Human Malate Dehydrogenase 1 (MDH1) ELISA Kit
-
中文名稱：人蘋果酸脫氫酶1(MDH1)酶聯(lián)免疫試劑盒
-
貨號：CSB-E13662h
-
規(guī)格：96T/48T
-
價格：￥3600/￥2500
-
其他：
產(chǎn)品詳情
-
產(chǎn)品描述：人蘋果酸脫氫酶1(MDH1)酶聯(lián)免疫試劑盒（CSB-E13662h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的MDH1含量。MDH1即蘋果酸脫氫酶 1，是糖代謝關(guān)鍵酶。其催化蘋果酸與草酰乙酸相互轉(zhuǎn)化，參與三羧酸循環(huán)和蘋果酸-天冬氨酸穿梭。研究發(fā)現(xiàn)它與腫瘤代謝重編程密切相關(guān)，影響腫瘤細(xì)胞的增殖、存活，有望成為腫瘤治療新靶點。試劑盒檢測范圍為9.38 mU/mL-600 mU/mL，適用于細(xì)胞代謝機制研究、疾病模型能量通路分析、藥物干預(yù)效果評估等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
-
別名：cytoplasmic ELISA Kit; Cytosolic malate dehydrogenase ELISA Kit; Diiodophenylpyruvate reductase ELISA Kit; Malate dehydrogenase 1, NAD (soluble) ELISA Kit; Malate dehydrogenase ELISA Kit; Malate dehydrogenase cytoplasmic ELISA Kit; MDH s ELISA Kit; mdh1 ELISA Kit; MDHA ELISA Kit; MDHC_HUMAN ELISA Kit; MDHs ELISA Kit; MGC:1375 ELISA Kit; MOR2 ELISA Kit; Soluble malate dehydrogenase ELISA Kit
-
縮寫：MDH1
-
Uniprot No.：
-
種屬：Homo sapiens (Human)
-
樣本類型：serum, plasma, tissue homogenates, cell lysates
-
檢測范圍：9.38 mU/mL-600 mU/mL
-
靈敏度：2.35 mU/mL
-
反應(yīng)時間：1-5h
-
樣本體積：50-100ul
-
檢測波長：450 nm
-
研究領(lǐng)域：Metabolism
-
測定原理：quantitative
-
測定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human MDH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 91-99 1:2 Average % 102 Range % 97-107 1:4 Average % 110 Range % 107-113 1:8 Average % 86 Range % 83-89 -
回收率：
The recovery of human MDH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 88-94 EDTA plasma (n=4) 94 91-99 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mU/ml OD1 OD2 Average Corrected 600 2.157 2.198 2.178 2.053 300 1.766 1.738 1.752 1.627 150 1.255 1.269 1.262 1.137 75 0.764 0.798 0.781 0.656 37.5 0.512 0.531 0.522 0.397 18.75 0.305 0.324 0.315 0.190 9.38 0.198 0.188 0.193 0.068 0 0.126 0.124 0.125 -
數(shù)據(jù)處理：
-
貨期：3-5 working days
引用文獻(xiàn)
- Characterization of differentially expressed plasma proteins in patients with acute myocardial infarction Y Pan,Journal of Proteomics,2020
- Evaluation of Human Cerebrospinal Fluid Malate Dehydrogenase 1 as a Marker in Genetic Prion Disease Patients Zerr I, et al,Biomolecules,2019
- Regulation of human cerebrospinal fluid malate dehydrogenase 1 in sporadic Creutzfeldt-Jakob disease patients Schmitz M.et al,Aging (Albany NY). ,2016
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
-
功能：Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.
-
基因功能參考文獻(xiàn)：
- expression of miR-126-5p suppresses the enzymatic activity of MDH1, mitochondrial respiration and caused cell death in non-small cell lung carcinoma cell lines. PMID: 29574159
- Data show that in the endogenous readthrough of the human MDH1 stop codon, the stop codon can encode tryptophan and arginine, and is tissue-specific. PMID: 27881739
- Proliferating cells rely on both MDH1 and LDH to replenish cytosolic NAD. PMID: 28263970
- Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer. PMID: 27840030
- adipogenic differentiation may be regulated by the acetylation of MDH1 PMID: 22693256
- The MDH1 gene is not the cause of RP28-linked autosomal recessive retinitis pigmentosa. PMID: 20011630
- expression of MDH1 is maintained in the adult heart but is not present in levels as high as in the fetus PMID: 15565635
- Malate Dehydrogenase directly regulates the Tumor Suppressor Protein p53-dependent apoptosis upon glucose deprivation and involved in maintaining cellular metabolic state and further determining cell death. PMID: 19229245
顯示更多
收起更多
-
亞細(xì)胞定位：Cytoplasm.
-
蛋白家族：LDH/MDH superfamily, MDH type 2 family
-
數(shù)據(jù)庫鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
