MDH1 Antibody

中文名稱：

MDH1兔多克隆抗體
貨號：

CSB-PA013621EA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: HL60 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, LO2 whole cell lysate, NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue
All lanes: MDH1 antibody at 2.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 28, 39 kDa
Observed band size: 37 kDa

IHC image of CSB-PA013621EA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of U251 cells with CSB-PA013621EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) MDH1 Polyclonal antibody
Uniprot No.：

P40925
基因名：

MDH1
別名：

cytoplasmic antibody; Cytosolic malate dehydrogenase antibody; Diiodophenylpyruvate reductase antibody; Malate dehydrogenase 1, NAD (soluble) antibody; Malate dehydrogenase antibody; Malate dehydrogenase cytoplasmic antibody; MDH s antibody; mdh1 antibody; MDHA antibody; MDHC_HUMAN antibody; MDHs antibody; MGC:1375 antibody; MOR2 antibody; Soluble malate dehydrogenase antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human Malate dehydrogenase, cytoplasmic protein (1-334AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，MDH1 Antibody (CSB-PA013621EA01HU)，的標記方式是Non-conjugated。對于MDH1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA013621EB01HU	MDH1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA013621EC01HU	MDH1 Antibody, FITC conjugated
Biotin	CSB-PA013621ED01HU	MDH1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.
基因功能參考文獻：
1. expression of miR-126-5p suppresses the enzymatic activity of MDH1, mitochondrial respiration and caused cell death in non-small cell lung carcinoma cell lines. PMID: 29574159
2. Data show that in the endogenous readthrough of the human MDH1 stop codon, the stop codon can encode tryptophan and arginine, and is tissue-specific. PMID: 27881739
3. Proliferating cells rely on both MDH1 and LDH to replenish cytosolic NAD. PMID: 28263970
4. Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer. PMID: 27840030
5. adipogenic differentiation may be regulated by the acetylation of MDH1 PMID: 22693256
6. The MDH1 gene is not the cause of RP28-linked autosomal recessive retinitis pigmentosa. PMID: 20011630
7. expression of MDH1 is maintained in the adult heart but is not present in levels as high as in the fetus PMID: 15565635
8. Malate Dehydrogenase directly regulates the Tumor Suppressor Protein p53-dependent apoptosis upon glucose deprivation and involved in maintaining cellular metabolic state and further determining cell death. PMID: 19229245
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亞細胞定位：

Cytoplasm.
蛋白家族：

LDH/MDH superfamily, MDH type 2 family
數據庫鏈接：

HGNC: 6970

OMIM: 154200

KEGG: hsa:4190

STRING: 9606.ENSP00000438144

UniGene: Hs.526521

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