Recombinant Human Complement factor D(CFD) (Active)

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貨號：

CSB-MP005271HU
說明書：

MSDS Datasheet
規格：

￥1320
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CFD at 2 μg/ml can bind Anti-CFD recombinant antibody (CSB-RA005271MA1HU). The EC₅₀ is 31.86-39.21 ng/mL. Biological Activity Assay
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater?than?95%?as?determined?by?SDS-PAGE.
內毒素：

Less?than?1.0?EU/ug?as?determined?by?LAL?method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Human CFD at 2 μg/mL can bind Anti-CFD recombinant antibody (CSB-RA005271MA1HU). The EC50 is 31.86-39.21 ng/mL.
基因名：

CFD
Uniprot No.：

P00746
別名：

Complement factor D; EC:3.4.21.46; Adipsin; C3 convertase activator; Properdin factor D; CFD;DF, PFD
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

Mammalian cell
分子量：

25.9 kDa
表達區域：

26-253aa
氨基酸序列

ILGGREAEAHARPYMASVQLNGAHLCGGVLVAEQWVLSAAHCLEDAADGKVQVLLGAHSLSQPEPSKRLYDVLRAVPHPDSQPDTIDHDLLLLQLSEKATLGPAVRPLPWQRVDRDVAPGTLCDVAGWGIVNHAGRRPDSLQHVLLPVLDRATCNRRTHHDGAITERLMCAESNRRDSCKGDSGGPLVCGGVLEGVVTSGSRVCGNRKKPGIYTRVASYAAWIDSVLA
蛋白標簽：

C-terminal 10xHis-tagged
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized?from?a?0.2?μm?filtered?PBS,?6%?Trehalose,?pH?7.4
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
基因功能參考文獻：
1. MASP-3 is the exclusive pro-factor D activator in resting blood: the lectin and the alternative complement pathways are fundamentally linked. PMID: 27535802
2. disturbance of reciprocal relationships between adipsin and leptin in obesity is associated with the development of insulin resistance PMID: 28864895
3. TZDs (pioglitazone, rosiglitazone, and ciglitazone) slow tumor cell growth in vitro and in vivo with decreases in cell proliferation and increases in PPARg and adipsin PMID: 28219679
4. The strong association of PLIN1, CFD and ADIPOQ genes with adipogenesis prompted authors to study the influence the bone health status as evaluated by quantitative ultrasound (QUS) bone densitometer in a North Indian cohort. Overall, ADIPOQ (rs1501299 and rs3774261) and combined cluster of PLIN1 rs2304796 and rs2304795) and CFD (rs1683563) demonstrated correlation. PMID: 28189761
5. our findings set up a novel mechanism for FABP4, adipsin and adiponectin through gut microbiota mediating expression in gut Paneth cells. PMID: 26687459
6. Overall adipsin levels among male asbestos industry workers were significantly higher than among control subjects. PMID: 25840635
7. Data indicate that a urinary protein, adipsin, was significantly increased in patients with preeclampsia. PMID: 24958499
8. Study demonstrates that T2DM patients with beta cell failure are deficient in adipsin. PMID: 24995977
9. Complement factor D (FD) is activated by its substrate through interactions outside the active site which lock the unbound native state into an ordered inactive conformation via the self-inhibitory loop in FD. PMID: 24598742
10. We demonstrate novel secretion of adipsin and ASP by placental Hofbauer cells. PMID: 23956345
11. Increased activation of the alternative complement pathway in vitreous was controlled by disease stage and genetic variation in the complement pathway, supporting a role for complement activation in macular degeneration disease pathogenesis. PMID: 22930722
12. Anti factor D Fab fragment inhibits FD proteolytic function by interfering with macromolecular substrate access rather than by inhibiting FD catalysis. PMID: 22362762
13. CFD regulates activation of the alternative complement pathway, which is implicated in age related macular degeneration pathogenesis. PMID: 22003108
14. CFD may not play a major role in the genetic susceptibility to age-related macular degeneration. PMID: 21139680
15. crystal structure of C3bB at 4 A and complex with factor D at 3.5 A; data show how factor B binding to C3b forms open "activation" state of C3bB; Factor D binds open conformation of factor B through a site distant from the catalytic center PMID: 21205667
16. As adiponectin and adipsin levels in CSF did not correlate with their levels in plasma, it seems that there could be a secondary intrathecal synthesis of these adipocytokines in multiple sclerosis PMID: 19538214
17. increased serum levels in patients with seasonal allergic rhinitis PMID: 19699327
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相關疾病：

Complement factor D deficiency (CFDD)
亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
數據庫鏈接：

HGNC: 2771

OMIM: 134350

KEGG: hsa:1675

STRING: 9606.ENSP00000332139

UniGene: Hs.155597