. Gnasxl deficiency does not directly affect glial development in the hypothalamus, since it is expressed in neurons, and Gfap-positive astrocytes and tanycytes appear normal during early postnatal stages. PMID: 27080240
11. Induction of glial cytokine expression was sequential, aligned with active sickness behavior, and preceded increased Iba-1 or GFAP immunoreactivity after lipopolysaccharide challenge PMID: 26470014
12. Study provides a mechanistic link between the GFAP mutations/overexpression and the symptoms in those affected with Type II Alexander disease PMID: 26190408
13. Study described GFAP-expressing non-myelinating Schwann cells in the lung, validated a transgenic mouse line that drives expression of cre under a GFAP promoter PMID: 26442852
14. findings thus show that the inability to produce GFAP and Vim affects normal retinal physiology and that the effect of IF deficiency on retinal cell survival differs, depending on the underlying pathologic condition PMID: 26251181
15. CUL4B as a negative regulator of GFAP expression during neural development. PMID: 26025376
16. Astrocytes deficient of GFAP and vimentin showed decreased Notch signal sending competence and altered expression of Notch signaling pathway-related genes PMID: 26118771
17. Absence of GFAP, or both GFAP and vimentin, alters Alzheimer's disease-induced changes in gene expression profile of astrocytes, showing a compensation of the decrease of neuronal support genes and a trend for a higher inflammatory expression profile PMID: 25731615
18. Data indicate that glial fibrillary acidic protein (GFAP) was up-regulated in satellite glial cells (SGCs) in dorsal root ganglia 14 days after streptozotocin injection. PMID: 25312986
19. Findings demonstrate that ENT1 regulates GFAP expression and possibly astrocyte function PMID: 25365803
20. Data suggest that prenatal alterations in expression of various fetal brain proteins (including down-regulation of Gfap) are associated with aberrant behavioral characteristics of transgenic mice that model autism-like behavior. PMID: 25849768
21. Study shows that increased levels of astrocytic GFAP can contribute to increases on inter-ictal spikes but do not represent a risk factor for appearance of post-traumatic seizures, even when there is increase in reactive gliosis PMID: 25069089
22. The Phactr4 signals were not associated with F-actin fibers but were closely associated with intermediate filaments such as nestin and glial fibrillary acidic protein (GFAP) fibers. PMID: 24748504
23. Report increasing glial fibrillary acidic protein expression and electron microscopic features of brain edema in rodent cerebral malaria. PMID: 24966914
24. absence of GFAP and vimentin in glial cells does not seem to affect the outcome after peripheral motoneuron injury but may have an important effect on the response dynamics PMID: 24223940
25. the astrocyte became activated, exhibiting significantly increased levels of GFAP expression directly related to the level of HIV/VSV replication PMID: 24254728
26. traumatic scratch injury to astrocytes triggered a calcium influx from the extracellular compartment and activated the JNK/c-Jun/AP-1 pathway to switch on GFAP PMID: 24123203
27. Data show that CD8 T cells reactive to glial fibrillary acidic protein (GFAP), a protein expressed in astrocytes, drive unique aspects of inflammatory central nervous system autoimmunity. PMID: 24591371
28. Brain levels of GFAP and Tau proteins decreased significantly at 6 h and increased considerably at 24 h after repeated blast exposures. Plasma samples showed a similar initial decrease and later increase over this timeframe. PMID: 23933206
29. Data indicate that Gfapdelta is expressed in the in developing mouse brain sub-ventricular zones in accordance with the described localization in the developing and adult human brain. PMID: 23991052
30. GFAP was found to be downregulated in HSV-1 acute infection in cornea and upregulated in late stage, suggesting that GFAP might play some role during HSV-1 infection in cornea. PMID: 23758602
31. Data show that neurofibromatosis type 1 (NF1)-inactivation results in a cell-autonomous increase in glial fibrillary acidic protein+ (GFAP+), but not in NG2 proteoglycan NG2+, cell proliferation in vitro. PMID: 23318450
32. this study demonistrated that mouse models of Alexander disease exhibit significant pathology in GFAP-positive radial glia-like cells in the dentate gyrus, and suffer from deficits in adult neurogenesis. PMID: 24259590
33. These studies demonstrate that transactivation of the Gfap promoter is an early and sustained indicator of the disease process in the mouse. PMID: 23432455
34. These data suggest that all astroglia cells in the developing and adolescent mouse brain express GFAPdelta, regardless of their neurogenic capabilities. PMID: 23285135
35. The GFAP-stained intensity of the retinal area is increased in contralateral eyes and decreased in retinal ganglion cells of eyes with laser-induced ocular hypertension. PMID: 22583833
36. Postulate that glial cells with increased Gfap expression support the elongation of new neurites from retinal ganglion cells possibly by providing a scaffold for outgrowth. PMID: 23259929
37. GFAP expression is almost not affected by melatonin treatment in aged mice. PMID: 22200709
38. differential regulation of GFAP isoforms is not involved in the reorganization of the intermediate filament network in reactive gliosis or in neurogenesis in the mouse brain. PMID: 22912745
39. CD44-positive cells are APCs in the early postnatal cerebellum; surviving cells gradually express glial fibrillary acidic protein GFAP), a marker for mature astrocytes, indicating differentiation into mature astrocytes is the default for these cells. PMID: 21732075
40. GFAP-negative astrocytes are fully inflammation-competent, displaying phenotypic heterogeneity as is commonly observed in brain astrocytes. PMID: 22072312
41. In a mouse model of amyotrophic lateral sclerosis (ALS), GFAP is not necessary for the initiation of disease; instead, it plays some modulatory roles in the progression of ALS. PMID: 21453731
42. Treadmill exercise training decreased the expression of GFAP in the striatum of chronic Parkinsonian mice, which can partially explain the beneficial neuroprotective role of exercise in patients with parkinson disease. PMID: 21725169
43. The study shows opposing pattern of nestin and glial fibrillary acidic protein expression in mouse hippocampus occuring in early postnatal development, suggesting that it is important for neural differentiation and positioning in the hippocampus. PMID: 21368556
44. In the mouse there is a slight increase in the number of GFAP positive cells in the white matter after 3 days of severe cerebral contusion trauma. PMID: 20479526
45. Increased expression of GFAP in Muller cells of mer knockout mice occur at P20d in the peripheral retina and P4w in the central retina. GFAP expression in Muller cells appears to be a secondary response to the loss of retinal neurons. PMID: 20497693
46. One protein, GFAP (glial fibrillary acidic protein), was found to be elevated in the LINCL mice compared with normal controls in both isolated storage bodies and a lysosome-enriched subcellular fraction that contains storage material. PMID: 20370715
47. The results of this study suggested that GFAP is necessary for morphological retention and distribution of reactive astrocytes during prion disease, and that there is a GFAP-dependent function of glial filaments in reactive astrocytes. PMID: 19931516
48. Report demonstrated for the first time that GFAPdelta is specifically expressed in radial glia and SVZ neural progenitors during human brain development. PMID: 20040497
49. The exact expression of glial fibrillary acidic protein (GFAP) in trigeminal ganglion and dental pulp. PMID: 11838710
50. Human influenza viral infection in utero alters GFAP immunoreactivity in the developing brains of neonatal mice. PMID: 12140787
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亞細胞定位：

Cytoplasm.
蛋白家族：

Intermediate filament family
組織特異性：

Brain; isoform 2 expressed at 20-fold lower level than isoform 1.
數(shù)據(jù)庫鏈接：

KEGG: mmu:14580

STRING: 10090.ENSMUSP00000064691

UniGene: Mm.1239