Recombinant Rat Glial fibrillary acidic protein (Gfap)

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中文名稱：

大鼠Gfap重組蛋白
貨號：

CSB-EP009369RAa0
說明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Gfap
Uniprot No.：

P47819
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length
來源：

E.coli
分子量：

55.9 kDa
表達區(qū)域：

1-430aa
氨基酸序列

MERRRITSARRSYASSETMVRGHGPTRHLGTIPRLSLSRMTPPLPARVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTTNSARLEVERDNLTQDLGTLRQKLQDETNLRLEAENNLAVYRQEADEATLARVDLERKVESLEEEIQFLRKIHEEEVRELQEQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVATSNMQETEEWYRSKFADLTDVASRNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHARESASYQEALARLEEEGQSLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
基因功能參考文獻：
1. demonstrate increased DNA methylation coupled with decreased histone acetylation at the Gfap promoter leading to suppression of Gfap expression under maternal hypothyroidism PMID: 29852171
2. The present study found that social isolation during adolescence resulted in abnormal locomotor, emotional and cognitive behaviors and increased the expression of GFAP, ANXA2 and VIM in PFC of adult rats PMID: 28705472
3. GFAP-positive structures were present and exhibited a tendency to become linear on both sides, with an increased density on the left. NFAP-positive expression was present in the left treated limb with a disorganized pattern PMID: 28652433
4. We found that GFAP exhibited enhanced stability upon the addition of two equivalents of each ligands with ceftriaxone imparting a more spontaneous interactions and a more ordered complex system than phenytoin PMID: 27133445
5. reduction of GFAP+ cell density was in agreement with a lower expression of GFAP protein PMID: 27579183
6. Astrocyte expression of GLAST/GFAP was reduced via JAK1/JAK2/STAT3 signaling pathway after exposure to sevoflurane. PMID: 27003918
7. Upon ependymal stem cells differentiation, Cx50 expression favors glial cell fate, since higher expression levels, endogenous or by over-expression of Cx50, augmented the expression of the astrocyte marker GFAP and impaired the neuronal marker Tuj1. PMID: 26561800
8. It is likely that a plastic change in GFAP expression in astrocytes selectively occurs around Oxytocin (OXT) neurons at proestrus and facilitates OXT release. PMID: 26994384
9. investigated temporal profile of astrocytic and neuronal injury markers after TBI; different mechanisms underlie clearance of UCH-L1 and GFAP in CSF and serum PMID: 25763798
10. Its antibody is able to protect cells from oxidative stress, which is due to changed protein expressions of the actin cytoskeleton. PMID: 25837926
11. GFAP release in hippocampus is significantly increased in a model of traumatic brain injury. PMID: 25898931
12. Hippocampal glucose uptake defects correlate with NeuN immunoreactivity in the latent phase and GFAP immunoreactivity in the chronic phase. PMID: 25798055
13. paracrine factors inhibit p38 MAPK and JNK, and most likely by regulating their downstream targets, p53 and STAT1, to promote astrocyte survival associated with GFAP downregulation after ischemic stroke in vitro PMID: 25752945
14. The down-regulation of histone deacetylase SIRT1 abrogating the effect of cocoa on glial fibrillary acidic protein up-regulation and on Lys310-RelA/p65 acetylation by silencing or blockage was clearly demonstrated herein in vivo and in vitro. PMID: 25448608
15. Data indicate that glial fibrillary acidic protein (GFAP) was up-regulated in satellite glial cells (SGCs) in dorsal root ganglia 14 days after streptozotocin injection. PMID: 25312986
16. increased in nucleus ambiguous after recurrent or superior laryngeal nerve injury PMID: 25181319
17. Diametric expression of GFAP and a different morphological pattern of caspase-3 labelling, although no changes in the cell number, were observed in the neurons of young and old animals. PMID: 25182537
18. Progesterone promotes neuroprotection following traumatic brain injury by inhibiting the expression of Nogo-A and GFAP, and increasing GAP-43 expression. PMID: 24567055
19. GFAP expression study also showed that cortical layer I usually contained multiple large astrocytes with branching processes, as well as numerous smaller processes with high intensity of expression. PMID: 25282817
20. GFAP repression is mediated through direct binding of p-PPARgamma (S112) to its promoter region. PMID: 24481447
21. GFAP is an important marker in determining the severity of traumatic brain injury. PMID: 24379073
22. To clarify whether GFAP-positive neoplastic astrocytes exist in rat spontaneous oligodendrogliomas and mixed gliomas or not, immunohistochemical examination was performed on spontaneous oligodendrogliomas (26 cases) and mixed gliomas. PMID: 23076037
23. Findings indicate the reciprocal relationships between GFAP expression and astrocyte neurotrophic activity by linking the ERalpha to ERbeta ratio to GFAP expression. PMID: 23515288
24. TMJ inflammation induced significant upregulation of GFAP (an astroglial marker) in the trigeminal subnucleus caudalis. PMID: 23110394
25. Western blot analysis showed that the chronic stress downregulated GFAP but upregulated NDRG2 protein PMID: 22610521
26. In the hippocampus, thalamus, and piriform cortex both vascular laminin and astrocytic GFAP expression are upregulated following kainic acid injection. PMID: 22475395
27. Increases in c-fos and GFAP were triggered by the combined stress of non-thermal irradiation and the toxic effect of picrotoxin on cerebral tissues. PMID: 21524663
28. There was a reduction in GFAP levels in the hippocampus of non-trained diabetic animals, which was not found in trained diabetic group. PMID: 21892662
29. Zuogui Pill may have protective effects on the optic nerve and retina ganglion cells after contusion by promoting nestin and GFAP expressions in Muller cells of the retina. PMID: 21906524
30. Activation of a pro-survival IL-6/JAK2/STAT3 cascade contributes to cholera toxin-induced GFAP expression. PMID: 21470923
31. Cesarean section might increase GFAP expression in the hippocampus and frontal cortex, and trigger neuronal apoptosis of hippocampus region. PMID: 21211284
32. GFAP-positive temporomandibular joint disc cells were characterized by broader processes and existed exclusively in the deeper area. PMID: 21679183
33. GFAP expression was down-regulated while GAP-43 expression upregulated in the retinal ganglial cells after peripheral nerve transplantation. PMID: 20423852
34. Region-specific alterations in the gene expression of GFAP, VEGF, and FGF-2 and their receptors in the aged brain correspond to changes in astrocytic reactivity, supporting astrocytic heterogeneity and demonstrating a differential aging effect. PMID: 21385309
35. GFAP content was diminished after a long-term administration of progesterone in hippocampus. PMID: 21683086
36. There is a differential expression pattern of GFAP in the rat brain during pregnancy and the beginning of lactation that is associated with changes in brain function during these reproductive stages. PMID: 20732387
37. Data show that astrocyte structural proteins GFAP and vimentin are induced by chronic leptin administration, suggesting astrocytes participating in leptin modulated synaptic inputs. PMID: 21343257
38. Butylphthalide may protect the neuron-vascular unit of the hippocampus of Alzheimer model rats by inhibiting the expression of GFAP and increasing the expression of VEGF. PMID: 20197608
39. Pax3 negatively regulates GFAP expression during astrocyte differentiation in vitro PMID: 21371476
40. these results indicate that intracellular cAMP levels regulate the expression of NPP1 in rat C6 glioma cells by a signalling pathway that is different from the GFAP signal transduction pathway PMID: 21168404
41. hyperbaric oxygenation treatment for cerebral infarction, can increase the expression of Map-2 and decrease the expression of GFAP. PMID: 19141977
42. Amniotic fluid-GFAP levels appear to correlate with spinal cord injury as gestation proceeds in fetal rats with myelomeningocele. PMID: 21284970
43. Retinal injury induces an upregulation of a complement of four intermediate filament proteins, including synemin and nestin, in Muller cells. PMID: 21139996
44. Chondroitinase ABC can reduce the expression of GFAP after spinal cord injury. PMID: 21046809
45. retinoic acid and cytokines have a synergistic effect on the regulation of glial fibrillary acidic protein expression PMID: 20876578
46. There was no significant increase in GFAP immunoreactivity in the white matter in a weight drop model of mild cerebral contusion injury in the rat. PMID: 20479526
47. Alcohol could repress the expression of GFAP and S100 of astrocytes. PMID: 16986516
48. S100 protein and glial fibrillary acidic protein expression increased significantly in the hippocampal astrocytes of rats with Alzheimer disease, and were inhibited by butylphthalide. PMID: 19726345
49. Brain concussion induced the expression of GFAP. PMID: 16850587
50. expression of 25-kDa synaptosomal-associated protein and glial fibrillary acidic protein, were significantly enhanced in the cerebellum of rats with treadmill training after cerebral infarct PMID: 19661770
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亞細胞定位：

Cytoplasm.
蛋白家族：

Intermediate filament family
組織特異性：

Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment.
數(shù)據(jù)庫鏈接：

KEGG: rno:24387

STRING: 10116.ENSRNOP00000032389

UniGene: Rn.91512