Recombinant Mouse Vitronectin (Vtn)

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中文名稱：

小鼠Vtn重組蛋白
品名簡稱：

Recombinant Mouse Vtn protein
貨號：

CSB-EP025944MO
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Vtn
Uniprot No.：

P29788
別名：

(VN)(S-protein)(Serum-spreading factor)
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

60.2 kDa
表達區(qū)域：

20-478aa
氨基酸序列

DQESCKGRCTQGFMASKKCQCDELCTYYQSCCADYMEQCKPQVTRGDVFTMPEDDYWSYDYVEEPKNNTNTGVQPENTSPPGDLNPRTDGTLKPTAFLDPEEQPSTPAPKVEQQEEILRPDTTDQGTPEFPEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDETAVRPGYPKLIQDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPGYPRNISEGFSGIPDNVDAAFALPAHRYSGRERVYFFKGKQYWEYEFQQQPSQEECEGSSLSAVFEHFALLQRDSWENIFELLFWGRSSDGAREPQFISRNWHGVPGKVDAAMAGRIYVTGSLSHSAQAKKQKSKRRSRKRYRSRRGRGHRRSQSSNSRRSSRSIWFSLFSSEESGLGTYNNYDYDMDWLVPATCEPIQSVYFFSGDKYYRVNLRTRRVDSVNPPYPRSIAQYWLGCPTSEK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
基因功能參考文獻：
1. The effects of genetic alterations in PAI-1 expression, pharmacologic PAI-1 inhibition and recombinant PAI-1 on smooth muscle cell vitronectin (VN) expression were studied, and vascular VN expression in wild-type and PAI-1-deficient mice was assessed. PMID: 29028290
2. this study demonstrates in a mouse infection model that Vn reduces complement-mediated killing of Yersinia enterocolitica O:9 E40 and, thus, improves bacterial survival PMID: 27798934
3. the TGF-beta-PAI-1-vitronectin-integrin axis may represent a critical convergence of factors in determining the alveolar epithelial cell apoptotic response during lung injury. PMID: 27106291
4. In vtn knockout mice, neuronal differentiation in the cerebellum was suppressed compared with wild-type mice. PMID: 26640242
5. PAI-1, through its interaction with Vn, exerts multiple deleterious mechanisms to induce AKI. PMID: 25799354
6. These data suggest that adhesion to vitronectin allows for the uncoupling of fibronectin fibril formation from downstream signaling events by reducing alpha5beta1 integrin activation and fibronectin fibril extension. PMID: 24509439
7. Report role of vitronectin in the regulation of angiogenesis induced by VEGF. PMID: 24603119
8. Vitronectin inhibits efferocytosis through interactions with apoptotic cells as well as with macrophages. PMID: 23345331
9. VN plays a previously unrecognized role in regulating endothelial permeability via conformational- and integrin-dependent effects on VE-cadherin trafficking. PMID: 22606350
10. ability of vitronectin to enhance neutrophil viability was dependent on activation of phosphatidylinositol 3-kinase and extracellular signal-regulated kinase 1/2 kinases, but not on the p38 kinase PMID: 22281987
11. These results demonstrate that vitronectin exerts a deleterious role independently from complement, by directing PAI-dependent fibrinolysis in the glomerular compartment. PMID: 21764994
12. the vitronectin-binding capacity of PAI-1 was the primary determinant required for its ability to exacerbate lung scarring induced by intratracheal bleomycin administration PMID: 21734232
13. Suggest that despite significant interstitial Vtn deposition in the ureteral obstruction model of chronic kidney disease, its fibrogenic role is either nonessential or redundant. PMID: 21270094
14. Plasminogen activator inhibitor-1 and vitronectin expression level and stoichiometry regulate vascular smooth muscle cell migration through physiological collagen matrices PMID: 20492459
15. Endogenous vitronectin promotes neointima formation in carotid arteries. PMID: 12067901
16. regulation of cell spreading on vitronectin by ERK1 association with alphaVbeta3 integrin PMID: 12393886
17. In a carotid artery ligation model, vitronectin (VN) knockout mice generate a larger intima than wild-type as a result of more extensive smooth muscle cell (SMC)proliferation, demonstrating that VN inhibits thrombin-mediated SMC proliferation. PMID: 12482822
18. vitronectin can selectively regulate inactivating potassium current in embryonic hippocampal neurons PMID: 12562917
19. competition with plasminogen activator inhibitor type-1 for alphavbeta3 binding inhibits insulin signaling PMID: 12603314
20. in contrast to released platelet granule Vn which enhances platelet aggregation, plasma Vn inhibits platelet aggregation PMID: 15733060
21. There is an increase of vitronectin and integrin alphav in the retina and the optic nerve after optic nerve injury. Induction of Vitronectin and integrin alphav may have an important role in the regeneration of adult mammalian retinal ganglion cells. PMID: 16479252
22. PrP(C) plays an important role in axonal growth, and this function may be rescued in PrP(C)-knockout animals by integrin compensatory mechanisms. PMID: 17504807
23. a novel vitronectin-dependent mechanism contributing to the development of acute lung injury. PMID: 17982099
24. VN is a key determinant of the antiproliferative effect of PAI-1 overexpression. PMID: 19574558
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亞細胞定位：

Secreted, extracellular space.
組織特異性：

Plasma.
數(shù)據(jù)庫鏈接：

KEGG: mmu:22370

STRING: 10090.ENSMUSP00000017488

UniGene: Mm.3667