MCAM

黑色素瘤細(xì)胞黏附分子（MCAM），又稱CD146或MUC18，是一種跨膜糖蛋白，屬于免疫球蛋白超家族成員。它廣泛表達(dá)于內(nèi)皮細(xì)胞、黑色素瘤及多種實(shí)體瘤細(xì)胞表面，通過(guò)介導(dǎo)細(xì)胞間黏附、調(diào)控血管生成和腫瘤微環(huán)境重塑參與疾病進(jìn)程。MCAM與Wnt/β-catenin、PI3K/AKT等關(guān)鍵通路存在交互作用，能夠增強(qiáng)腫瘤細(xì)胞的遷移侵襲能力，其在循環(huán)腫瘤細(xì)胞表面的高表達(dá)被認(rèn)為是癌癥轉(zhuǎn)移的重要標(biāo)志。
臨床上，MCAM的異常表達(dá)與黑色素瘤、三陰性乳腺癌、肝癌等多種惡性腫瘤的進(jìn)展及不良預(yù)后顯著相關(guān)。近年來(lái)針對(duì)該靶點(diǎn)的藥物開發(fā)取得進(jìn)展，例如人源化單抗ABX-MA1已進(jìn)入針對(duì)轉(zhuǎn)移性黑色素瘤的Ⅰ期臨床試驗(yàn)，其通過(guò)阻斷MCAM介導(dǎo)的血管新生抑制腫瘤生長(zhǎng)。基于MCAM的CAR-T細(xì)胞療法在動(dòng)物模型中顯示出抗胰腺癌活性，但需進(jìn)一步驗(yàn)證其臨床安全性。該靶點(diǎn)同時(shí)涉及多發(fā)性硬化癥等自身免疫疾病，相關(guān)抑制劑研究為跨領(lǐng)域治療提供了新思路。

熱銷產(chǎn)品

Recombinant Human Cell surface glycoprotein MUC18 (MCAM), partial (CSB-RP104394h)

驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CD146 Recombinant Monoclonal Antibody (CSB-RA013563A0HU)

驗(yàn)證數(shù)據(jù)

Western Blot
Positive WB detected in A375 whole cell lysate
All lanes CD146 antibody at 0.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72 KDa
Observed band size: 120 KDa

IHC image of CSB-RA013563A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of U251 cells with CSB-RA013563A0HU at 1:37,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing A375 cells stained with CSB-RA013563A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Human soluble cluster of differentiation 146,sCD146 ELISA Kit (CSB-E11336h)

驗(yàn)證數(shù)據(jù)

貨號(hào)：CSB-E11336h

規(guī)格：96T/48T

靈敏度：0.31 ng/mL

檢測(cè)范圍：1.25 ng/mL-80 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

MCAM Antibody

MCAM Protein

MCAM ELISA Kit

MCAM Antibodies

MCAM for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA013563GA01HU	MCAM Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA10439C0Rb	MCAM Antibody, FITC conjugated	Human
CSB-PA10439A0Rb	MCAM Antibody	Human	ELISA, IHC
CSB-PA10439D0Rb	MCAM Antibody, Biotin conjugated	Human	ELISA
CSB-PA10439B0Rb	MCAM Antibody, HRP conjugated	Human	ELISA
CSB-PA066991	MCAM Antibody	Human	ELISA,WB,IHC
CSB-PA783399	MCAM Antibody	Human	ELISA,WB,IHC
CSB-RA013563A0HU	CD146 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, IF, FC
CSB-MA013563A0m	CD146 Monoclonal Antibody	Human	ELISA, WB, IF, FC

MCAM Proteins

MCAM Proteins for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來(lái)源
CSB-YP013563HU CSB-EP013563HU CSB-BP013563HU CSB-MP013563HU CSB-EP013563HU-B	Recombinant Human Cell surface glycoprotein MUC18 (MCAM), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-RP104394h	Recombinant Human Cell surface glycoprotein MUC18 (MCAM), partial	E.coli
CSB-CF013563HU	Recombinant Human Cell surface glycoprotein MUC18 (MCAM)	in vitro E.coli expression system

MCAM Proteins for Mus musculus (Mouse)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來(lái)源
CSB-YP823168MO CSB-EP823168MO CSB-BP823168MO CSB-MP823168MO CSB-EP823168MO-B	Recombinant Mouse Cell surface glycoprotein MUC18 (Mcam), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

MCAM Proteins for Rattus norvegicus (Rat)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來(lái)源
CSB-YP884118RA CSB-EP884118RA CSB-BP884118RA CSB-MP884118RA CSB-EP884118RA-B	Recombinant Rat Cell surface glycoprotein MUC18 (Mcam), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli