Recombinant Mouse Tyrosine-protein kinase JAK2 (Jak2), partial
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中文名稱：小鼠Jak2重組蛋白
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貨號：CSB-EP726782MO-B
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說明書：
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規(guī)格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產(chǎn)品詳情
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純度：>85% (SDS-PAGE)
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基因名：
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Uniprot No.：
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別名：Jak2; Tyrosine-protein kinase JAK2; EC 2.7.10.2; Janus kinase 2; JAK-2
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種屬：Mus musculus (Mouse)
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蛋白長度：Partial
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產(chǎn)品評價
靶點詳情
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功能：Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
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基因功能參考文獻：
- SOCS2 negatively regulates the development of natural killer cells by inhibiting JAK2 activity via direct interaction. PMID: 28383049
- HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. PMID: 29650953
- Janus kinase 2 protein (JAK2) and beta-catenin were found to interact with cadherin-22 (Cdh22) and involved in CDH22 signaling in female germ line stem cells (FGSCs). PMID: 29063123
- While expression of mutant Jak2 was necessary for leukemia induction, neither its continued expression nor enzymatic activity was required to maintain leukemia survival and rapid proliferation PMID: 29907650
- High JAK2 expression is associated with graft-versus-host disease. PMID: 29382747
- Results demonstrate that JAK2 deficiency delays tumor formation in GH-transgenic mice, which is linked to insignificant oncogenic STAT3 signaling and reduced reactive oxygen species-induced oxidative damage. PMID: 27713471
- Reg3g acts as an immunosuppressive promoter by activating the JAK2/STAT3 signaling pathway in dendritic cells and triggering the generation of an immunosuppressive tumor microenvironment. PMID: 28880262
- High JAK2 expression is associated with skeletal muscle wasting in cancer cachexia. PMID: 28489606
- Data (including data from studies in knockout mice) suggest that various tissues utilize Jak2 signaling to regulate insulin, glucose, and lipid metabolism; adipocyte Jak2 appears to regulate hepatic insulin resistance in mechanism independent of adiposity (obesity), liver lipid content (non-alcoholic fatty liver disease), and hepatic insulin signaling. PMID: 29203511
- Advanced glycation end products induce apoptosis and inflammation in mouse podocytes through CXCL9-mediated JAK2/STAT3 pathway activation. PMID: 28849106
- Cilostazol protects MIR injury by activating a PPARgamma/JAK2/STAT3 pathway. PMID: 28810537
- These results suggest that JAK2 regulated the microfilaments aggregation during the mouse oocyte maturation. PMID: 28615348
- alpha7nAChR stimulation reduced neuroinflammation via activation of the JAK2-STAT3 pathway, thereby ameliorating the short- and long-term sequelae after intracerebral hemorrhage . PMID: 28529954
- After Dox treatment, the protein expression of p-Jak2 and p-Stat3 levels was significantly increased in MT(+/+) cardiomyocytes, suggesting that the JAK2/STAT3 pathway was partially involved in MT protection against Dox-induced cardiotoxicity PMID: 26526549
- CIS interacted with phosphorylated EpoR at Y401, which was critical for the activation of STAT5 and ERK. PMID: 28038963
- Cell volume homeostasis requiring Na+/H+ exchange signaled by JAK2 first becomes prominent during mouse embryonic development at the late one-cell stage. PMID: 28339658
- a novel signaling axis that regulates JAK2 in normal and malignant HSPCs and suggest new therapeutic strategies for treating CBL(mut) myeloid malignancies. PMID: 28611190
- In summary, JAK2 functions in a homeostatic capacity in podocytes by facilitating autophagy. It does this by regulating the expression of the transcription factor TFEB that is necessary for normal autophagic-lysosomal function PMID: 28424277
- these findings demonstrate that expression of Hmga2 cooperates with Jak2(V617F) in the pathogenesis of Mmyelofibrosis. PMID: 28637665
- Study shows that Jak2 with V617F mutation induces murine model for myeloproliferative neoplasm. PMID: 28668884
- results suggest that loss of Ezh2 cooperates with Jak2V617F in the development of myelofibrosis in Jak2V617F PMID: 27081096
- JAK2-V617F-expressing mice treated with an Ezh2 inhibitor showed higher platelet counts than vehicle controls. Our data support the proposed tumor suppressor function of EZH2 PMID: 27401344
- JAK2 has a role in hepatocarcinogenesis through regulating critical inflammatory pathways PMID: 28100771
- nascent hematopoietic stem cells are resistant to the JAK2V617F mutation and show no decrease in repopulation or self-renewal and no increase in DNA damage, even in the presence of 2 mutant copies. PMID: 26864339
- The results showed that AG490 inhibited (p)-JAK2 and RANKL expression. PMID: 28278513
- these results have revealed that phosphorylation of Tyr-343, Tyr-460, and Tyr-464 in EpoR underlies JAK2 V617F mutant-induced tumorigenesis. PMID: 27998978
- this study shows that ampelopsin reduces inflammatory response of microglial cells through inhibition of JAK2-STAT3 signaling PMID: 27998743
- At the molecular level, administration of liraglutide induced the expression of PDX-1, MafA, p-JAK2 and p-Stat3 in high-fat diet-induced diabetes model to relatively normal levels. PMID: 28290604
- this study shows that resokaempferol inhibits the inflammatory response in activated macrophages by blocking the activation of the JAK2 pathway PMID: 27261558
- Data show that protein tyrosine phosphatase 1B (PTP1B)-deficiency enhanced JAK2-STAT3 signaling in hypothalamic microglia and attenuated inflammation. PMID: 28094236
- Fos-zippered GHR tails and Jak2, both purified from baculovirus-infected insect cells, interacted via box1 with a binding affinity of approximately 40nM. PMID: 26859362
- Single agent BMS-911543 Jak2 inhibitor has distinct inhibitory effects on STAT5 signaling in genetically engineered mice with pancreatic ductal carcinomas. PMID: 26575024
- JAK2 plays a critical role in brown adipose tissue function and adaptive thermogenesis PMID: 26515423
- The critical role of Jak2 in mediating prolactin-induced luminal maturation of mammary epithelial cells. PMID: 26318719
- It increases inflammatory markers in microglia, so that targeting it may be a potential strategy for reducing neuroinflammation in Alzheimer's disease. PMID: 26227742
- Findings indicate that CD24(+) antigen cells play a role in tumor migration and metastasis and support Janus kinase 2 protein (JAK2) as a therapeutic target in ovarian cancer. PMID: 25969154
- JAK2(V617I) resulted in cytokine hyperresponsiveness without constitutive activation in the absence of ligand, whereas JAK2(V617F) resulted in constitutive activation PMID: 26458983
- The pro-inflammatory cytokine, interferon-gamma may contribute to diarrhea associated with intestinal inflammation in part through regulation of the epithelial aquaporin-1 water channel via a non-classical JAK/STAT receptor signalling pathway. PMID: 25793528
- JAK2 participates in the signalling, regulating the voltage-gated K(+) channel KCNA3. PMID: 25644777
- PRDX6 promotes lung tumor development via its mediated and CCL5-associated activation of the JAK2/STAT3 pathway. PMID: 25582888
- The data support an important role of LIF-JAK2-STAT3 regulation in the development of colonic adenocarcinoma-related cachexia. PMID: 26092726
- Studied the liver-specific role of PTP1B in GH signaling; found under 48-hour fasting GH-resistant conditions, GH stimulation in vivo led to a robust stimulation of the JAK-STAT signalling pathway. PMID: 24948418
- HDAC2 might be a downstream effector of Jak2 to mediate cardiac hypertrophic response by pressure overload or Ang-II. PMID: 25380525
- Deletion of Stat3 in hematopoietic cells from JAK2-V617F mice did not ameliorate the course of Myeloproliferative neoplasms, but rather enhanced thrombocytosis and shortened the overall survival. PMID: 25595737
- these findings demonstrate that inhibiting the JAK2/STAT3 signaling pathway is protective against noise-induced cochlear tissue damage and loss of hearing sensitivity. PMID: 25275304
- This work provides insight into the functional consequences of JAK2V617F-TET2 comutation in myeloproliferative neoplasms PMID: 25281607
- Mpl expression, but not Tpo, is fundamental in the development of JAK2V617F(+) myeloproliferative neoplasms PMID: 25339357
- Our studies demonstrate that MPN can be initiated from a single HSC and illustrate that JAK2-V617F has complex effects on HSC biology. PMID: 25288396
- Jak2 plays a critical role in the maintenance and function of adult hematopoietic stem cells. PMID: 24677703
- We demonstrated that JAK2V617F-harboring cells cause paracrine DNA damage accumulation through secretion of lipocalin-2. PMID: 25217696
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亞細胞定位：Endomembrane system; Peripheral membrane protein. Cytoplasm. Nucleus.
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蛋白家族：Protein kinase superfamily, Tyr protein kinase family, JAK subfamily
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組織特異性：Ubiquitously expressed throughout most tissues.
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