Recombinant Mouse Glutamate dehydrogenase 1, mitochondrial (Glud1)
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中文名稱：小鼠Glud1重組蛋白
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貨號：CSB-EP009544MO-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：
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Uniprot No.：
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別名：Glud1; Glud; Glutamate dehydrogenase 1; mitochondrial; GDH 1; EC 1.4.1.3
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種屬：Mus musculus (Mouse)
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蛋白長度：Full Length of Mature Protein
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表達區域：54-558
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氨基酸序列SEAAADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLK TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG DKTFVVQGFG NVGLHSMRYL HRFGAKCVGV GESDGSIWNP DGIDPKELED FKLQHGSILG FPKAKVYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPVVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY NEAGVTFT
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
靶點詳情
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功能：Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
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基因功能參考文獻：
- mRNA and protein levels of GluD1 were increased in fetal brain of foxg1(+/-) mice. mRNA and protein levels of GluD1 were decreased in adult brain of foxg1(+/-) mice. PMID: 26443267
- lack of glutamate oxidation in brain-specific GDH null CnsGlud1-/- mice resulted in a central energy-deprivation state with increased ADP/ATP ratios and phospho-AMPK in the hypothalamus. PMID: 26440896
- This study demonstrated a critical requirement for GluD1 in normal spine development in the cortex and hippocampus. PMID: 25721396
- The GDH activity in mice is highest in the liver with NAD(+) as a coenzyme and highest GDH activity was determined at a glutamate concentration of 10 mM. PMID: 25124006
- Age-related increases of glutamate were observed only in the striatum of the glutamate dehydrogenase 1 mice PMID: 24442550
- Glutamate hyperactivity caused gene expression changes in the hippocampus at all ages PMID: 24593767
- GluD1 regulates the connectivity of parallel fiber-interneuron synapses and promotes differentiation of interneurons. PMID: 24872547
- The GDH1 is a key metabolic enzyme with emerging roles in insulin regulation. MitoNEET forms a covalent complex with GDH1 through disulfide bond formation and acts as an activator. PMID: 24295216
- GluD1 is crucial for normal functioning of synapses and absence of GluD1 leads to specific abnormalities in learning and memory. PMID: 23560106
- Permissive levels of glutamate are required for the full development of glucose-stimulated insulin secretion and glutamate dehydrogenase plays an indispensable role in this process. PMID: 22875990
- Mice deficient in Glud1 exhibit deficient oxidative catabolism of glutamate in astrocytes, showing that GDH is required for Krebs cycle pathway; however, lack of GDH modifies metabolic handling of glutamate without altering synaptic transmission. PMID: 22924626
- These results suggest that GluD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex. PMID: 22138648
- Data show that Balb/c Glud1 transgenic mice had significantly increased depolarization-induced glutamate release compared with wild type littermates. PMID: 21446915
- In betaGlud1(-/-) mice, the reduced secretory capacity resulted in lower plasma insulin levels in response to both feeding and glucose load, while body weight gain was preserved PMID: 19015267
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亞細胞定位：Mitochondrion. Endoplasmic reticulum.
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蛋白家族：Glu/Leu/Phe/Val dehydrogenases family
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