Recombinant Human E3 ubiquitin-protein ligase RNF31 (RNF31), partial

中文名稱：

人RNF31重組蛋白
貨號：

CSB-YP856927HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人RNF31重組蛋白
貨號：

CSB-EP856927HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人RNF31重組蛋白
貨號：

CSB-EP856927HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人RNF31重組蛋白
貨號：

CSB-BP856927HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人RNF31重組蛋白
貨號：

CSB-MP856927HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

RNF31
Uniprot No.：

Q96EP0
別名：

E3 ubiquitin protein ligase RNF31; FLJ10111; FLJ23501; HOIL 1L interacting protein; HOIL-1-interacting protein; HOIL1L interacting protein; HOIP; RING finger protein 31; RNF31; RNF31_HUMAN; ZIBRA; Zinc in between ring finger ubiquitin associated domain; Zinc in-between-RING-finger ubiquitin-associated domain protein
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. RNF31 is required for linear ubiquitination of BCL10, thereby promoting TCR-induced NF-kappa-B activation. Binds polyubiquitin of different linkage types.
基因功能參考文獻：
1. The binding of SHARPIN or HOIL-1L facilitates the E2 loading of HOIP. PMID: 28978479
2. SPATA2 has been described as a previously unrecognized factor in the linear ubiquitin chain assembly complex-dependent signaling pathways that serves as an adaptor between HOIP and CYLD, thereby enabling recruitment of CYLD to signaling complexes. PMID: 27545878
3. This review highlights recent discoveries on RNF31 functions in nuclear factor modifications, breast cancer progression and possible therapeutic inhibitors targeting RNF31. [review] PMID: 27460922
4. The data reveal SPATA2 as a high-affinity binding partner of CYLD and HOIP, and a regulatory component of linear ubiquitin chain assembly complex-mediated NF-kappaB signaling. PMID: 27591049
5. LUBAC components control TLR3-mediated innate immunity, thereby preventing development of immunodeficiency and autoinflammation. PMID: 27810922
6. Study found that RNF31 is cleaved under apoptosis conditions through various stimulations; findings elucidate a novel regulatory loop between cell death and the survival signal PMID: 27669734
7. These results indicate that caspase-mediated cleavage of HOIP divides critical functional regions of HOIP, and that this regulates linear (de)ubiquitination of substrates upon apoptosis. PMID: 28189684
8. RNF31 decreased p53 stability. RNF31 depletion caused cell cycle arrest and cisplatin-induced apoptosis in a p53-dependent manner. RNF31 associated with the p53/MDM2 complex, facilitating p53 polyubiquitination and degradation by stabilizing MDM2. PMID: 26148235
9. This study describes a novel posttranslational regulation of the HOIP-containing linear-ubiquitin-chain assembly complex (LUBAC)-mediated linear ubiquitination that is critical for specifically directing TLR4-mediated NF-kappaB activation. PMID: 26578682
10. These findings collectively indicated an oncogene role of RNF31 in PCa progression which can be regulated by miR-503, suggesting that RNF31 could serve as a potential prognostic biomarker and therapeutic target for PCa. PMID: 26231797
11. we identified BCL10 as a bona fide target of BCR-induced linear ubiquitylation and demonstrated an important role of the linear ubiquitin ligase HOIP in BCR-induced phosphorylation PMID: 26038114
12. crystal structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase mechanism and regulation PMID: 26789245
13. human HOIP is essential for the assembly and function of LUBAC, which includes HOIL-1, and for various processes governing inflammation and immunity in both hematopoietic and nonhematopoietic cells PMID: 26008899
14. Two rare germline polymorphisms affecting the LUBAC subunit RNF31 were identified and enriched among patients with activated B cell-like subtype of diffuse large B-cell lymphoma. PMID: 24491438
15. RNF31, via stabilizing ESR1 levels, controls the transcription of estrogen-dependent genes linked to breast cancer cell proliferation. PMID: 24441041
16. Phosphorylation of OTULIN prevents HOIP binding, whereas unphosphorylated OTULIN is part of the endogenous LUBAC complex. PMID: 24726323
17. HOIP binding to OTULIN is required for the recruitment of OTULIN to the TNF receptor complex. PMID: 24726327
18. target specificity toward NEMO is determined by multiple LUBAC components, whereas linear ubiquitin chain elongation is realized by a specific interplay between HOIP and ubiquitin. PMID: 24030825
19. crystal structure of the catalytic core of HOIP in its apo form and in complex with ubiquitin PMID: 24141947
20. The HOIP component of the linear ubiquitin assembly complex catalyzes the formation of M1-polyubiquitin chains in response to interleukin-1. PMID: 23986494
21. The data showed how HOIP combines a general RING-IBR-RING ubiquitin ligase mechanism with unique, 'Linear ubiquitin chain Determining Domain'-dependent specificity for producing linear ubiquitin chains. PMID: 22863777
22. analysis of differentially expressed genes indicates systemic role for SF-1 in adrenal cortex affecting differentiation, proliferation and steroidogenesis and establishes RNF31 as an important regulator of adrenal steroidogenesis PMID: 22427816
23. The study highlights the versatility and specificity of protein-protein interactions involving Ub-like domain (UBL) of HOIL-1L/the Ub-associated domain (UBA) of HOIP and their cognate proteins. PMID: 22430200
24. ZIBRA was identified and its genomic organization, regulation, and expression studied in breast cancer cells. PMID: 15093743
25. RNF31 functions to stabilize DAX-1; RNF31 appears to be required for DAX-1 to repress transcription PMID: 19237537
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亞細胞定位：

Cytoplasm.
組織特異性：

Expressed in both normal and transformed breast epithelial cell lines.
數據庫鏈接：

HGNC: 16031

OMIM: 612487

KEGG: hsa:55072

STRING: 9606.ENSP00000315112

UniGene: Hs.375217