Recombinant Mouse Poly [ADP-ribose] polymerase 2 (Parp2)
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中文名稱：小鼠Parp2重組蛋白
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貨號：CSB-EP017466MO-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：
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Uniprot No.：
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別名：Parp2; Adprt2; Adprtl2; Aspartl2; Poly [ADP-ribose] polymerase 2; PARP-2; mPARP-2; EC 2.4.2.30; ADP-ribosyltransferase diphtheria toxin-like 2; ARTD2; DNA ADP-ribosyltransferase PARP2; EC 2.4.2.-; NAD(+) ADP-ribosyltransferase 2; ADPRT-2; Poly[ADP-ribose] synthase 2; pADPRT-2; Protein poly-ADP-ribosyltransferase PARP2; EC 2.4.2.-
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種屬：Mus musculus (Mouse)
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蛋白長度：Full length protein
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表達區域：1-559
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氨基酸序列MAPRRQRSGS GRRVLNEAKK VDNGNKATED DSPPGKKMRT CQRKGPMAGG KDADRTKDNR DSVKTLLLKG KAPVDPECAA KLGKAHVYCE GDDVYDVMLN QTNLQFNNNK YYLIQLLEDD AQRNFSVWMR WGRVGKTGQH SLVTCSGDLN KAKEIFQKKF LDKTKNNWED RENFEKVPGK YDMLQMDYAA STQDESKTKE EETLKPESQL DLRVQELLKL ICNVQTMEEM MIEMKYDTKR APLGKLTVAQ IKAGYQSLKK IEDCIRAGQH GRALVEACNE FYTRIPHDFG LSIPPVIRTE KELSDKVKLL EALGDIEIAL KLVKSERQGL EHPLDQHYRN LHCALRPLDH ESNEFKVISQ YLQSTHAPTH KDYTMTLLDV FEVEKEGEKE AFREDLPNRM LLWHGSRLSN WVGILSHGLR VAPPEAPITG YMFGKGIYFA DMSSKSANYC FASRLKNTGL LLLSEVALGQ CNELLEANPK AQGLLRGKHS TKGMGKMAPS PAHFITLNGS TVPLGPASDT GILNPEGYTL NYNEFIVYSP NQVRMRYLLK IQFNFLQLW
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
靶點詳情
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功能：Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site. PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex.
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基因功能參考文獻：
- PARP2 is preferentially activated by poly(ADP-ribose) (PAR) and subsequently catalyzes branched PAR chain synthesis. PMID: 30104678
- Peripheral T-cell number was not affected in single PARP-2-deficient mice. Double-deficiency in PARP-1/PARP-2 in T-cells led to highly aggressive T-cell lymphomas. PMID: 28181505
- PARP2 protein deficiency protected mice from Concanavalin A -induced Liver Damage. PMID: 28751426
- Activation of either PARP-1 or -2 is likely to play a role in muscle protein catabolism via oxidative stress, NF-kappaB signaling, and enhanced proteasomal degradation in cancer-induced cachexia. PMID: 28177129
- PARP1/2 inhibitor simmiparib causes growth inhibition in cancer cell- or tissue-derived xenografts in nude mice. PMID: 27847302
- The findings highlight specific non-overlapping functions of PARP1 and PARP2 at H2AX-deficient chromatin during replicative phases of the cell cycle and uncover a unique requirement for PARP1 in nonhomologous end-joining-deficient cells. PMID: 27373144
- Data show reduced tumor burden through increased oxidative stress in lung adenocarcinoma cells of PARP-1 and PARP-2 knockout mice. PMID: 26700152
- PARP-2 has an essential role in erythropoiesis by limiting replicative stress in erythroid progenitors. PMID: 25501596
- PARP-1 and -2 play a role in cancer-induced cachexia, thus selective pharmacological inhibition of PARP-1 and -2 may be of interest in clinical settings PMID: 26432600
- the depletion of PARP-2 leads to lower HDL levels which represent a risk factor to cardiovascular diseases. PMID: 24365238
- This study represents the first description of a significant role for PARP-2 in neuroinflammation and neurological dysfunction in Experimental autoimmune encephalomyelitis PMID: 23607899
- our data show that PARP-2 can directly regulate base excision repair proteins PMID: 23357680
- Our results show that Parp-2 plays essential roles in the surveillance of genome integrity of stem cell hematopoiesis by orchestrating DNA repair and restraining p53-induced and Puma-mediated apoptosis. PMID: 23678004
- PARP2 activity mobilizes intracellular TRPM2 channels in a Parp1-/- background. PMID: 22751932
- Identify PARP-2 as a mediator of doxorubicin toxicity by regulating vascular SIRT1 activity and mitochondrial biogenesis. PMID: 21921080
- Inhibition of PARP1 and PARP2 (by genetic or pharmacological means) leads to increased TOP2B activity in elongating spermatids. PMID: 21228215
- PARP-2 deletion in mice increases SIRT1 levels, promotes energy expenditure, and increases mitochondrial content. PMID: 21459329
- role of PARP-2 activity in the maintenance of telomere integrity; PARP-2 physically binds to TRF2 with high affinity PMID: 14749375
- crystal structure of the catalytic fragment of murine PARP-2, at 2.8 A resolution PMID: 14739238
- We conclude that PARP-2 contributes substantially to nuclear translocation of AIF and infarct size after transient focal cerebral ischemia in male mice, but that protection is disproportionate to the attenuation of overall PARP activity. PMID: 20236222
- a synergistic interaction between Parp-2 and p53 in tumour suppression through the role of Parp-2 in DNA-damage response and genome integrity surveillance PMID: 20154718
- Caspase-8 not only triggers and amplifies the apoptotic process at cytoplasmic sites but can also act as an executioner at nuclear levels where it cleaves PARP-2 PMID: 12065591
- functional interaction with poly ADP ribose pomymerase 1 and role of that interaction in chromosome stability and embryo development PMID: 12727891
- genetic interactions between PARP-1, PARP-2, Atm play an essential role during early embryogenesis [review] PMID: 15279798
- role for PARP-2 as an important mediator of T-cell survival during thymopoiesis by preventing the activation of DNA damage-dependent apoptotic response during the multiple rounds of TCRalpha rearrangements preceding a positively selected TCR PMID: 16946705
- These findings indicate that, in addition to its well known role in DNA repair, Parp-2 exerts essential functions during meiosis I and haploid gamete differentiation. PMID: 17001008
- PPAR-2 controls adipocyte differentiation and adipose tissue function through the regulation of the activity of the retinoid X receptor/PPARgamma heterodimer PMID: 17951580
- These results provide evidence that acetylation of PARP-2 is a key post-translational modification that may regulate DNA binding and consequently also the enzymatic activity of PARP-2. PMID: 18436469
- Selective PARP-2 inhibitors increase apoptosis in hippocampal slices but protect cortical cells in models of post-ischaemic brain damage. PMID: 19422384
- Data show that Parp2 interacts significantly with both the transition protein TP2 and the transition chaperone HSPA2, whereas Parp1 binds weakly to HSPA2. PMID: 19607827
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亞細胞定位：Nucleus. Chromosome.
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組織特異性：Widely expressed; the highest levels were in testis followed by ovary. Expression is correlated with proliferation, with higher levels occurring during early fetal development and organogenesis and in the highly proliferative cell compartments of adult.
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