SIRPA
SIRPA，即信號調節蛋白α，別名Sirp-alpha-3p84、CD172a。它是一種I型跨膜蛋白，屬于免疫球蛋白超家族成員，主要在髓系細胞如巨噬細胞、單核細胞、樹突狀細胞等表面表達，也在神經細胞表面表達。SIRPA的作用機制是通過與CD47等配體結合，傳遞抑制性信號，抑制巨噬細胞的吞噬作用，從而在免疫調節中發揮重要作用。SIRPA的胞內結構域含有免疫受體酪氨酸抑制基序(ITIM)，能夠通過去磷酸化作用調節細胞內的信號通路。SIRPA參與了多種生理和病理過程的調節，包括免疫應答、細胞吞噬、中樞神經系統發育以及定向造血干細胞移植等。SIRPA通過與CD47的相互作用，可以抑制巨噬細胞對靶細胞的吞噬，這一機制被腫瘤細胞利用來實現免疫逃逸。因此，SIRPA是腫瘤免疫治療中的一個潛在靶點，尤其是與PD-1-PD-L1阻斷劑聯合使用時，可能對某些類型的癌癥治療具有重要意義。
熱銷產品
驗證數據
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized SIRPA at 2 μg/ml can bind human CD47(CSB-MP004940HU), the EC50 of human SIRPA protein is 58.30-85.04 ng/ml.
Human SIRPA protein His/Myc tag (CSB-MP021334HU) captured on COOH chip can bind Human CD47 protein Fc tag (CSB-MP004940HU) with an affinity constant of 19.1 nM as detected by LSPR Assay.
SIRPA Recombinant Monoclonal Antibody (CSB-RA443117A0HU)
驗證數據
IHC image of CSB-RA443117A0HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.13% DAB.
IHC image of CSB-RA443117A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.13% DAB.
Overlay Peak curve showing U937 cells surface stained with CSB-RA443117A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Human Tyrosine-protein phosphatase non-receptor type substrate 1(SIRPA) ELISA kit (CSB-EL021334HU)
驗證數據
貨號：CSB-EL021334HU
規格：96T/48T
靈敏度：0.039 ng/mL
檢測范圍：0.156 ng/mL-10 ng/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
SIRPA Antibodies
SIRPA for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA021334GA01HU | SIRPA Antibody | Human | ELISA,WB |
| CSB-PA021334LB01HU | SIRPA Antibody, HRP conjugated | Human | ELISA |
| CSB-PA021334LC01HU | SIRPA Antibody, FITC conjugated | Human | |
| CSB-PA021334LD01HU | SIRPA Antibody, Biotin conjugated | Human | ELISA |
| CSB-PA021334LA01HU | SIRPA Antibody | Human | ELISA, IHC, IF |
| CSB-PA004094 | SIRPA Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-RA443117A0HU | SIRPA Recombinant Monoclonal Antibody | Human | ELISA, IHC, FC |
SIRPA Proteins
SIRPA Proteins for Homo sapiens (Human)
SIRPA Proteins for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP021334MO1 CSB-EP021334MO1 CSB-BP021334MO1 CSB-MP021334MO1 CSB-EP021334MO1-B |
Recombinant Mouse Tyrosine-protein phosphatase non-receptor type substrate 1 (Sirpa), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF021334MO | Recombinant Mouse Tyrosine-protein phosphatase non-receptor type substrate 1 (Sirpa) | in vitro E.coli expression system |
SIRPA Proteins for Rattus norvegicus (Rat)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP021334RA1 CSB-EP021334RA1 CSB-BP021334RA1 CSB-MP021334RA1 CSB-EP021334RA1-B |
Recombinant Rat Tyrosine-protein phosphatase non-receptor type substrate 1 (Sirpa), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF021334RA | Recombinant Rat Tyrosine-protein phosphatase non-receptor type substrate 1 (Sirpa) | in vitro E.coli expression system |
SIRPA Proteins for Bos taurus (Bovine)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP021334BO1 CSB-EP021334BO1 CSB-BP021334BO1 CSB-MP021334BO1 CSB-EP021334BO1-B |
Recombinant Bovine Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF021334BO | Recombinant Bovine Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) | in vitro E.coli expression system |
SIRPA ELISA Kit
SIRPA ELISA Kit for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL021334HU | Human Tyrosine-protein phosphatase non-receptor type substrate 1(SIRPA) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 0.039 ng/mL |
